Giuliano Marchi

Organic acids in the rhizosphere and phytoavailability of sewage sludge-borne trace elements

The aim of this work was to quantify low molecular weight organic acids in the rhizosphere of plants grown in a sewage sludge-treated media, and to assess the correlation between the release of the acids and the concentrations of trace-elements in the shoots of the plants. The species utilized in the experiment were cultivated in sand and sewage sludge-treated sand. The acetic, citric, lactic, and oxalic acids, were identified and quantified by high performance liquid chromatography in samples collected from a hydroponics system. Averages obtained from each treatment, concentration of trace elements in shoots and concentration of organic acids in the rhizosphere, were compared by Tukey test, at 5% of probability. Linear correlation analysis was applied to verify an association between the concentrations of organic acids and of trace elements. The average composition of organic acids for all plants was: 43.2, 31.1, 20.4 and 5.3% for acetic, citric, lactic, and oxalic acids, respectively. All organic acids evaluated, except for the citric acid, showed a close statistical agreement with the concentrations of Cd, Cu, Ni, and Zn found in the shoots. There is a positive relationship between organic acids present in the rhizosphere and trace element phytoavailability.

Heavy metals extractability in a soil amended with sewage sludge

Few investigations have been carried out about the comparison of desorption rate and amount of heavy metals extracted successively by organic acid mixtures mimicking the rhizosphere and routine extractants in sewage sludge-amended soils. Extractions of Zn, Cd, Ni, Cr, and Cu were performed in samples of a sewage sludge-amended soil using seven extractants: four organic acid mixtures and three routine extractants (DTPA, Mehlich-I, and ammonium acetate). Results from single pass extractions, in which the extractable metal contents were determined by simply extracting the soil a single time, as well as from 15 successive extractions, in which the solid residues of the first extraction was successively extracted 14 additional times, of heavy metals were analyzed. The extractability of heavy metals in a single pass extraction was, in general, as follows: Mehlich-I > DTPA > organic acids > NH4OAc. The highest rates of extraction followed the general order: DTPA > Mehlich-I > organic acids > NH4OAc. While Mehlich-I presented the highest extractability of heavy metals among studied extractants, DTPA showed a high extractability of Zn, Cd, Ni, and Cu in a single extraction as well as the highest rates of extraction among the studied extractants. The transfer of heavy metals from soil to organic acid solutions is slower than to DTPA and Mehlich-I extractants.

Sulfate and chromate increased each other's uptake and translocation in As-hyperaccumulator Pteris vittata

We investigated the effects of chromate (CrVI) and sulfate on their uptake and translocation in As-hyperaccumulator Pteris vittata. Plants were exposed to 1) 0.1 mM CrVI and 0, 0.25, 1.25 or 2.5 mM sulfate or 2) 0.25 mM sulfate and 0, 0.5, 2.5 or 5.0 mM CrVI for 1 d in hydroponics. P. vittata accumulated 26 and 1261 mg kg(-1) Cr in the fronds and roots at CrVI0.1, and 2197 and 1589 mg kg(-1) S in the fronds and roots at S0.25. Increasing sulfate concentrations increased Cr root concentrations by 16-66% and helped CrVI reduction to CrIII whereas increasing CrVI concentrations increased frond sulfate concentrations by 3-27%. Increasing sulfate concentrations enhanced TBARS concentrations in the biomass, indicating oxidative stress caused lipid peroxidation in plant cell membranes. However, addition of 0.25-2.5 mM sulfate alleviated CrVIs toxic effects and decreased TBARS from 23.5 to 9.46-12.3 μmol g(-1) FW. Though CrVI was supplied, 78-96% of CrIII was in the biomass, indicating efficient CrVI reduction to CrIII by P. vittata. The data indicated the amazing ability of P. vittata in Cr uptake at 289 mg kg(-1) h(-1) with little translocation to the fronds. These results indicated that P. vittata had potential in Cr phytoremediation in contaminated sites but further studies are needed to evaluate this potential. The facts that CrVI and sulfate helped each other in uptake by P. vittata suggest that CrVI was not competing with sulfate uptake in P. vittata. However, the mechanisms of how sulfate and CrVI enhance each others accumulation in P. vittata need further investigation.

Efeito da adubação orgânica sobre as frações de carbono de solos cultivados com alfacen americana

Avaliou-se, neste estudo, o efeito da aplicacao de adubos orgânicos, minerais e calagem, nas fracoes humica, fulvica e nos teores de carbono orgânico de um Latossolo Vermelho e de um Cambissolo, cultivados com alface (americana). O experimento, realizado no Departamento de Ciencia do Solo da Universidade Federal de Lavras, foi constituido de cinco doses de material humico (0, 20, 40, 100 e 200 L ha-1), tres tipos de adubacao (composto orgânico, esterco de aves e mineral) e calagem (com e sem uso de calcario), em cinco repeticoes. No Cambissolo, a calagem contribuiu para a diminuicao do teor de C orgânico nas areas adubadas com fertilizante mineral e composto, e aumentou o armazenamento de C no solo adubado com esterco de aves. Em relacao a adubacao mineral, o uso de adubo orgânico aumentou os teores de C-fracao acido humico e a relacao C-humico/fulvico. No Latossolo, independente do tipo de adubacao, o uso da calagem propiciou o armazenamento no solo de mais C do que o observado na area sem correcao da acidez. Os efeitos da calagem e das fontes de nutrientes variaram em funcao da fracao orgânica analisada ou das relacoes entre seus teores de carbono.

