Giulio Gavini

Resistance to Flexural Fatigue of Reciproc R25 Files under Continuous Rotation and Reciprocating Movement

INTRODUCTION The aim of the present work was to evaluate the resistance to flexural fatigue of Reciproc R25 nickel-titanium files, 25 mm, used in continuous rotation motion or reciprocation motion, in dynamic assays device. METHODS Thirty-six Reciproc R25 files were divided into 2 groups (n = 18) according to kinematics applied, continuous rotary (group CR) and reciprocation motion (group RM). The files were submitted to dynamic assays device moved by an electric engine with 300 rpm of speed that permitted the reproduction of pecking motion. The files run on a rings groove of temperate steel, simulating instrumentation of a curved root canal with 40° and 5 mm of curvature radius. The fracture of file was detected by sensor of device, and the time was marked. The data were analyzed statistically by Students t test, with level of significance of 95%. RESULTS The instruments moved by reciprocating movement reached significantly higher numbers of cycles before fracture (mean, 1787.78 cycles) when compared with instruments moved by continuous rotary (mean, 816.39 cycles). CONCLUSIONS The results showed that the reciprocation motion improves flexural fatigue resistance in nickel-titanium instrument Reciproc R25 when compared with continuous rotation movement.

Evaluation of Radiopacity, pH, Release of Calcium Ions, and Flow of a Bioceramic Root Canal Sealer

INTRODUCTION The aim of the present study was to evaluate the physicochemical properties of a bioceramic root canal sealer, Endosequence BC Sealer. Radiopacity, pH, release of calcium ions (Ca(2+)), and flow were analyzed, and the results were compared with AH Plus cement. METHODS Radiopacity and flow were evaluated according to ISO 6876/2001 standards. For the radiopacity analysis, metallic rings with 10-mm diameter and 1-mm thickness were filled with cements. The radiopacity value was determined according to radiographic density (mm Al). The flow test was performed with 0.05 mL of cement placed on a glass plate. A 120-g weight was carefully placed over the cement. The largest and smallest diameters of the disks formed were measured by using a digital caliper. The release of Ca(2+) and pH were measured at periods of 3, 24, 72, 168, and 240 hours with spectrophotometer and pH meter, respectively. Data were analyzed by analysis of variance and Tukey test (P < .05). RESULTS The bioceramic endodontic cement showed radiopacity (3.84 mm Al) significantly lower than that of AH Plus (6.90 mm Al). The pH analysis showed that Endosequence BC Sealer showed pH and release of Ca(2+) greater than those of AH Plus (P < .05) during the experimental periods. The flow test revealed that BC Sealer and AH Plus presented flow of 26.96 mm and 21.17 mm, respectively (P < .05). CONCLUSIONS Endosequence BC Sealer showed radiopacity and flow according to ISO 6876/2001 recommendations. The other physicochemical properties analyzed demonstrated favorable values for a root canal sealer.

Effect of the Combination of Sodium Hypochlorite and Chlorhexidine on Dentinal Permeability and Scanning Electron Microscopy Precipitate Observation

INTRODUCTION This study compared the combined use of sodium hypochlorite (NaOCl) and chlorhexidine (CXH) with citric acid and CXH on dentinal permeability and precipitate formation. METHODS Thirty-four upper anterior teeth were prepared by rotary instrumentation and NaOCl. The root canal surfaces were conditioned for smear layer removal using 15% citric acid solution under ultrasonic activation and a final wash with distilled water. All teeth were dried, and 30 specimens were randomly divided into three equal groups as follows: positive control group (PC), no irrigation; 15% citric acid + 2% CHX group (CA + CHX); and 1% NaOCl + 2% CHX group (NaOCl + CHX). All roots were immersed in a 0.2% Rhodamine B solution for 24 hours. One-millimeter-thick slices from the cementum-enamel junction were scanned at 400 dpi and analyzed using the software ImageLab (LIDO-USP, Sao Paulo, Brazil) for the assessment of leakage in percentage. For scanning electron microscopy analysis, four teeth, irrigated for NaOCl + CHX samples, were split in half, and each third was evaluated at 1,000x and 5,000x (at the precipitate). RESULTS Using the analysis of variance test followed by the Bonferroni comparison method, no statistical differences between groups were found when analyzed at the cervical and medium thirds. At the apical third, differences between the PC and NaOCl + CHX (p < 0.05) and CA + CHX and NaOCl + CHX could be seen (p < 0.05). CONCLUSION The combination of 1% NaOCl and 2% CHX solutions results in the formation of a flocculate precipitate that acts as a chemical smear layer reducing the dentinal permeability in the apical third.

