Gonzalo Muyo

Spectral imaging of the retina

IntroductionThe work described here involved the use of a modified fundus camera to obtain sequential hyperspectral images of the retina in 14 normal volunteers and in 1 illustrative patient with a retinal vascular occlusion.MethodsThe paper describes analysis techniques, which allow oximetry within retinal vessels; these results are presented as retinal oximetry maps.ResultsUsing spectral images, with wavelengths between 556 and 650 nm, the mean oxygen saturation (OS) value in temporal retinal arterioles in normal volunteers was 104.3 (±16.7), and in normal temporal retinal venules was 34.8 (±17.8). These values are comparable to those quoted in the literature, although, the venular saturations are slightly lower than those values found by other authors; explanations are offered for these differences.DiscussionThe described imaging and analysis techniques produce a clinically useful map of retinal oximetric values. The results from normal volunteers and from one illustrative patient are presented. Further developments, including the recent development of a ‘snapshot’ spectral camera, promises enhanced non-invasive retinal vessel oximetry mapping.

Infrared imaging with a wavefront-coded singlet lens

We describe the use of wavefront coding for the mitigation of optical aberrations in a thermal imaging system. Diffraction-limited imaging is demonstrated with a simple singlet which enables an approximate halving in length and mass of the optical system compared to an equivalent two-element lens.

Decomposition of the optical transfer function: wavefront coding imaging systems

We describe the mapping of the optical transfer function (OTF) of an incoherent imaging system into a geometrical representation. We show that for defocused traditional and wavefront-coded systems the OTF can be represented as a generalized Cornu spiral. This representation provides a physical insight into the way in which wavefront coding can increase the depth of field of an imaging system and permits analytical quantification of salient OTF parameters, such as the depth of focus, the location of nulls, and amplitude and phase modulation of the wavefront-coding OTF.

Circularly symmetric phase filters for control of primary third-order aberrations: coma and astigmatism

A quartic phase retardation function is described that reduces the variation of the intensity of the focal point of incoherent imaging systems suffering from primary third-order aberrations limited to coma and astigmatism. Corresponding modulation transfer functions are shown to remain practically invariant for moderate amounts of coma and astigmatism.

Validation of Human Whole Blood Oximetry, Using a Hyperspectral Fundus Camera with a Model Eye

PURPOSE To assess the accuracy of human blood oximetry measurements in a model eye with a hyperspectral fundus camera. METHODS Seven human whole blood samples (two arterial, five venous) were obtained, the oxygen saturations measured with a CO oximeter, and the samples inserted into quartz tubes with internal diameters of 100 and 150 μm. The tubes (n = 20; ten 100 μm and ten 150 μm) were placed within a model eye in front of a background reflectance surface with reflectivities of 20%, 60%, and 99%. Spectral images at wavelengths between 500 and 650 nm were acquired with a hyperspectral fundus camera and analyzed with an oximetric model to calculate the oxygen saturation of blood within the tubes. The calculated oxygen saturations were compared with the measured oxygen saturations. The effects of the background reflectivity and tube size on the accuracy of the calculated oxygen saturations were evaluated. RESULTS Background reflectivity and tube size had no significant effect on the mean oxygen saturation difference (P = 0.18 and P = 0.99, respectively; repeated-measures, two-way ANOVA). The mean differences (SD) between the measured and calculated oxygen saturations in segments of the 100 and 150 μm tubes overlying the 20%, 60%, and 99% background reflectivities were (100 μm) -4.0% (13.4%), -6.4% (9.9%), and -5.5% (10.2%) and (150 μm) -5.3% (10.8%), -5.2% (10.7%), and -5.2% (10.9%), respectively. CONCLUSIONS There was reasonable agreement between the measured oxygen saturation values and those calculated by the oximetry model. The oximetry model could be used to determine the functional health of the retina.

Wavefront coding for athermalization of infrared imaging systems

Wavefront coding involves the insertion of an asymmetric refractive mask close to the pupil plane of an imaging system so as to encode the image with a specific point spread function that, when combined with decoding of the recorded image, can enable greatly reduced sensitivity to imaging aberrations. The application of wavefront coding has potential in the fields of microscopy, where increased instantaneous depth of field is advantageous and in thermal imaging where it can enable the use of simple, low-cost, light-weight lens systems. It has been previously shown that wavefront coding can alleviate optical aberrations and extend the depth of field of incoherent imaging systems whilst maintaining diffraction-limited resolution. It is particularly useful in controlling thermally induced defocus aberrations in infrared imaging systems. These improvements in performance are subject to a range of constraints including the difficulty in manufacturing an asymmetrical phase mask and significant noise amplification in the digitally restored image. We describe the relation between the optical path difference (OPD) introduced by the phase mask and the magnitude of noise amplification in the restored image. In particular there is a trade between the increased tolerance to optical aberrations and reduced signal-to-noise ratio in the recovered image. We present numerical and experimental studies based of noise amplification with the specific consideration of a simple refractive infrared imaging system operated in an ambient temperature varying from 0°C to +50 C. These results are used to delineate the design and application envelope for which infrared imaging can benefit from wavefront coding.

