Grant Kirker

Laboratory Evaluations of Durability of Southern Pine Pressure Treated With Extractives From Durable Wood Species.

Abstract Extracts from sawdust of four naturally durable wood species [Alaskan yellow cedar, AYC, Cupressus nootkanansis D. Don 1824; eastern red cedar, ERC, Juniperus virginiana L.; honey mesquite, HM, Prosopis glandulosa Torr.; and black locust, BL, Robinia pseudoacacia L.] were used to treat southern pine, Pt, Pinus taeda L. sapwood blocks. Extractive treated blocks were evaluated for decay resistance in standard soil bottle fungal assays challenged with brown and white rot decay fungi. Results showed that extractives did impart some improvement to decay resistance of Pt blocks. BL- and HM-treated Pt blocks were also used in choice and no-choice assays to determine feeding preference and damage by eastern subterranean termites (Reticulitermes flavipes) Kollar. Minimal feeding on treated blocks was seen in both choice and no-choice assays. In choice assays, there was similar mortality between HM and BL arenas; however, in no-choice assays, complete mortality was recorded for HM-treated Pt and high mortality was seen with BL-treated Pt. Subsequent dose mortality termite assays showed HM to be effective in killing R. flavipes at low concentrations. Both HM and BL show promise as deterrents or termiticidal protectants and will be further evaluated in field studies.

Long-Term Durability of Pressure-Treated Wood in a Severe Test Site

Improved estimates of the long-term durability of treated wood products are needed to guide choices about construction materials and allow estimates of design life. This report summarizes the long-term decay and insect resistance of treated wood post and lumber specimens placed in ground contact at a test site of the U.S. Department of Agriculture, Forest Service, Forest Product Laboratory in southern Mississippi. Posts treated with relatively low levels of creosote had an estimated durability of 54 years, and slightly less durability was observed in creosote-treated lumber. Pentachlorophenol-treated posts exhibited durability in excess of 60 years, whereas lumber specimens treated to standard ground-contact retentions had no failures after 39 or 45 years. Posts treated with low retentions of copper naphthenate had an estimated 65-year longevity, but lumber specimens treated to higher retentions of copper napththenate had lower average lives of 27 to 30 years. Low-retention ammoniacal copper arsenate (ACA) posts had an estimated durability of 60 years, whereas stakes treated to retentions of 8 kg/m3 (0.5 lb/ft3) or greater with ammoniacal copper zinc arsenate (ACZA) or ACA have had no failures after 30 and 60 years, respectively. Posts treated with a range of retentions of chromated copper arsenate (CCA-C) have had no failures after 35 years, and stakes treated with CCA-A, CCA-B, or CCA-C to retentions above 7 0 kg/m3 (0.43 lb/ft3) have had no failures after 60, 61, and 40 years, respectively. As a whole, the post and lumber specimens indicate an expected durability of over 50 years for creosote-treated wood and over 60 years for wood treated with pentachlorophenol, copper naphthenate, ACZA, or CCA. Comparison of the results from this site to reports from other locations suggests that these results might underestimate the potential durability in more moderate exposures. In relating these findings to treated commodities, it should be noted that these test specimens have not been subjected to the same mechanical loads or wear and tear associated with in-service structures.

Fungal–copper interactions in wood examined with large field of view synchrotron-based X-ray fluorescence microscopy

ABSTRACT The goal of this study was to demonstrate how synchrotron-based X-ray fluorescence microscopy (XFM) can be used to better understand the mechanisms of copper tolerance in wood decay fungi. Copper is a major component in commercial wood preservatives as it is toxic to many wood decay fungi. However, certain fungi are copper tolerant and can attack preservative-treated wood, resulting in structural damage to treated wood members. Here we used large-field XFM to visualize six different elements (K, Ca, Mn, Fe, Cu, and Zn) in the mycelia and wood inoculated with four different species of brown rot wood decay fungi. Wood blocks were partially dipped into a solution of copper sulfate, exposed to fungi in malt extract agar petri dish assays for nine weeks, and then imaged and compared to blocks that were partially dipped in water. The blocks were imaged immediately adjacent to an end-matched control that was placed in malt extract agar petri dish assays for 9 weeks, but not exposed to the fungi so that the differences in the elemental distributions could be directly compared. The colonized wood and mycelia were rich in K, Ca, Mn, and Fe; however, the elements and the spatial distribution in the mycelia and wood differed across fungal species. The most interesting results were the maps showing the copper distribution. While three of the four fungi grew on the copper-rich region of the wood, only one species, Fibroporia radiculosa, dramatically reduced the copper concentration in the region of fungal growth.

