Giuseppe Morabito

Acceleration of cyanobacterial dominance in north temperate‐subarctic lakes during the Anthropocene

Increases in atmospheric temperature and nutrients from land are thought to be promoting the expansion of harmful cyanobacteria in lakes worldwide, yet to date there has been no quantitative synthesis of long-term trends. To test whether cyanobacteria have increased in abundance over the past ~ 200 years and evaluate the relative influence of potential causal mechanisms, we synthesised 108 highly resolved sedimentary time series and 18 decadal-scale monitoring records from north temperate-subarctic lakes. We demonstrate that: (1) cyanobacteria have increased significantly since c. 1800 ce, (2) they have increased disproportionately relative to other phytoplankton, and (3) cyanobacteria increased more rapidly post c. 1945 ce. Variation among lakes in the rates of increase was explained best by nutrient concentration (phosphorus and nitrogen), and temperature was of secondary importance. Although cyanobacterial biomass has declined in some managed lakes with reduced nutrient influx, the larger spatio-temporal scale of sedimentary records show continued increases in cyanobacteria throughout the north temperate-subarctic regions.

Assessing remotely sensed chlorophyll-a for the implementation of the Water Framework Directive in European perialpine lakes

The lakes of the European perialpine region constitute a large water reservoir, which is threatened by the anthropogenic pressure altering water quality. The Water Framework Directive of the European Commission aims to protect water resources and monitoring is seen as an essential step for achieving this goal. Remote sensing can provide frequent data for large scale studies of water quality parameters such as chlorophyll-a (chl-a). In this work we use a dataset of maps of chl-a derived from over 200 MERIS (MEdium Resolution Imaging Spectrometer) satellite images for comparing water quality of 12 perialpine lakes in the period 2003-2009. Besides the different trophic levels of the lakes, results confirm that the seasonal variability of chl-a concentration is particularly pronounced during spring and autumn especially for the more eutrophic lakes. We show that relying on only one sample for the assessment of lake water quality during the season might lead to misleading results and erroneous assignments to quality classes. Time series MERIS data represents a suitable and cost-effective technology to fill this gap, depicting the dynamics of the surface waters of lakes in agreement with the evolution of natural phenomena.

Ecological status assessment of European lakes: a comparison of metrics for phytoplankton, macrophytes, benthic invertebrates and fish

Data on phytoplankton, macrophytes, benthic invertebrates and fish from more than 2000 lakes in 22 European countries were used to develop and test metrics for assessing the ecological status of European lakes as required by the Water Framework Directive. The strongest and most sensitive of the 11 metrics responding to eutrophication pressure were phytoplankton chlorophyll a, a taxonomic composition trophic index and a functional traits index, the macrophyte intercalibration taxonomic composition metric and a Nordic lake fish index. Intermediate response was found for a cyanobacterial bloom intensity index (Cyano), the Ellenberg macrophyte index and a multimetric index for benthic invertebrates. The latter also responded to hydromorphological pressure. The metrics provide information on primary and secondary impacts of eutrophication in the pelagic and the littoral zone of lakes. Several of these metrics were used as common metrics in the intercalibration of national assessment systems or have been incorporated directly into the national systems. New biological metrics have been developed to assess hydromorphological pressures, based on aquatic macrophyte responses to water level fluctuations, and on macroinvertebrate responses to morphological modifications of lake shorelines. These metrics thus enable the quantification of biological impacts of hydromorphological pressures in lakes.

Trophic development of the deep lakes south of the Alps: a comparative analysis

This paper reports the results of long-term research carried out in the deep southern subalpine lakes (DSL: Maggiore, Lugano, Como, Iseo and Garda). The measurements performed since the 1970s at the time of the maximum overturn documented an increase of water temperature around 0.012-0.028 °C yc -1 , closely resembling the warming rate found in other lakes in Europe and North America. The DSL showed an increase of total phosphorus after the 1960s and a complete recovery after the 1990s of Lake Maggiore, which reached mean values of TP close to those of the pristine conditions (<10μgPl -1 Present TP concentrations in the other lakes are above 100 μg P l -1 (Lugano), 30-40 μg P l -1 (Como), 50-60 μg P l -1 (Iseo) and c. 20 μg P l -1 (Garda), with only Lake Garda showing a clear tendency to increasing P-concentrations in recent years. The higher trophic status of lakes Lugano and Iseo has favoured hypolimnetic anoxia due to meromixis since the 1960s and the 1990s, respectively, from which there was a partial recovery in 2005. Monthly samplings carried out in the last decades showed a strong coupling between long-term variation in algal nutrients and phytoplankton. On a shorter temporal scale, a significant relationship between the extent of water mixing during the spring months and the replenishment of nutrients and algal biomass in the growing season has also been verified in lakes Iseo, Garda and Lugano.

