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Dive into the research topics where Gisela Masachessi is active.

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Featured researches published by Gisela Masachessi.


Archives of Virology | 2007

Picobirnavirus (PBV) natural hosts in captivity and virus excretion pattern in infected animals

Gisela Masachessi; Laura C. Martinez; Miguel O. Giordano; Patricia A. Barril; B. M. Isa; Leonardo J. Ferreyra; D. Villareal; M. Carello; C. Asis; Silvia V. Nates

Summary.A picobirnavirus (PBV) analysis was carried out by polyacrylamide gel electrophoresis of 513 stool samples obtained from 150 animal species collected from the Córdoba city zoo. The purpose of the present study was to determine susceptible species for PBV infection, the viral excretion pattern in infected animals, and the potential association with PBV diarrheic illness.Our findings suggest that PBVs are widespread in animals and could have a similar excretion behavior to that previously detected in infected humans. No disease association with PBV infection could be demonstrated. Thus, infected animals could be persistently infected asymptomatic carriers and could serve as reservoirs of infection.


Reviews in Medical Virology | 2012

Picobirnavirus infections: Viral persistence and zoonotic potential

Balasubramanian Ganesh; Krisztián Bányai; Vito Martella; Ferenc Jakab; Gisela Masachessi; Nobumichi Kobayashi

Picobirnaviruses (PBVs) are small, non‐enveloped, bisegmented double‐stranded RNA genomic viruses of vertebrate hosts. Since their discovery in the late 1980s in clinical specimens from outbreaks of acute gastroenteritis in children, significant efforts have been made to investigate the role of PBV in diarrheic diseases. PBV has been detected in sporadic episodes of diarrhea as sole pathogen or coinfection as well as in outbreaks of acute gastroenteritis and in immunocompromised patients with diarrhea. However, PBV is frequently detected in non‐diarrheic healthy hosts, and prolonged shedding has been observed in some individuals. Of interest, similar patterns of PBV infection have also been observed in pigs and other animal hosts. The increasing amount of PBV sequence data gathered from molecular epidemiological studies has evidenced a great sequence diversity of PBVs in various hosts and environmental samples. Importantly, evidence has been found for genetic relatedness between human and animal PBV strains, suggesting extant crossing points in the ecology and evolution of heterologous PBV strains. At present, no cell culture and animal model exists for PBVs. Well‐structured epidemiological studies are still the only alternative to demonstrate the potential etiological role of PBVs in acute gastroenteritis or other diseases. This review aims to analyze the public health aspects of PBV infection, especially its possible association with zoonosis. Copyright


Journal of Medical Virology | 2010

Correlation between rotavirus A genotypes detected in hospitalized children and sewage samples in 2006, Córdoba, Argentina

Patricia A. Barril; Miguel O. Giordano; María B. Isa; Gisela Masachessi; Leonardo J. Ferreyra; A.A. Castello; Graciela Glikmann; Silvia V. Nates

Routine rotavirus A (RV‐A) surveillance is based on clinical cases, so only symptomatic infections are reported. The objective of this study was to determine whether the RV‐A genotypes and cold seasonal pattern described in patients with diarrhea is reflected by sewage surveillance, which could be representative of the RV‐A genotypes circulating in the population. The genotype distribution of RV‐A in effluent samples from a local sewage treatment plant was compared to those from local clinical cases. A total of 52 sewage samples and 70 stool specimens from children with acute non‐bacterial diarrhea were collected from January to December 2006. The effluent specimens were concentrated and RNA extracts from concentrated sewage and clinical samples were genotyped for the rotavirus VP7 gene. The proportional distribution of the RV‐A G‐genotypes in sewage and clinical samples during the cold season was similar: G1 accounted for 26.6% of the typed sewage isolates and 28.8% of the clinical infections; G3 type accounted for 21.9% and 25.8%; G2 type 15.6% and 10.6%; G4 type 17.2% and 21.2%; G8 type 1.6% and 0%; and the G9 type 17.2% and 13.6%, respectively. A similar picture of RV‐A genotype detection was obtained in sewage samples collected during the cold and warm seasons. The results indicate that there is a correlation between genotypes of RV‐A isolates from human diarrheic patients and of those from sewage samples. In addition, sewage monitoring highlighted the uniform all‐year RV‐A circulation, which was in contrast to the peak incidence of RV‐A infection in the community. J. Med. Virol. 82: 1277–1281, 2010.


Infection, Genetics and Evolution | 2010

Picobirnavirus causes persistent infection in pigs.

