Giulia Maria Mariani

Soft tissue and crestal bone changes around implants with platform-switched abutments placed nonsubmerged at subcrestal position: a 2-year clinical and radiographic evaluation.

PURPOSE To evaluate crestal bone changes around implants with platform-switched abutments placed 1 mm subcrestally in a prospective clinical investigation. MATERIALS AND METHODS Forty consecutive systemically healthy patients (mean age ± standard deviation [SD], 55.2 ± 8.7 years) with one or more missing teeth were consecutively treated with 1-mm subcrestally positioned, platform-switched, tapered, full treated implants restored with coded abutments. A total of 58 implants were placed. Final restorations were delivered 4 to 8 months after implant insertion. Digital standardized periapical radiographs using customized film holders were obtained at the time of implant insertion, and at 12 and 24 months after final prosthesis placement. Marginal peri-implant bone levels were measured at the mesial and distal surfaces of each implant using digital image software. RESULTS All implants osseointegrated and were clinically stable at the 2-year follow-up. The cumulative survival rate was 100%. From implant insertion to the 2-year follow-up, the mean bone loss was 0.32 ± 0.37 mm. No significant differences related to sex, implant site, and bone density were observed. The mean midbuccal and interproximal soft tissue margin positions were 1.13 ± 0.5 mm and 1.15 ± 0.6 mm coronal to the prosthetic finish line, respectively. CONCLUSION There is limited clinical information regarding the amount of marginal bone loss around two-piece platform-switched implants placed at subcrestal positions. Results of this study suggest that platform switching and subcrestal location of the implant-abutment interface may be effective in reducing bone loss and in preserving esthetics around dental implants.

Osseous resective surgery with and without fibre retention technique in the treatment of shallow intrabony defects: a split‐mouth randomized clinical trial

AIM The aim of this split-mouth clinical trial was to compare the effectiveness of Apically Positioned Flap with Fibre Retention Osseous Resective Surgery (FibReORS) or Osseous Resective Surgery (ORS) in the treatment of periodontal pockets associated with intrabony defects ≤ 3 mm at posterior natural teeth. MATERIALS AND METHODS Twenty-six posterior sextants requiring osseous resective surgery were selected in 13 chronic periodontitis patients: 13 sextants were randomly assigned to ORS and 13 to FibReORS. Clinical evaluation of probing depth (PD), gingival recession and clinical attachment level was performed at baseline, 6 and 12 months postoperatively. Periapical radiographs were taken prior and after surgical treatment, at 6- and 12-month follow-up. RESULTS Ostectomy amounted to 1.0 ± 0.3 mm in the ORS group and to 0.4 ± 0.2 mm in the FibReORS group. At 12-month examination PD changes did not significantly differ between the experimental groups. ORS group showed significantly (p < 0.001) greater clinical attachment loss (2.2 ± 1.0 mm versus 1.0 ± 0.6 mm), radiographic bone resorption (0.43 ± 0.08 mm versus 0.13 ± 0.09 mm) and post-operative patient discomfort compared to FibReORS. CONCLUSION FibReORS resulted in similar PD reduction, but less ostectomy, clinical attachment loss and patient morbidity compared to ORS.

Increased early inflammatory response and osteoclastic activity in gingival tissues following conventional osseous resective surgery compared with the fibre retention technique: a pilot study

BACKGROUND AND OBJECTIVE The retention of suprabony connective fibres attached into the root cementum during fibre retention osseous resective surgery (FibReORS) results in a more conservative intrasurgical removal of bone, and limits further bone loss and patient morbidity during healing, compared with conventional osseous resective surgery (ORS). This may be a result of the protective effect of preserved connective tissue over the interproximal sites and the lower activation of the inflammatory mechanisms. Thus, the aim of this pilot study was to compare the expression of inflammatory and osteoclastic activity markers in gingival tissues following FibReORS and ORS in the early postsurgical phase. MATERIAL AND METHODS Twenty-six posterior sextants requiring osseous resective surgery were selected in 13 patients with chronic periodontitis: 13 sextants were randomly assigned to ORS and 13 to FibReORS in a split-mouth design. Gingival biospies were collected during the surgical sessions and at suture removal. Tissue samples were analysed to evaluate the expression of proinflammatory and immunity regulatory mediators (interleukin-1α, C-X-C motif chemokine ligand 5, interferon-γ and tumour necrosis factor-α), cluster of differentiation 14 (CD14; a monocyte/macrophage marker) and TRAP (an osteoclast marker) using immunohistochemical, immunofluorescence and cytofluorimetric analyses, respectively. RESULTS Postsurgery, a higher number of inflammatory cells and stronger expression of proinflammatory cytokines were observed in the epithelium and connective tissue of ORS gingival samples compared with FibReORS gingival samples (p < 0.001). This was accompanied by increased numbers of CD14-positive and TRAP-positive cells. CONCLUSION Retention of the supracrestal connective fibres appears to reduce the postsurgical intensity of the host-mediated inflammatory response.

Enamel Matrix Derivative Proteins in Combination with a Flapless Approach for Periodontal Regeneration of Intrabony Defects: A 2-Year Prospective Case Series.

The present case series evaluated the potential benefits of a flapless approach in the regenerative treatment of residual deep intrabony defects. In each of 11 patients, one periodontal defect with an intrabony component of ≥ 3 mm was treated regeneratively using enamel matrix derivative combined with a closed surgical technique. Clinical and radiographic parameters were recorded at baseline and at 12 and 24 months postoperatively. All defects experienced favorable clinical and radiographic outcomes at the 24-month follow-up. When considering only sites located in the anterior region, all experienced complete pocket closure.

Effect of non-surgical periodontal therapy on salivary metabolic fingerprint of generalized chronic periodontitis using nuclear magnetic resonance spectroscopy

OBJECTIVE Metabolomic analysis of saliva proved its accuracy in discriminating patients with generalized chronic periodontitis (GCP) from healthy subjects by identifying specific molecular signatures of the disease. There is lack of investigations concerning the effect of periodontal treatment on individual metabolic fingerprints. Therefore, the aim of this study was to determine whether non-surgical periodontal therapy could change salivary metabolomic profile in GCP to one more similar to periodontal health. DESIGN Unstimulated whole saliva of 32 controls and 19 GCP patients were obtained prior to and 3 months after conventional staged non-surgical periodontal therapy. Metabolic profiling was performed using Nuclear Magnetic Resonance (NMR) spectroscopy, followed by univariate and multivariate paired approaches to assess the changes introduced by the therapy. RESULTS In GCP group, periodontal treatment led to an improvement in all clinical parameters (p < 0.001). The accuracy of the multivariate model in discriminating the metabolomic profile of each GCP patient at two time points was 92.5%. Despite the almost perfect separation of the spectra in the metabolic space, the univariate analysis failed to identify significant variations in single metabolite content. The post-treatment metabolic profile of GCP patients could not be assimilated to that of healthy controls who exhibited different levels of lactate, pyruvate, valine, proline, tyrosine, and formate. CONCLUSIONS Based on these data, NMR-spectroscopic analysis revealed that, despite significant changes in the overall metabolomic fingerprint after non-surgical therapy, GCP patients maintained a distinctive metabolic profile compared to healthy individuals.