Trends and relations among basic phytoplankton characteristics in the course of the long-term oligotrophication of Lake Maggiore (Italy)

Lake Maggiore underwent a process of eutrophication in the course of the 1960s and 1970s which caused the lake status to change from oligotrophy to meso-eutrophy, sewage phosphorus being the main responsible factor. Starting from the late 1970s the P load has been gradually reduced, so that total phosphorus in-lake concentration is now below 10 µg l-1 at winter mixing, compared to a maximum value of 30 µg l-1 in 1978. Nearly two decades after the start of this reversal in trophic conditions, long-term analyses become fruitful, not least because of a scarcity of published long-term data on lake re-oligotrophication, and consequently of agreed predictions on specific points. This paper presents such an analysis, regarding some phytoplankton characteristics (numbers, biovolume, cell size, chlorophyll a, species richness and composition) and their mutual relations in the time series 1981-1995, as well as recent primary production compared with early observations. Notwithstanding phosphorus reduction, the phytoplankton remained quite stable for many years. Only after 1987, when TP concentration fell to about 15 µg l-1 at mixing, definite changes in species diversity and composition appeared. At the same time, average cell size decreased abruptly and persistently, together with chlorophyll and total biovolume while cell numbers were unchanged or rising. Annual primary production was halved in comparison to a past reference period and the efficiency of the photosynthetic process was apparently enhanced.

Strength and uncertainty of phytoplankton metrics for assessing eutrophication impacts in lakes

Phytoplankton constitutes a diverse array of short-lived organisms which derive their nutrients from the water column of lakes. These features make this community the most direct and earliest indicator of the impacts of changing nutrient conditions on lake ecosystems. It also makes them particularly suitable for measuring the success of restoration measures following reductions in nutrient loads. This paper integrates a large volume of work on a number of measures, or metrics, developed for using phytoplankton to assess the ecological status of European lakes, as required for the Water Framework Directive. It assesses the indicator strength of these metrics, specifically in relation to representing the impacts of eutrophication. It also examines how these measures vary naturally at different locations within a lake, as well as between lakes, and how much variability is associated with different replicate samples, different months within a year and between years. On the basis of this analysis, three of the strongest metrics (chlorophyll-a, phytoplankton trophic index (PTI), and cyanobacterial biovolume) are recommended for use as robust measures for assessing the ecological quality of lakes in relation to nutrient-enrichment pressures and a minimum recommended sampling frequency is provided for these three metrics.

Phytoplankton as an Indicator of the Water Quality of the Deep Lakes South of the Alps

This paper offers a synoptic account of studies on the phytoplankton communities in the deep southern subalpine lakes (DSL) Garda, Iseo, Como, Lugano and Maggiore. The main cause of the degradation of the water quality in the DSL is eutrophication. The euphotic layers of these lakes are trophically different, ranging from the oligo-mesotrophy of lakes Maggiore and Garda to the meso-eutrophy of lakes Iseo and Lugano. The trophic status as estimated by using total phosphorus and chlorophyll a has provided consistent results in agreement with the models proposed by OECD (1982. Eutrophication of Waters. Monitoring, Assessment and Control, OECD, Paris). Though related with chlorophyll a and TP, the Secchi disk depths have significantly underestimated the trophic status of the DSL. Two trophic indices using the algal orders (PTIorders) and species (PTIspecies) were drawn up on the basis of the distribution of phytoplankton along a trophic gradient defined by the application of multivariate methods; the scores emerging from these indices were used to make a definitive ecological classification of water bodies on a scale from 1 to 5, in accordance with the Water Framework Directive. A third index (PTIOE) was computed as the ratio between the annual mean values of the cumulative biovolumes of two groups of algal orders with opposite trophic characteristics. The three PTI indices were highly correlated, providing a consistent classification of the water bodies. The indices proposed in this work were specifically adopted for use in the DSL. However, the criteria for their implementation constitute a robust and impartial tool for assessing similar indices in other lake typologies and for evaluating the degree of specificity of the trophic indicator values assigned to the single phytoplankton orders and species.