Laura C. Martinez; Gisela Masachessi; Gabriela Carruyo; Leonardo J. Ferreyra; Patricia A. Barril; María B. Isa; Miguel O. Giordano; Juan E. Ludert; Silvia V. Nates

A study aimed to further understand the biology of porcine picobirnaviruses (PBV) was conducted between November 2003 and January 2008, on a farm located in the outskirts of Córdoba City, Argentina. PBV prevalence was examined by polyacrylamide gel electrophoresis and silver staining (PAGE S/S) on a total of 265 samples collected from pigs divided into four groups, according to age and physiological status. PBV detection rate was highest in the group of sows sampled within the lactogenic period (38.02%; p<0.05), followed by pregnant sows (15.09%), piglets aged 2-5 months of age (18.42%) and adult (> or =50 weeks) male pigs (0%). In addition, 103 samples collected in 3 follow-up studies were analyzed by PAGE S/S and reverse transcription followed by PCR (RT-PCR). Two of these studies followed female pigs from weaning up to slaughter and a third one from weaning up to 4 pregnancy periods. The results provide evidence that PBV establishes a persistent infection in the host with periods of silence intermingled with periods of low and high viral excretion. High PBV excretion levels were detected by PAGE S/S and were conditioned by age (primary infection) and host physiological status. Low PBV excretion levels were detected by RT-PCR throughout the entire study period. Sequence analysis of selected amplicons indicated that the virus excreted through the follow-up study was the same. These results suggest that porcine PBV is maintained in nature by transmission from infected asymptomatic individuals to susceptible ones.


Environmental Research | 2015

Rotavirus seasonality in urban sewage from Argentina: effect of meteorological variables on the viral load and the genetic diversity.

Patricia A. Barril; T.M. Fumian; V.E. Prez; P.I. Gil; Laura C. Martinez; Miguel O. Giordano; Gisela Masachessi; María B. Isa; Leonardo J. Ferreyra; Viviana Ré; M. Miagostovich; Jorge V. Pavan; Silvia V. Nates

In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from Córdoba city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. RT-nested PCR was applied for rotavirus detection, and VP7/VP4 characterization and real-time PCR for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time PCR in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than 18°C and also higher, although not statistically different during drier weather. VP7 and VP4 gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.


Veterinary Research | 2011

Genogroup I picobirnavirus in diarrhoeic foals: Can the horse serve as a natural reservoir for human infection?

Balasubramanian Ganesh; Krisztián Bányai; Gisela Masachessi; Zornitsa Mladenova; Shigeo Nagashima; Souvik Ghosh; Seegekote Mariyappa Nataraju; Madhusudhan Pativada; Rahul Kumar; Nobumichi Kobayashi

Picobirnaviruses (PBV) are small, non-enveloped viruses with a bisegmented double-stranded RNA genome. In this study a PBV strain, PBV/Horse/India/BG-Eq-3/2010, was identified in the faeces of a 10 month old weaned female foal with diarrhoea in January 2010 from Kolkata, India. Surprisingly, sequence comparison and phylogenetic analysis of a short stretch of the RNA dependent RNA polymerase gene revealed close genetic relatedness (> 98% nucleotide identity) to a human genogroup I PBV strain (Hu/GPBV1) detected earlier from the same part of India. Our observations together with earlier findings on genetic relatedness between human and animal PBV warrant further studies on zoonotic potential.


Infection, Genetics and Evolution | 2011

Genetic and evolutionary characterization of norovirus from sewage and surface waters in Córdoba City, Argentina

María Dolores Fernández; Carolina Torres; Laura C. Martinez; Miguel O. Giordano; Gisela Masachessi; Patricia A. Barril; María B. Isa; Rodolfo Campos; Silvia V. Nates; Viviana Andrea Mbayed

Noroviruses (NoVs) are among the most common viral agents that cause gastroenteritis in humans of all ages worldwide. They are excreted in the feces and introduced into environmental waters as raw or treated sewage. In this work, sewage and water samples collected from the Suquía River in the city of Córdoba, Argentina, were evaluated for the presence of NoV. Phylogenetic analysis demonstrated that the main genotype detected was GII.4, belonging to the widely-distributed 2006b variant, followed by strains related to the putative recombinant GII.g virus. Detected NoVs were more phylogenetically related with recent viruses from other countries than with previous local sequences, suggesting a rapid and wide spread of viral strains that prevents a geographically structured phylogeny. A Bayesian coalescent analysis demonstrated that variants isolated in this work have a most recent common ancestor placed in 2007-2008 with estimated substitution rates of 3.7-5.8×10(-3)s/s/y. Environmental samples showed a mixture of both viral types, pointing up to the co-circulation and the risk of mixed infections and recombination. This is the first report on the detection and characterization of NoV in sewage and river water in Argentina.


