Glenn Albrecht
Molecular Sciences Institute
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Publication
Featured researches published by Glenn Albrecht.
Nature Biotechnology | 2000
Sydney Brenner; Maria Johnson; John Bridgham; George Golda; David H. Lloyd; Davida Johnson; Shujun Luo; Sarah N. McCurdy; Michael F. Foy; Mark Ewan; Rithy Roth; Dave George; Sam Eletr; Glenn Albrecht; Eric Vermaas; Steven R. Williams; Keith Moon; Timothy Burcham; Michael C. Pallas; Robert B. Dubridge; James J. Kirchner; Karen L. Fearon; Jen-I Mao; Kevin Corcoran
We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 μm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing microbeads in a flow cell at a density greater than 3 × 106 microbeads/cm2. Sequences of the free ends of the cloned templates on each microbead were then simultaneously analyzed using a fluorescence-based signature sequencing method that does not require DNA fragment separation. Signature sequences of 16–20 bases were obtained by repeated cycles of enzymatic cleavage with a type IIs restriction endonuclease, adaptor ligation, and sequence interrogation by encoded hybridization probes. The approach was validated by sequencing over 269,000 signatures from two cDNA libraries constructed from a fully sequenced strain of Saccharomyces cerevisiae, and by measuring gene expression levels in the human cell line THP-1. The approach provides an unprecedented depth of analysis permitting application of powerful statistical techniques for discovery of functional relationships among genes, whether known or unknown beforehand, or whether expressed at high or very low levels.
Proceedings of the National Academy of Sciences of the United States of America | 2000
Sydney Brenner; Steven R. Williams; Eric Vermaas; Thorsten Storck; Keith Moon; Christie McCollum; Jen-I Mao; Shujun Luo; James J. Kirchner; Sam Eletr; Robert B. Dubridge; Timothy Burcham; Glenn Albrecht
Archive | 1995
Linda C. Griffin; Glenn Albrecht; John Latham; Lawrence L. K. Leung; Eric Vermaas; John J. Toole
Archive | 1997
Glenn Albrecht; Sydney Brenner; Robert B. Dubridge; David H. Lloyd; Michael C. Pallas
Archive | 1997
Robert B. Dubridge; Glenn Albrecht; Sydney Brenner; Sergei M. Gryaznov; Sarah N. McCurdy
Archive | 1998
Sydney Brenner; Glenn Albrecht; Stephen C. Macevicz
Archive | 1997
Glenn Albrecht; Sydney Brenner; David H. Lloyd; Robert B. Dubridge; Michael C. Pallas
Archive | 1999
Glenn Albrecht; Sydney Brenner; Robert B. Dubridge
Archive | 2001
Rongdian Fu; Sydney Brenner; Glenn Albrecht
Archive | 2003
Shujun Luo; Martin Kuiper; Jen-I Mao; Glenn Albrecht; Marc Cornelissen