A phytoplankton trophic index to assess the status of lakes for the Water Framework Directive

Despite improvements in wastewater treatment systems, the impact of anthropogenic nutrient sources remains a key issue for the management of European lakes. The Water Framework Directive (WFD) provides a mechanism through which progress can be made on this issue. The Directive requires a classification of the ecological status of phytoplankton, which includes an assessment of taxonomic composition. In this paper, we present a composition metric, the plankton trophic index, that was developed in the WISER EU FP7 project and demonstrate how it has been used to compare national phytoplankton classification systems in Northern and Central Europe. The metric was derived from summer phytoplankton data summarised by genus from 1,795 lakes, covering 20 European countries. We show that it is significantly related to total phosphorus concentrations, but that it is also sensitive to alkalinity, lake size and climatic variables. Through the use of country-specific reference values for the index, we demonstrate that it is significantly related to other national phytoplankton assessment systems and illustrate for a single European (intercalibration) lake type how it was used to intercalibrate WFD boundaries from different countries.

Effects of nutrient availability and temperature on phytoplankton development: a case study from large lakes south of the Alps

This work investigated the combined effects of nutrient availability and temperature on phytoplankton in large and deep lakes south of the Alps (lakes Garda, Iseo, Como, Lugano and Maggiore). The more eutrophic basins (Lugano and Iseo) showed a higher presence of cyanobacteria, green algae (Chlorophyta and Charophyta) and dinoflagellates (Dinophyta). Besides these two water bodies, high biomasses of cyanobacteria were recorded also in the oligo-mesotrophic Lake Garda. The development of these algal groups during the growing season showed a strong dependence on the surface spring availability of SRP, which, in turn, was related to winter climatic oscillations, deep mixing dynamics, and trophic status. A specific analysis carried out by applying additive mixed modelling, generalized least squares and mixed modelling, allowed investigation of the direct, seasonal effects of water temperature variations and trophic status on different algal groups. The dominant cyanobacteria (Oscillatoriales) showed only a partial relationship with temperature, while Nostocales and Chroococcales, which did not appear to have a close relationship with the trophic status of the lakes, were characterised by abrupt increases during the warmer months. High positive relationships with temperature were found for a few other algal groups (e.g., Chlorophyta, Charophyta and Dinophyta). Overall, the results indicated a positive relationship between the seasonal development of the more abundant and eutrophic-sensitive algal groups and the concurrent effect of trophic status and water temperature. Nevertheless, it was stressed that specific differences could be interpreted taking into account the different autoecological characteristics and susceptibilities of different species and functional groups to other stressing factors favouring losses, including, e.g., vertical sinking and grazing.

Phytoplankton assemblage at equilibrium in large and deep subalpine lakes: a case study from Lago Maggiore (N. Italy)

In 1996, we studied the phytoplankton seasonal succession in Lago Maggiore (N. Italy) through weekly sampling. Such a frequency enabled us to evaluate the changes of the phytoplankton assemblage in the light of the equilibrium and non-equilibrium theories. The distinct phases of changing of the species composition were identified separating the samples by means of cluster analysis and non-metric multidimensional scaling (NMDS). We recognised well distinct phytoplankton associations, whose seasonal succession followed a clear cyclic path throughout the year, with spring and summer phases respectively characterised by a rapid turn-over of the assemblages and by a relative stability. Moreover, we observed an increase of species number and Shannon-Wiener diversity during the spring, followed by a summer decline of the diversity in spite of an unchanged species number. Because of the dominance of the same few species for about two months during summer, coupled with small fluctuations of the total biomass, we could identify the summer assemblage as a steady state assemblage. The aim of the present contribution, although describing the whole seasonal succession, is to draw the attention towards the species composition at the steady state, taking into account the functional properties of the species involved. The possible role of the metalimnetic niche in selecting a particular summer assemblage in deep and large lakes will be discussed.