Journal of Medical Virology | 2013

Genetic and antigenic evolution profiles of G1 rotaviruses in córdoba, Argentina, during a 27-year period (1980–2006)

Patricia A. Barril; Laura C. Martinez; Miguel O. Giordano; Gisela Masachessi; María B. Isa; Jorge V. Pavan; Graciela Glikmann; Silvia V. Nates

Rotavirus G1 strains represent the most common genotype that causes diarrhea in humans and has been incorporated into both, monovalent and multivalent, rotavirus licensed vaccines. The aim of this study was to determine the evolution profile of G1 rotaviruses in Córdoba, Argentina, over a 27‐year period (1980–2006). Intragenotype diversity, represented by lineages within rotavirus circulating strains, was observed. Phylogenetic analysis of the VP7‐gene of G1 rotavirus clinical strains showed the circulation of G1 lineage IV and V strains in the 1980s, and co‐circulation of lineage I and II strains in the 1990s and 2000–2006. The distribution of G1 in lineages could be linked to multiple nucleotide substitutions distributed across lineages that did not correlate with the emergence of G1 antigenic variants. Moreover, temporal lineage distribution was not linked to significant changes in G1 prevalence. Therefore, the continuous and dominant circulation of G1 over time could not be related to the emergence of antigenic variants in the community. Continuous rotavirus surveillance is necessary to understand rotavirus evolution and to measure how genetic and antigenic changes might affect the effectiveness of vaccines in the future. J. Med. Virol. 85:363–369, 2013.


Archives of Virology | 2010

A novel human adenovirus hexon protein of species D found in an AIDS patient

Leonardo J. Ferreyra; Miguel O. Giordano; Laura C. Martinez; M. Beatriz Isa; Patricia A. Barril; Gisela Masachessi; Sergio Grutadauria; Jorge V. Pavan; Silvia V. Nates

To date, human adenoviruses are classified into 53 types (types 1–51 and types 53 and 54), which have been grouped into six species named A through F, and the recently identified type 52 has been proposed as member of a new species, G. Type classification is based on type-specific epitopes within loop 1 (L1) and loop 2 (L2) of the hexon protein, which contain seven hypervariable regions that are responsible for type specificity. In this paper, we present the characterization of an adenovirus strain isolated from a male AIDS patient in Cordoba, Argentina. This strain was found to be a member of species D by genomic Sma I restriction analysis. Sequencing of the L1 and L2 regions of the hexon gene and immunological characterization by virus neutralization revealed this hexon to be unique and distinct from the previously identified hexons of types within species D. A seroepidemiologic study in the human population of Cordoba showed that this strain was not endemic in the local human population.


Science of The Total Environment | 2015

Quantification of human infection risk caused by rotavirus in surface waters from Córdoba, Argentina.

V.E. Prez; P.I. Gil; C.F. Temprana; P.R. Cuadrado; Laura C. Martinez; Miguel O. Giordano; Gisela Masachessi; María B. Isa; Viviana Ré; Jorge V. Pavan; Silvia V. Nates; Patricia A. Barril

Fecal contamination of water is a worrying problem because it is associated with the transmission of enteric pathogenic microorganisms that can cause many infectious diseases. In this study, an environmental survey was conducted to assess the level of viral contamination by viable enterovirus and rotavirus genome in two recreational rivers (Suquía and Xanaes) of Córdoba, Argentina. Quantitative microbial risk assessment (QMRA) was calculated to estimate the risk of rotavirus infection. Water sampling was carried out during a one-year period, the presence of total and fecal coliforms was determined and water samples were then concentrated for viral determination. Cell culture and indirect immunofluorescence were applied for enterovirus detection and RT-qPCR for rotavirus quantification. Coliform bacteria levels found in Suquía River often far exceeded the guideline limits for recreational waters. The Xanaes exhibited a lower level of bacterial contamination, frequently within the guideline limits. Enterovirus and rotavirus were frequently detected in the monitoring rivers (percentage of positive samples in Suquía: 78.6% enterovirus, 100% rotavirus; in Xanaes: 87.5% enterovirus, 18.7% rotavirus). Rotavirus was detected at a media concentration of 5.7×10(5) genome copies/L (gc/L) in the Suquía and 8.5×10(0)gc/L in the Xanaes. QMRA revealed high risk of rotavirus infection in the Suquía, at sampling points with acceptable and non-acceptable bacteria numbers. The Xanaes showed significantly lower health risk of rotavirus infection but it proved to be a public health hazard. The viral occurrence was not readily explained by the levels of bacteria indicators, thus viral monitoring should be included to determine microbiological water quality. These findings provide the first data of QMRA for recreational waters in Argentina and reveal the need for public awareness of the health implications of the use of the river waters.

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Silvia V. Nates

National University of Cordoba

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Miguel O. Giordano

National University of Cordoba

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Laura C. Martinez

National University of Cordoba

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Patricia A. Barril

National University of Cordoba

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María B. Isa

National University of Cordoba

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Leonardo J. Ferreyra

National University of Cordoba

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Jorge V. Pavan

National University of Cordoba

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Viviana Ré

National University of Cordoba

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V.E. Prez

National University of Cordoba

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Balasubramanian Ganesh

Indian Council of Medical Research

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