Giuseppe Palumbo

Autofluorescence spectroscopy of cells and tissue as a tool for biomedical diagnosis

Several biomolecules involved both in the metabolic processes and in the histological organization of cells and tissues are characterized by fluorescence properties that can be exploited to obtain information on the morpho-functional conditions of the biological substrate, suitable for diagnostic applications. An overview of the endogenous fluorophores responsible for the autofluorescence is given concerning the photophysical properties and their dependence on the evolution of the biological condition of cells and tissues.

Lasers and current optical techniques in biology

Part I: Lasers and Lamps Gas State Lasers Liquid State Lasers Solid State Lasers Semiconductor Lasers Diode Pumped Solid State Lasers Incoherent Light Sources Solid State Lamps Fibre Lasers Methods for the Generation of Light Pulses: From Milliseconds to Attoseconds Part II: Spectroscopic and Imaging Techniques The Autofluorescence Spectroscopy of Cells and Tissue as a Tool for Biomedical Diagnosis Reflectance and Transmittance Spectroscopy Fluorescence Spectroscopy Part III: Microscopy Techniques Optical Microscopy Wide-Field Autofluorescence Microscopy for Imaging of Living Cell Scanning Probe Microscopy Confocal and Multiphoton Microscopy Part IV: Advancing Imaging Techniques and Novel Ultrasensitive Fluorescence Detection Techniques Optical Coherence Tomography Laser Optoacoustic Imaging Polarized Light Imaging Ultrasensitive Fluorescence Detection at Surfaces: Instrument Development, Surface Chemistry, and Applications in Life Science and Medicine Subject Index.

Polarized light imaging of tissues

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Part IV: Advancing imaging techniques and novel ultrasensitive fluorescence detection techniques

Part IV Advanced Imaging Techniques and Novel Ultrasensitive Fluorescence Detection Techniques