Gilles Ruffié

Rat fertility and embryo fetal development: Influence of exposure to the Wi-Fi signal

In recent decades, concern has been growing about decreasing fecundity and fertility in the human population. Exposure to non-ionizing electromagnetic fields (EMF), especially radiofrequency (RF) fields used in wireless communications has been suggested as a potential risk factor. For the first time, we evaluated the effects of exposure to the 2450MHz Wi-Fi signal (1h/day, 6days/week) on the reproductive system of male and female Wistar rats, pre-exposed to Wi-Fi during sexual maturation. Exposure lasted 3 weeks (males) or 2 weeks (females), then animals were mated and couples exposed for 3 more weeks. On the day before delivery, the fetuses were observed for lethality, abnormalities, and clinical signs. In our experiment, no deleterious effects of Wi-Fi exposure on rat male and female reproductive organs and fertility were observed for 1h per days. No macroscopic abnormalities in fetuses were noted, even at the critical level of 4W/kg.

Effects of Head-Only Exposure of Rats to GSM-900 on Blood-Brain Barrier Permeability and Neuronal Degeneration

Abstract Salford et al. reported in 2003 that a single 2-h exposure to GSM-900 mobile telephony signals induced brain damage (increased permeability of the blood-brain barrier and presence of dark neurons) 50 days after exposure. In our study, 16 Fischer 344 rats (14 weeks old) were exposed head-only to the GSM-900 signal for 2 h at various brain-averaged SARs (0, 0.14 and 2.0 W/kg) or were used as cage or positive controls. Albumin leakage and neuron degeneration were evaluated 14 and 50 days after exposure. No apoptotic neurons were found 14 days after the last exposure using the TUNEL method. No statistically significant albumin leakage was observed. Neuronal degeneration, assessed using cresyl violet or the more specific marker Fluoro-Jade B, was not significantly different among the tested groups. No apoptotic neurons were detected. The findings of our study did not confirm the previous results of Salford et al.

Effect of In Utero Wi-Fi Exposure on the Pre- and Postnatal Development of Rats

BACKGROUND The increase in exposure to the Wireless Fidelity (Wi-Fi) wireless communication signal has raised public health concerns especially for young people. Animal studies looking at the effects of early life and prenatal exposure to this source of electromagnetic fields, in the radiofrequency (RF) range, on development and behavior have been considered as high priority research needs by the World Health Organization. METHODS For the first time, our study assessed the effects of in utero exposure to a 2450 MHz Wi-Fi signal (2 hr/day, 6 days/week for 18 days) on pregnant rats and their pups. Three levels in terms of whole-body specific absorption rate were used: 0.08, 0.4, and 4 W/kg. The prenatal study on fetuses delivered by caesarean (P20) concerned five females/group. The dams and their offspring were observed for 28 days after delivery (15 females/group). RESULTS For all test conditions, no abnormalities were noted in the pregnant rats and no significant signs of toxicity were observed in the pre- and postnatal development of the pups, even at the highest level of 4 W/kg. CONCLUSIONS In the present study, no teratogenic effect of repeated exposures to the Wi-Fi wireless communication signal was demonstrated even at the highest level of 4 W/kg. The results from this screening study aimed at investigating Wi-Fi effects, strengthen the previous conclusions that teratology and development studies have not detected any noxious effects of exposures to mobile telephony-related RF fields at exposure levels below standard limits.

Human skin cell stress response to GSM-900 mobile phone signals. In vitro study on isolated primary cells and reconstructed epidermis

In recent years, possible health hazards due to radiofrequency radiation (RFR) emitted by mobile phones have been investigated. Because several publications have suggested that RFR is stressful, we explored the potential biological effects of Global System for Mobile phone communication at 900 MHz (GSM‐900) exposure on cultures of isolated human skin cells and human reconstructed epidermis (hRE) using human keratinocytes. As cell stress markers, we studied Hsc70, Hsp27 and Hsp70 heat shock protein (HSP) expression and epidermis thickness, as well as cell proliferation and apoptosis. Cells were exposed to GSM‐900 under optimal culture conditions, for 48 h, using a specific absorption rate (SAR) of 2 W·kg−1. This SAR level represents the recommended limit for local exposure to a mobile phone. The various biological parameters were analysed immediately after exposure. Apoptosis was not induced in isolated cells and there was no alteration in hRE thickness or proliferation. No change in HSP expression was observed in isolated keratinocytes. By contrast, a slight but significant increase in Hsp70 expression was observed in hREs after 3 and 5 weeks of culture. Moreover, fibroblasts showed a significant decrease in Hsc70, depending on the culture conditions. These results suggest that adaptive cell behaviour in response to RFR exposure, depending on the cell type and culture conditions, is unlikely to have deleterious effects at the skin level.

A phase signature for detecting wet subsurface structures using polarimetric L-band SAR

In this paper, we investigate the ability of L-band synthetic aperture radar (SAR) systems to penetrate soils to retrieve information about subsurface wet structures. Our experiment site, the Pyla dune, is a bare sandy area allowing high radar penetration and known to have large wet subsurface structures (paleosoils) at varying depths. Buried paleosoils, which act as moisture tanks, are detectable with radar, since they present a high permittivity due to their water content. By analyzing airborne polarimetric SAR data, we established that a phase signature is correlated to the buried wet palesoils: a phase difference of 23/spl deg/ between the horizontal (HH) and vertical (VV) channels was clearly observed. It allows detection of the paleosoil down to a larger depth (5.2 m) than when only considering HH and HV amplitude signals (3.5 m). In order to confirm this result, field measurements were performed that led to the same observed phase difference. We could fit our observations to the semiempirical model proposed by Oh and Sarabandi, and we reproduced the observed phenomenon using a two-layer integral equation method (IEM) model of the Pyla dune, which was completed by finite-difference time-domain (FDTD) numerical simulations. We show that the soil moisture significantly influences the radar response in terms of phase difference between the copolarized modes. Our study also shows that the single-scattering IEM model reproduces the observed phase difference fairly well for a natural outdoor site when combined to FDTD simulation results. This phase signature could be used as a new tool to map subsurface moisture in arid regions.