Determination of pulp vitality in vivo with pulse oximetry

AIM To evaluate the use of pulse oximetry as a test for pulp vitality, by comparing in the same patient, the levels of oxygen saturation of the index finger and of the maxillary central incisor and canine teeth without clinically detectable pulp inflammation. METHODOLOGY Seventeen male and female patients aged between 26 and 38 years participated and a total of 32 maxillary central incisor and 32 canine teeth were analysed. Selection criteria required the teeth to have healthy crowns, or with restorations no more than 2 mm in diameter and no clinical and radiographical signs or symptoms of pulp or periapical inflammatory changes. The negative control group consisted of 10 root filled teeth. Measurements were first taken from the index finger of patients. Their teeth were then subjected to a thermal test with refrigerant gas and then to a vitality test with pulse oximetry. Data were analysed by Pearsons and paired t-tests. RESULTS There were no significant statistical correlations between blood oxygen levels in the index finger and in the teeth of the patient (P > 0.05). There was a statistically significant difference in the oxygen levels between the two tooth groups studied and the index finger (P <or= 0.002). Mean oxygen values in the index finger of patients were 95% (SD = 1.6), oxygen values in the maxillary central incisor were 91.29% (SD = 2.61) and mean oxygen values in maxillary canine were 90.69% (SD = 2.71). CONCLUSION The method determined consistently the level of blood oxygen saturation of the pulp in maxillary central incisor and canine teeth and can therefore be used for pulp vitality testing. Further studies are required to assess the effectiveness and validity of pulse oximetry in determining pulp vitality in traumatized teeth.

Micropush‐out dentine bond strength of a new gutta‐percha and niobium phosphate glass composite

AIM To characterize an experimental gutta-percha and niobium phosphate glass composite (GNB) applied with a thermoplastic technique to the root canals without sealer in a moist environment and to evaluate its micropush-out bond strength to root canal wall dentine. METHODOLOGY The root canals of sixty human mandibular pre-molars were prepared using rotary NiTi instruments and irrigation with sodium hypochlorite and EDTA. The teeth were then randomly divided into three groups according to the root filling material used: AH plus sealer and gutta-percha (AH), EndoSequence BC gutta-percha without sealer (GBC), and GNB without sealer. The root canals were filled with a single cone using warm vertical condensation. Push-out bond strengths associated with the filling materials in slices from middle root thirds was determined 30 days after root filling. The failure mode was analyzed with SEM. Analysis using EDX and SEM-EDS was carried out to verify the composition and distribution of the particles of the tested materials. Data were statistically analyzed by one-way anova and Tukeys test (P < 0.05). RESULTS AH and GNB groups had bond strengths of 2.83 ± 0.64 MPa and 2.68 ± 0.84 MPa, respectively, with no significant difference between them (P > 0.05). The GBC group had the lowest mean bond strength (1.34 ± 0.42 MPa), which was significantly different compared with the other groups (P < 0.05). Cohesive failures prevailed in the AH group, whereas failures were mixed in the GBC and GNB groups. The SEM-EDS analysis on the surface and in the bulk of GBC revealed only a superficial coating of bioceramic particles. Glass particles were detected both on the surface and in the bulk of GNB. CONCLUSIONS The experimental root filling composite (GNB) had an ability to adhere to root canal wall dentine equal to the current gold standard root filling with gutta-percha and sealer (AH Plus).

Cyclic Fatigue Resistance of Rotary Nickel-Titanium Instruments Submitted to Nitrogen Ion Implantation

INTRODUCTION The aim of this study was to assess cyclic fatigue resistance in rotary nickel-titanium instruments submitted to nitrogen ion implantation by using a custom-made cyclic fatigue testing apparatus. METHODS Thirty K3 files, size #25, taper 0.04, were divided into 3 experimental groups as follows: group A, 12 files exposed to nitrogen ion implantation at a dose of 2.5 x 10(17) ions/cm(2), accelerating voltage of 200 kV, currents of 1 microA/cm(2), 130 degrees C temperature, and vacuum conditions of 10 x 10(-6) torr for 6 hours; group B, 12 nonimplanted files; and group C, 6 files submitted to thermal annealing for 6 hours at 130 degrees C. One extra file was used for process control. All files were submitted to a cyclic fatigue test that was performed with an apparatus that allowed the instruments to rotate freely, simulating rotary instrumentation of a curved canal (40-degree, 5-mm radius curve). An electric motor handpiece was used with a contra-angle of 16:1 at an operating speed of 300 rpm and a torque of 2 N-cm. Time to failure was recorded with a stopwatch in seconds and subsequently converted to number of cycles to fracture. Data were analyzed with the Student t test (P < .05). RESULTS Ion-implanted instruments reached significantly higher cycle numbers before fracture (mean, 510 cycles) when compared with annealed (mean, 428 cycles) and nonimplanted files (mean, 381 cycles). CONCLUSIONS Our results showed that nitrogen ion implantation improves cyclic fatigue resistance in rotary nickel-titanium instruments. Industrial implementation of this surface modification technique would produce rotary nickel-titanium instruments with a longer working life.

A micro-computed tomography evaluation of long-oval canal preparation using reciprocating or rotary systems.