Oxygen saturation measurements of the retinal vasculature in treated asymmetrical primary open-angle glaucoma using hyperspectral imaging

PurposeTo determine whether there are differences in retinal vascular oxygen saturation measurements, estimated using a hyperspectral fundus camera, between normal eyes and treated eyes of subjects with asymmetrical primary open-angle glaucoma (POAG).MethodsA noninvasive hyperspectral fundus camera was used to acquire spectral images of the retina at wavelengths between 556 and 650 nm in 2-nm increments. In total, 14 normal eyes and both eyes of 11 treated POAG subjects were imaged and analyzed using algorithms that use the spectral variation of the optical densities of blood vessels to estimate the oxygen saturation of blood within the retinal vasculature. In the treated POAG group, each of the eyes were categorized, based on the mean deviation of the Humphrey visual-field analyzer result, as either more-advanced or less-advanced, glaucomatous eyes. Unpaired t-tests (two-tailed) with Welch’s correction were used to compare the mean oxygen saturation between the normal subjects and the treated POAG subgroups.ResultsIn less-advanced and more-advanced-treated POAG eyes, mean retinal venular oxygen saturations (48.2±21.6% and 42.6±18.8%, respectively) were significantly higher than in normal eyes (27.9±9.9%; P=0.03 and 0.01, respectively). Arteriolar oxygen saturation was not significantly different between normal eyes and treated POAG eyes.ConclusionsThe increased oxygen saturation of the retinal venules in advanced-treated POAG eyes may indicate reduced metabolic consumption of oxygen in the inner retinal tissues.

Amplitude and phase filters for mitigation of defocus and third-order aberrations

This paper gives a review on the design and use of both amplitude filters and phase filters to achieve a large focal depth in incoherent imaging systems. Traditional optical system design enhances the resolution of incoherent imaging systems by optical-only manipulations or some type of post-processing of an image that has been already recorded. A brief introduction to recent techniques to increase the depth of field by use of hybrid optical/digital imaging system is reported and its performance is compared with a conventional optical system. This technique, commonly named wavefront coding, employs an aspherical pupil plane element to encode the incident wavefront in such a way that the image recorded by the detector can be accurately restored over a large range of defocus. As reported in earlier work, this approach alleviates the effects of defocus and its related aberrations whilst maintaining diffraction-limited resolution. We explore the control of third order aberrations (spherical aberration, coma, astigmatism, and Petzval field curvature) through wavefront coding. This method offers the potential to implement diffraction-limited imaging systems using simple and low-cost lenses. Although these performances are associated with reductions in signal-to-noise ratio of the displayed image, the jointly optimized optical/digital hybrid imaging system can meet some specific requirements that are impossible to achieve with a traditional approach.

New spectral imaging techniques for blood oximetry in the retina

Hyperspectral imaging of the retina presents a unique opportunity for direct and quantitative mapping of retinal biochemistry - particularly of the vasculature where blood oximetry is enabled by the strong variation of absorption spectra with oxygenation. This is particularly pertinent both to research and to clinical investigation and diagnosis of retinal diseases such as diabetes, glaucoma and age-related macular degeneration. The optimal exploitation of hyperspectral imaging however, presents a set of challenging problems, including; the poorly characterised and controlled optical environment of structures within the retina to be imaged; the erratic motion of the eye ball; and the compounding effects of the optical sensitivity of the retina and the low numerical aperture of the eye. We have developed two spectral imaging techniques to address these issues. We describe first a system in which a liquid crystal tuneable filter is integrated into the illumination system of a conventional fundus camera to enable time-sequential, random access recording of narrow-band spectral images. Image processing techniques are described to eradicate the artefacts that may be introduced by time-sequential imaging. In addition we describe a unique snapshot spectral imaging technique dubbed IRIS that employs polarising interferometry and Wollaston prism beam splitters to simultaneously replicate and spectrally filter images of the retina into multiple spectral bands onto a single detector array. Results of early clinical trials acquired with these two techniques together with a physical model which enables oximetry map are reported.

The effect of detector sampling in wavefront-coded imaging systems

We present a comprehensive study of the effect of detector sampling on wavefront-coded systems. Two important results are obtained: the spurious response ratios are reduced in wavefront-coded systems with a cubic phase mask and detector sampling does not compromise the restoration of wavefront-coded images with extended depth of field. Rigorous computer simulation of sampled wavefront-coded images shows an increased signal-to-aliased-noise ratio of up to 16% for a cubic phase mask with α = 5λ.