Local geoelectrical models of the Martian subsurface for shallow groundwater detection using sounding radars

Received 15 February 2002; revised 20 July 2002; accepted 28 August 2002; published 6 March 2003. [1] Low-frequency sounding radars should be able to probe the Martian subsurface layers down to varying depths, depending on the geoelectrical properties of the sounded sites. We present in this work four frequency-dependent geoelectrical models of the Martian subsurface in the 1–20 MHz frequency band, based on laboratory electromagnetic characterization of Martian soil analogues. Those models correspond to local Martian sites that we considered to be of particular interest in the search for water using mainly the Ground-Penetrating Radar (GPR) instrument of the Netlander mission. Results and discussion are also valid for both sounding experiments MARSIS and SHARAD. The four models of the Martian subsurface are designed to represent terrains where recent fluviallike features suggest the presence of near-subsurface ground ice and probably liquid water. We performed measurements on volcanic and sedimentary materials that may be present on these sites under the appropriate geophysical conditions that may exist in those terrains. We then simulated the backscattered radar echo arising from each site in the 2 MHz frequency band, using the Finite Difference Time Domain (FDTD) algorithm, in order to evaluate the instrument performances to probe the subsurface stratigraphy of each site. Our results confirm that the near-subsurface rich iron oxide mineralogy controls the instrument performances in terms of penetration depth and signal-to-noise ratio in the 2 MHz frequency band. We finally discuss the geophysical and geoelectrical sounding conditions that could lead to an ambiguous detection of shallow subsurface water on Mars for the Netlander GPR. INDEX TERMS: 3210 Mathematical Geophysics: Modeling; 1794 History of Geophysics: Instruments and techniques; 5144 Physical Properties of Rocks: Wave attenuation; 5109 Physical Properties of Rocks: Magnetic and electrical properties; KEYWORDS: Mars, hydrology, GPR, sounding, simulation, FDTD

Effect of GSM-900 and -1800 signals on the skin of hairless rats. III: Expression of heat shock proteins.

Purpose: We previously reported the inability of Global System for Mobile communication (GSM) signals at 900 (GSM-900) and 1800 (GSM-1800) MegaHertz (MHz) to induce morphological and physiological changes in epidermis of Hairless rats. The present work aimed at investigating heat shock proteins (HSP) expression – as a cellular stress marker – in the skin of Hairless rats exposed to GSM-900 and -1800 signals. Materials and methods: We studied the expression of the Heat-shock cognate (Hsc) 70, and the inducible forms of the Heat-shock proteins (Hsp) 25 and 70. Rat skin was locally exposed using loop antenna and restrain rockets to test several Specific Absorption Rates (SAR) and exposure durations: (i) single exposure: 2 hours at 0 and 5 W/kg; (ii) repeated exposure: 2 hours per day, 5 days per week, for 12 weeks, at 0, 2.5, and 5 W/kg. HSP expression was detected on skin slices using immunolabeling in the epidermal area. Results: Our data indicated that neither single nor repeated exposures altered HSP expression in rat skin, irrespective of the GSM signal or SAR considered. Conclusions: Under our experimental conditions (local SAR <5 W/kg), there was no evidence that GSM signals alter HSP expression in rat skin.

Effect of GSM-900 and -1800 signals on the skin of hairless rats. II: 12-week chronic exposures

Purpose: The purpose of this work was to determine whether the cellular components of Hairless-rat skin are affected by a chronic local exposure to non-ionizing radiations of Global Mobile Phone System: GSM-900 or -1800 radiations at specific absorption rate (SAR) 2.5 and 5 W/kg. Materials and methods: A selected part of the right back of five-week old female hairless rats was exposed or sham exposed (n = 8) for 2 h per day, 5 days a week, for 12 weeks to GSM-900 or -1800 signals using a loop-antenna. At the end of the experiment, skin biopsies were taken. Results: Analyses of skin sections using hematoxylin eosin saffron (HES) coloration showed no significant difference in skin thickness among the groups. Immunohistochemical analysis of basal lamella cells in radiofrequency radiation (RFR)-exposed epidermis showed that the ratio of the antigen Ki-67 (cellular proliferation marker) positive cells to total lamella cells remained within the range of the normal proliferation ratio. No significant differences in the level of filaggrin, collagen, and elastin were observed among the different groups. Conclusions: The results of this 12-week chronic study do not demonstrate major histological variations in the skin of hairless rats exposed to RFR used in mobile telephony (GSM-900 or -1800).

Impact of aerosols present in Titan's atmosphere on the CASSINI radar experiment

Simulations of Titans atmospheric transmission and surface reflectivity have been developed in order to estimate how Titans atmosphere and surface properties could affect performances of the Cassini radar experiment. In this paper we present a selection of models for Titans haze, vertical rain distribution, and surface composition implemented in our simulations. We collected dielectric constant values for the Cassini radar wavelength (

Performances of ground penetrating radars in arid volcanic regions: Consequences for Mars subsurface exploration

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