AIM To evaluate, using micro-computed tomography, the preparation of long-oval root canals using a single reciprocating system versus a multiple-file rotary system. METHODOLOGY Distal canals of thirty mandibular molars were selected and randomly assigned to one of two instrument groups (n = 15): Reciproc 40 (VDW, Munich, Germany) or BioRaCe system (FKG Dentaire, La Chaux-de-Fonds, Switzerland). The teeth were scanned before and after preparation of the canal by a SkyScan 1172 micro-computed tomography scanner at 11-μm resolution. Morphometric variations were measured by volume increases and by the remaining untreated canal surface area in the entire canal and as well as in each third of the canal. Data were compared using the Mann-Whitney test. RESULTS The Reciproc system left significantly more areas untouched (P < 0.001) in the cervical and middle thirds (18.14% and 21.82%) as compared to BioRaCe (8.14% and 11.35%). The Reciproc system had the greatest increase in volume of both the entire canal and the apical third (P < 0.5). CONCLUSIONS Neither technique was able to completely prepare the outline of long-oval canals. The Reciproc system removed more tooth structure. The BioRaCe left fewer untouched dentine walls in the more coronal thirds of the canal, whilst Reciproc left fewer in the apical third.

Apical Root Canal Transportation and Remaining Dentin Thickness Associated with ProTaper Universal with and without PathFile

INTRODUCTION This study aimed to compare, using digital subtraction radiography, apical transportation in mesiobuccal root canals of extracted human maxillary molars instrumented with ProTaper Universal F3 and F4 files (Dentsply Maillefer, Ballaigues, Switzerland) with and without the prior creation of glide paths using the PathFile system and to compare, using cone-beam computed tomographic imaging, remaining dentin thickness (RDT) after complete preparation. METHODS Forty mesiobuccal roots with curvatures of 20°-35° were used. In group A, glide paths were created with PathFile #1, #2, and #3 to the working length; in group B, no glide paths were used. All canals were instrumented up to F4 to the working length. Digital subtraction radiographic images were obtained in buccolingual and mesiodistal directions. Apical transportation associated with F3 or F4 was calculated based on the difference between the tip of the first and the last instrument analyzed in each group. RDT was calculated based on cone-beam computed tomographic images. RESULTS Apical transportation was higher after F4 regardless of the use of the PathFile system. No statistically significant differences between groups A and B were observed in apical transportation after instrumentation with F3 or F4 (P > .05). Group A showed greater RDT values at 2 (buccal and mesial walls, P < .05) and 3 mm (buccal and distal walls, P < .05) from the apex but not at 1 mm. CONCLUSIONS The use of the PathFile system before root canal preparation with ProTaper Universal F3 and F4 did not influence apical transportation but was associated with greater RDTs at 2 and 3 mm from the apex.

Microscopic computerized tomographic evaluation of root canal transportation prepared with twisted or ground nickel-titanium rotary instruments

OBJECTIVE The aim of this study was to evaluate, ex vivo, canal transportation and the centering ability of nickel-titanium rotary instruments manufactured by twisting and by traditional grinding, with the use of microscopic computerized tomography (μCT). STUDY DESIGN Fifteen mandibular molars were embedded in a rubber-based impression material and submitted to μCT before and after instrumentation. Images were reconstructed, and cross-sections corresponding to distances 1, 2, 3, 4, 5, 6, and 7 mm from the anatomic apex were selected for analysis. Statistical analysis was performed with Mann-Whitney test. RESULTS Canal transportation and centering ability results were similar for both instruments. Statistically significant differences (P < .05) were observed only at the 3 and 4 mm cross-sections, with lower levels of apical transportation and a better centering ratio associated with twisted instruments than with ground instruments. CONCLUSIONS Our findings showed that twisted and ground instruments behaved similarly, allowing the preparation of curved canals with little transportation, which occurred in both mesial and distal directions.

Influence of dentin on pH of 2% chlorhexidine gel and calcium hydroxide alone or in combination

The aims of endodontic treatment in cases of apical periodontitis are to reduce as much as possible the number of microorganisms inside the root canal system and to inactivate toxins produced by them. Most of the times, these objectives are not achieved solely by chemomechanical preparation, and intracanal dressing may be necessary. In these cases, calcium hydroxide is used as a root canal dressing due to its well-known and recognized antimicrobial activity. Chlorhexidine has a wide spectrum of antimicrobial activity and its association with calcium hydroxide has been recommended in an attempt to amplify antimicrobial effects of calcium hydroxide. It is also known that dentin exerts a buffering effect under wide pH variations, and may be responsible for decreasing the antimicrobial activity of drugs inside the root canal. The objectives of this study were to assess the pH of 2% chlorhexidine gel and calcium hydroxide alone or in combination, as well as the influence of dentin on the pH of these compounds. Dentin powder was obtained from bovine teeth and added as 1.8% to the volume of the medications. All substances were individually stored in plastic flasks, in triplicate. A pH meter was used at five different moments to assess pH in viscous medium: immediately after preparation and after 24 h, and 7, 14, and 21 days. Results were analyzed by paired Students t-test. Statistically significant differences were observed in the 2% chlorhexidine gel group alone or associated with calcium hydroxide and added of dentin powder (P < 0.05). Mean pH values indicated the influence of dentin powder because of a significant increase in pH. Calcium hydroxide with propylene glycol as the vehicle always showed high pH, demonstrating that this compound was not affected by the presence of dentin.