Agronomic efficiency of potassium fertilization in lettuce fertilized with alternative nutrient sources

The aim of this study was to evaluate the effect of alternative sources of nutrients on the nutrition, yield and efficiency of potassium fertilization in lettuce. The experiment was carried out in a greenhouse, using 3.7 kg pots filled with a dystrophic red-yellow Latosol of medium texture. The experimental design was randomized, with treatments divided into a 4 x 6 factorial: four doses of potassium (0; 200; 400; 600 kg ha -1 K 2 O) and six alternative sources of nutrients (breccia, ultramafic, biotite schist, phlogopite, and mining and Chapada by-products), with four replications. Content and accumulation were determined for potassium (K), copper (Cu), zinc (Zn) and nickel (Ni) in the lettuce shoots, and from these data two indices were calculated for the efficiency of potassium as a fertilizer. The application of increasing values of alternative sources of nutrients promoted improvements in nutrition and increases in lettuce yield. The efficiency of potassium fertilization decreased with the increase in values of potassium taken from alternative nutrient sources, with the mining by-products and the ultramafic being superior to the other sources. Crushed silicate rocks and mining by-products can therefore both be used as fertilizer in organic and conventional production systems.

Emergence and initial growth of Urochloa Cultivars at different sowing depths

The emergence and growth of Urochloa spp. at different sowing depths was evaluated, considering the possibility of delaying the initial growth of forage grass by sowing it deeper to provide competitive advantage to the grain culture in crop-pasture association systems. The experiment was conducted in a greenhouse with U. brizantha cv. Marandu and cv. Piata and U. decumbens cv. Basilisk, and sowing depths of 0, 3, 6, 9, and 12 cm. Similar behavior between U. brizantha cultivars was observed for daily emerged seedlings, emergence percentage, height and dry matter of plants at 28 days after sowing (DAS); however, these results differed from those obtained for U. decumbens, except for the height of U. brizantha cv. Piata. The evaluated variables, for all cultivars, tended to be reduced when sown deeper than 6 cm. The delay in emergence at 0 cm without reduction, for all cultivars of Urochloa, did not result in reduced height and dry matter weight.The emergence and growth of Urochloa spp. at different sowing depths was evaluated, considering the possibility of delaying the initial growth of forage grass by sowing it deeper to provide competitive advantage to the grain culture in crop-pasture association systems. The experiment was conducted in a greenhouse with U. brizantha cv. Marandu and cv. Piata and U. decumbens cv. Basilisk, and sowing depths of 0, 3, 6, 9, and 12 cm. Similar behavior between U. brizantha cultivars was observed for daily emerged seedlings, emergence percentage, height and dry matter of plants at 28 days after sowing (DAS); however, these results differed from those obtained for U. decumbens, except for the height of U. brizantha cv. Piata. The evaluated variables, for all cultivars, tended to be reduced when sown deeper than 6 cm. The delay in emergence at 0 cm without reduction, for all cultivars of Urochloa, did not result in reduced height and dry matter weight.

Plant availability of trace elements in sewage sludge-treated soils: methodology¹

Synthetic root exudates were formulated based on the organic acid composition of root exudates derived from the rhizosphere of aseptically grown corn plants, pH of the rhizosphere, and the background chemical matrices of the soil solutions. The synthetic root exudates, which mimic the chemical conditions of the rhizosphere environment where soil-borne metals are dissolved and absorbed by plants, were used to extract metals from sewage-sludge treated soils 16 successive times. The concentrations of Zn, Cd, Ni, Cr, and Cu of the sludge-treated soil were 71.74, 0.21, 15.90, 58.12, and 37.44 mg kg-1, respectively. The composition of synthetic root exudates consisted of acetic, butyric, glutaric, lactic, maleic, propionic, pyruvic, succinic, tartaric, and valeric acids. The organic acid mixtures had concentrations of 0.05 and 0.1 mol L-1 -COOH. The trace elements removed by successive extractions may be considered representative for the availability of these metals to plants in these soils. The chemical speciation of the metals in the liquid phase was calculated; results showed that metals in sludge-treated soils were dissolved and formed soluble complexes with the different organic acid-based root exudates. The most reactive organic acid ligands were lactate, maleate, tartarate, and acetate. The inorganic ligands of chloride and sulfate played insignificant roles in metal dissolution. Except for Cd, free ions did not represent an important chemical species of the metals in the soil rhizosphere. As different metals formed soluble complexes with different ligands in the rhizosphere, no extractor, based on a single reagent would be able to recover all of the potentially plant-available metals from soils; the root exudate-derived organic acid mixtures tested in this study may be better suited to recover potentially plant-available metals from soils than the conventional extractors.