Graciela A. Oliva
National University of La Plata
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Publication
Featured researches published by Graciela A. Oliva.
Brazilian Journal of Medical and Biological Research | 1998
Cecilia Mónica Galosi; Junzo Norimine; María Gabriela Echeverría; Graciela A. Oliva; Edgardo Omar Nosetto; María Elisa Etcheverrigaray; Y. Tohya; Takeshi Mikami
The genomes of 10 equine herpesvirus 1 (EHV-1) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, BamHI and BglII, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype.
Pesquisa Veterinaria Brasileira | 1999
Ester Teresa González; Junzo Norimine; Alejandro Rafael Valera; Gabriel Eduardo Travería; Graciela A. Oliva; María Elisa Etcheverrigaray
Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). In Argentina, where a program to eradicate EBL has been introduced, sensitive and reliable diagnosis has attained high priority. Although the importance of the agar gel immunodiffusion test remains unchanged for routine work, an additional diagnostic technique is necessary to confirm cases of sera with equivocal results or of calves carrying maternal antibodies.Utilizing a nested shuttle polymerase chain reaction, the proviral DNA was detected from cows experimentally infected with as little as 5 ml of whole blood from BLV seropositive cows that were nonetheless normal in haematological terms. It proved to be a very sensitive technique, since it rapidly revealed the presence of the provirus, frequently at 2 weeks postinoculation and using a two-round procedure of nested PCR taking only 3 hours. Additionally, the primers used flanked a portion of the viral genome often employed to differentiate BLV type applying BamHI digestion. It is concluded that this method might offer a highly promising diagnostic tool for BLV infection.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2004
Eduardo Mortola; Graciela A. Oliva; Miguel Atilio Risso; Marcelo Ricardo Pecoraro; M.C. Venturini
This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 1999
E.T. Gonzalez; Graciela A. Oliva; J. Norimine; V. Cid de la Paz; María Gabriela Echeverría
A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the antibodies directed to p24 appeared early (between the 2nd and 4th week post inoculation) and thereafter antibodies to gp51were detected in some animals. The analysis of field serum samples by AGID as compared to WB showed an agreement of 90.9%. Only 1.7% of sera were negative by AGID and positive by WB and 7.2% that were not conclusive by AGID and were defined by WB (4.2% as positive and 3.0% as negative).
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health | 2001
Cecilia Mónica Galosi; M. V. Vila Roza; Graciela A. Oliva; M. R. Pecoraro; M. G. Echeverría; S.G. Corva; María Elisa Etcheverrigaray
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health | 2010
Edgardo Omar Nosetto; María Elisa Etcheverrigaray; Graciela A. Oliva; Ester Teresa González; S. A. Samus
Journal of Veterinary Medical Science | 1994
María Gabriela Echeverría; Junzo Norimine; Cecilia Mónica Galosi; Graciela A. Oliva; María Elisa Etcheverrigaray; Edgardo Omar Nosetto; Yukinobu Tohya; Takeshi Mikami
Analecta Veterinaria | 1998
Cecilia Mónica Galosi; María Gabriela Echeverría; M. V. Vila Roza; Viviana Cid de La Paz; Graciela A. Oliva; María Elisa Etcheverrigaray
Analecta Veterinaria | 2001
Ester Teresa González; Graciela A. Oliva; Alejandro Rafael Valera; Estela Bonzo; María Licursi; María Elisa Etcheverrigaray
Revista Argentina De Microbiologia | 2000
M. V. Vila Roza; Cecilia Mónica Galosi; Graciela A. Oliva; María Gabriela Echeverría; Marcelo Ricardo Ítalo Pecoraro; S.G. Corva; María Elisa Etcheverrigaray