Glenn Hoke

Synthesis and Biophysical and Biological Evaluation of 2′-Modified Antisense Oligonucleotides

Abstract A series of 2′-deoxy-2′-substituted adenosine modified oligonucleotides were synthesized and evaluated for their thermodynamic stability (Tms) and resistance to nuclease degradation in fetal calf serum.

Synthesis and Biological Evaluation of Antisense Oligonucleotides Containing Modified Pyrimidines

Abstract Antisense oligodeoxynucleotides (ODNs) containing certain pyrimidines modified at the 5 and/or 6-position(s) of the heterocycle were synthesized. The ODNs were evaluated for their resistance to nuclease degradation in serum, hybridization properties, and ability to activate RNase H.

Inhibition of 5-α-Reductase (TYPE-II) Expression by Antisense 3′-Deoxy-(2′-5′) Oligonucleotide Chimeras

Abstract ABSTRACT: 3′-Deoxy-(2′-5′) oligonucleotides bind selectively to complementary RNA but not to DNA. 3′-Deoxy-(2′-5′) phosphorothioate ODN chimeras embedded with a short stretch of 3′-5′ phosphorothioate cassette are potent inhibitors of steroid 5-α-reductasc expression with significantly less non-specific interactions in cell culture.