Gilbert Vella

In vivo heating of the guinea-pig fetal brain by pulsed ultrasound and estimates of thermal index.

Temperature was measured in the brain in live near-term fetal guinea pigs (62-66 d gestational age), during in utero exposure to a fixed beam of pulsed ultrasound at intensity ISPTA 2.82 W/cm2. Mean temperature increases of 4.3 degrees C close to parietal bone and 1.1 degrees C in the mid-brain were recorded after 2-min exposures. These values were lower (12%) than those obtained for ultrasound-induced heating near the bone in dead fetuses insonated in utero. A significant cooling effect of vascular perfusion was observed only when guinea pig fetuses reached late gestation, near term, when the cerebral vessels were well developed. The estimated value for the thermal index (TIB), as used in AIUM/NEMA output display standard, underestimated the measured temperature increase at the bone-brain interface. The ratio of measured temperature to the TIB is 1.3. A modification of the cranial thermal index provided a more reasonable, conservative, estimate of the temperature increase at a biologically significant point of interest at the brain-bone interface.

Ultrasound-induced temperature increase in guinea-pig fetal brain in utero: third-trimester gestation.

Temperature increase was measured at various depths in the brain of living fetal guinea pigs during in utero exposure to unscanned pulsed ultrasound at ISPTA 2.8 W/cm2. Mean temperature increases of 4.9 degrees C close to parietal bone and 1.2 degrees C in the midbrain were recorded after 2-min exposures. When exposures were repeated on the same sites in each fetus after death, the corresponding mean temperature increases were 4.9 degrees C and 1.3 degrees C, respectively. Cerebral blood perfusion had little cooling effect on ultrasound-induced heating in the guinea pig fetus of 57-61 days gestational age.

Ultrasound-induced heating in a foetal skull bone phantom and its dependence on beam width and perfusion

The cooling effect of single and multiple perfusing channels has been measured in a model of human foetal skull bone heated by wide and narrow beams of simulated pulsed spectral Doppler ultrasound (US). A focussed transducer operating with a centre frequency of 3.5 MHz, that emitted pulses of 5.7 micros duration with a repetition frequency of 8 kHz, was used. This produced a beam of power 100 +/- 2 mW with -6 dB diameters of 3.1 mm and 7.8 mm at 9 cm and 6 cm, respectively, from the transducer face. Arterial perfusion was simulated by allowing distilled water to flow in a large single channel or a grid of fine channels near the heated bone target. This study has established that: 1. perfusion-induced cooling is significantly enhanced when the bone phantom is heated by a wide rather than a narrow beam; 2. irrespective of the US beam width, a grid of small channels is more effective in cooling a heated bone target than a single larger diameter channel with the same volume flow rate; 3. the measured temperature rise and rate of temperature rise support the prediction of inverse proportionality to the US beam width; and 4. the perfusion time constants determined in our phantom model are 2 to 30 times larger than that assumed for the thermal index (TIB) algorithm.

Ultrasound-induced temperature increase in the guinea-pig fetal brain in vitro

The temperature of the brain of fetal guinea pigs was measured in vitro during exposure to an unscanned beam of pulsed ultrasound at intensity ISPTA 2.8 W/cm2. A mean temperature increase of 5.1 degrees C recorded after 2 min of insonation confirms results of an earlier similar study. The water-bath exposure system provided enhanced cooling of superficial tissue by acoustic streaming. When the scalp was removed, the ultrasound-induced temperature increase was substantially reduced (by 35%) due to cooling through radiation force-induced bulk fluid streaming along the direction of propagation in the water bath. The measured temperature increase in guinea pig fetal brain correlated with a modified cranial thermal index.

Effects of pulsed ultrasound on sphenoid bone temperature and the heart rate in guinea-pig foetuses

Temperature increase induced by exposure to unscanned pulsed ultrasound at an intensity (I(SPTA)) 2.82 W/cm2 was measured in the brain adjacent to the sphenoid bone of foetal guinea-pigs in late gestation under in vitro and in vivo (in utero) conditions. After 120 s exposure a mean temperature increase of 2.6 degrees C was measured in vitro. Removal of the overlying parietal bones increased this value to 5.2 degrees C. Mean temperature increases at the sphenoid bone recorded in utero were 1.5 degrees C live and 2.0 degrees C post mortem. Measurement of foetal ECG showed that ultrasound-induced heating of the hypothalamic region did not significantly alter foetal heart rate.

The cooling effect of liquid flow on the focussed ultrasound-induced heating in a simulated foetal brain

There is a need to investigate the thermal effects of diagnostic ultrasound (US) to assist the development of appropriate safety guidelines for obstetric use. The cooling effect of a single liquid flow channel was measured in a model of human foetal brain and skull bone heated by a focussed beam of simulated pulsed spectral Doppler US. Insonation conditions were 5.7 micros pulses, repeated at 8 kHz from a focussed transducer operating with a centre frequency of 3.5 MHz, producing a beam of -6 dB diameter of 3.1 mm at the focus and power outputs of up to 255 +/- 5 mW. Brain perfusion was simulated by allowing distilled water to flow at various rates in a 2 mm diameter wall-less channel in the brain soft tissue phantom material. This study established that the cooling effect of the flowing water; 1. was independent of the acoustic source power, 2. was more effective close to the flow channel, for example, there was a marked cooling at a distance of 1 mm and negligible cooling at a distance of 3 mm from the channel; and 3. initially increased at low flow rates, but further increase above normal perfusion had very little effect.

Do Students with Well-Aligned Perceptions of Question Difficulty Perform Better?

Effective criterion referenced assessment requires grade descriptors to clarify to students what skills are required to gain higher grades. But do students and staff actually have the same perception of the grading system, and if so, do they perform better than those whose perceptions are less accurately aligned with those of staff? Since students’ learning is influenced by their perception of the skills required to obtain high marks, it is important to learn what students think is being tested. The aims were thus to: 1. compare the student and staff perceptions of the grade descriptors associated with multiple‐choice questions (MCQs) and the factors that may underlie these student perceptions; 2. ascertain if students perform better when their perceptions of the grade descriptors are well‐aligned with staff judgements. Students studying biochemistry or physics were provided with appropriate online MCQs and were asked to indicate the correct content answers and their perception of the level of difficulty (as indicated by the appropriate grade descriptor) for each question. Detailed feedback on understanding of content and level of difficulty for each question was later provided online. A key finding is that student and staff agreement on perception of question difficulty is only about 50%, for either topic. The differences were significantly correlated with prior knowledge, entry level and exam mark for physics. The implications of these findings to the wider tertiary education community are discussed.