Gottfried Grabner
Max F. Perutz Laboratories
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Publication
Featured researches published by Gottfried Grabner.
Journal of Cell Science | 2005
Christina Maier; Dominik Rünzler; Julia Schindelar; Gottfried Grabner; Werner Waldhäusl; Gottfried Köhler; Anton Luger
Rapid, nongenomic actions of glucocorticoids (GCs) have been well documented, but information about putative membrane receptors that mediate them is scarce. We used fluorescence correlation spectroscopy to search for membrane GC-binding on the mouse pituitary cell line AtT-20. A slowly diffusing fraction (τ3; diffusion constant 3×10-10 cm2 s-1) of fluorescein-labeled dexamethasone on the cell membrane corresponds to fluorescein-dexamethasone binding. Preincubation experiments were performed to test binding specificity: a 500-fold excess of unlabeled dexamethasone abolished subsequent fluorescein-dexamethasone membrane binding from 58±2 (control) to 8±1 (% of τ3, mean ± s.e.m.), the natural ligand corticosterone prevented it partially (29±2), while the sex steroids estradiol (56±4) and progesterone (50±4) and the GC-receptor antagonist RU486 (56±2) had no effect. Preincubation with pertussis toxin resulted in disappearance of the slowest diffusion component (11±4) suggesting association of the receptor with a G-protein. Varying the concentration of fluorescein-dexamethasone showed that membrane binding is highly cooperative with an apparent Kd of 180 nM and Bmax of 230 nM. Taken together, these results demonstrate high-affinity GC-binding on the cell membrane of AtT-20 cells with characteristics distinct from intracellular binding.
Macromolecules | 2012
Markus Griesser; Arnulf Rosspeintner; Claudia Dworak; Michael Hofer; Gottfried Grabner; Robert Liska; Georg Gescheidt
Typical bimolecular photoinitiators (PIs) for radical polymerization of acrylates show only poor photoreactivity because of the undesired effect of back electron transfer. To overcome this limitation, PIs consisting of a benzaldoxime ester and various sensitizers based on aromatic ketones were introduced. The core of the photoinduced reactivity was established by laser flash photolysis, photo-CIDNP, and EPR experiments at short time scales. According to these results, the primarily formed iminyl radicals are not particularly active. The polymerization is predominantly initiated by C-centered radicals. Photo-DSC experiments show reactivities comparable to that of classical monomolecular type I PIs like Darocur 1173.
Journal of Photochemistry and Photobiology B-biology | 2008
Sameh R. El-Gogary; Gottfried Grabner; Gottfried Köhler
Intersystem crossing quantum yields of two psoralen derivatives, bromopsoralen (BrMOP) and hexylpsoralen (8-HOP) were measured in ethanol and water by means of laser flash photolysis. Compared to 8-methoxypsoralen (8-MOP), the triplet quantum yields of BrMOP and 8-HOP in ethanol are increased by a factor of 5 and 30, respectively, while BrMOP in aqueous solution shows a twofold enhancement with respect to 8-MOP. Radical cations and hydrated electrons were generated by photoionization in micellar solution upon excitation at 266nm. A nonlinear relationship between transient yield and photon fluence was obtained for each compound, indicating that a two-photon mechanism is predominant in the photoionization of the sensitizers. The photoionization efficiencies are significantly higher in anionic sodium dodecyl sulfate (SDS) than in cationic cetyltrimethylammonium bromide (CTAB) micelles, reflecting the influence of micelle charge on the efficiency of the separation of the photoproduced charge carriers. The photoionization efficiencies of 8-HOP and 8-MOP are similar. It is concluded that the intersystem crossing properties of BrMOP show a substantial heavy atom effect, whereas the effects of hydrophobic substitution on photoionization efficiency are minor.
Applied Catalysis B-environmental | 2006
Josef Krýsa; Georg Waldner; Hana Měšt’ánková; Jaromír Jirkovský; Gottfried Grabner
Chemistry: A European Journal | 2008
Ilse Manet; Sandra Monti; Gottfried Grabner; Stefano Protti; Daniele Dondi; Valentina Dichiarante; Maurizio Fagnoni; Angelo Albini
Macromolecules | 2012
Markus Griesser; Claudia Dworak; Sigrid Jauk; Michael Hofer; Arnulf Rosspeintner; Gottfried Grabner; Robert Liska; Georg Gescheidt
Journal of Photochemistry and Photobiology A-chemistry | 2006
Bernhard Seidl; Robert Liska; Gottfried Grabner
ChemPhysChem | 2007
Krisztina Pál; Mihály Kállay; Gottfried Köhler; Hong Zhang; István Bitter; Miklós Kubinyi; Tamás Vidóczy; Gottfried Grabner
Physical Chemistry Chemical Physics | 2010
Claire Richard; Paul Krajnik; Gottfried Grabner
Archive | 2006
Helena Dodziuk; Witold Danikiewicz; Gottfried Grabner; Daniel Krois; Udo H. Brinker; Renata Bilewicz; Kazimierz Chmurski; Masashi Kunitake; Akihiro Ohira