Gonçalo C. Rodrigues

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Featured researches published by Gonçalo C. Rodrigues.

Methods of Molecular Biology | 2014

Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting

Gonçalo C. Rodrigues; Tiago G. Fernandes; Carlos A. V. Rodrigues; J. M. S. Cabral; Maria Margarida Diogo

Neural precursor (NP) cells derived from human induced pluripotent stem cells (hiPSCs), and their neuronal progeny, will play an important role in disease modeling, drug screening tests, central nervous system development studies, and may even become valuable for regenerative medicine treatments. Nonetheless, it is challenging to obtain homogeneous and synchronously differentiated NP populations from hiPSCs, and after neural commitment many pluripotent stem cells remain in the differentiated cultures. Here, we describe an efficient and simple protocol to differentiate hiPSC-derived NPs in 12 days, and we include a final purification stage where Tra-1-60+ pluripotent stem cells (PSCs) are removed using magnetic activated cell sorting (MACS), leaving the NP population nearly free of PSCs.

Agricultural Water Management | 2012

Implementing the dual crop coefficient approach in interactive software. 1. Background and computational strategy

Ricardo D. Rosa; Paula Paredes; Gonçalo C. Rodrigues; Isabel Alves; R.M. Fernando; Luis S. Pereira; Richard G. Allen

Biosystems Engineering | 2009

Assessing economic impacts of deficit irrigation as related to water productivity and water costs

Gonçalo C. Rodrigues; Luis S. Pereira

Irrigation Science | 2012

The dual crop coefficient approach using a density factor to simulate the evapotranspiration of a peach orchard: SIMDualKc model versus eddy covariance measurements

T.A. Paço; M. I. Ferreira; R.D. Rosa; Paula Paredes; Gonçalo C. Rodrigues; N. Conceição; Carlos Arruda Pacheco; Luis S. Pereira

Agricultural Water Management | 2014

Partitioning evapotranspiration, yield prediction and economic returns of maize under various irrigation management strategies

Paula Paredes; Gonçalo C. Rodrigues; I. Alves; Luis S. Pereira

Agricultural Water Management | 2012

Implementing the dual crop coefficient approach in interactive software. 2. Model testing

Ricardo D. Rosa; Paula Paredes; Gonçalo C. Rodrigues; R.M. Fernando; Isabel Alves; Luis S. Pereira; Richard G. Allen

Biosystems Engineering | 2013

Dual crop coefficients for maize in southern Brazil: Model testing for sprinkler and drip irrigation and mulched soil

Juliano Dalcin Martins; Gonçalo C. Rodrigues; Paula Paredes; Reimar Carlesso; Zanandra Boff de Oliveira; Alberto Eduardo Knies; Mirta Teresinha Petry; Luis S. Pereira

Agricultural Water Management | 2013