Antioxidant Effects of Four Heartwood Extractives on Midgut Enzyme Activity in Heterotermes indicola (Blattodea: Rhinotermitidae)

Abstract Heterotermes indicola (Wasmann) (Blattodea: Rhinotermitidae) is a species of subterranean termite that is a destructive pest of wood and wood products in Pakistan. This study evaluated the antioxidant and antienzyme potential of heartwood extractives against H. indicola. Heartwood extractives of four durable wood species, Tectona grandis (L.f), Dalbergia sissoo (Roxb.), Cedrus deodara (Roxb.), and Pinus roxburghii (Sarg.) were removed from wood shavings via soxhlet extraction with an ethanol:toluene solvent system. The antioxidant potential of the extractive compounds was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging test. Results showed maximum antioxidant activity for extractives of D. sissoo. D. sissoo had the lowest IC50 (the concentration where 50% inhibition of the DPPH radical is obtained) at 28.83 μg/ml among the heartwood extractives evaluated. This antioxidant activity, however, was not concentration dependent as was observed in the other heartwood extractives tested. At the maximum test concentration, T. grandis showed the highest percent inhibition at 89.7%, but this inhibition was lower compared to the positive control antioxidant compounds butylated hydroxytoluene and quercetin. When termites were fed filter paper treated with IC50s of the extractives and control compounds, glutathione S-transferase activity in the guts of H. indicola workers was significantly reduced by T. grandis and D. sissoo extractives. Similarly, esterase activity was reduced more by P. roxburghii extractives compared to control antioxidant treatments and other tested extractives. However, none of the extractives examined significantly reduced the activity of catalase enzymes in H. indicola compared to treatments with the antioxidant control compounds.

Amplicon-Based Sequencing of Soil Fungi from Wood Preservative Test Sites

Soil samples were collected from field sites in two AWPA (American Wood Protection Association) wood decay hazard zones in North America. Two field plots at each site were exposed to differing preservative chemistries via in-ground installations of treated wood stakes for approximately 50 years. The purpose of this study is to characterize soil fungal species and to determine if long term exposure to various wood preservatives impacts soil fungal community composition. Soil fungal communities were compared using amplicon-based DNA sequencing of the internal transcribed spacer 1 (ITS1) region of the rDNA array. Data show that soil fungal community composition differs significantly between the two sites and that long-term exposure to different preservative chemistries is correlated with different species composition of soil fungi. However, chemical analyses using ICP-OES found levels of select residual preservative actives (copper, chromium and arsenic) to be similar to naturally occurring levels in unexposed areas. A list of indicator species was compiled for each treatment-site combination; functional guild analyses indicate that long-term exposure to wood preservatives may have both detrimental and stimulatory effects on soil fungal species composition. Fungi with demonstrated capacity to degrade industrial pollutants were found to be highly correlated with areas that experienced long-term exposure to preservative testing.

Fifty-Year Durability Evaluation of Posts Treated with Industrial Wood Preservatives

Abstract Long-term durability data are needed to improve service life estimates for treated wood products used as critical structural supports in industrial applications. This article reports the durability of longleaf pine (Pinus palustris) posts pressure treated with ammoniacal copper arsenate (ACA), chromated copper arsenate (CCA), creosote, or pentachlorophenol and exposed for 50 years in southern Mississippi. During inspections, posts were subjected to a pass/fail evaluation by applying a load to the top of the post. No failures occurred in any of the 125 posts treated with CCA or in any of the 75 posts treated with pentachlorophenol. Three of 25 ACA-treated posts and 5 of 25 creosote-treated posts failed. Estimated times to 50 percent failure in the ACA- and creosote-treated posts were calculated as 96 and 78 years, respectively. The estimated years to failure for the CCA- and pentachlorophenol-treated posts could not be calculated because of the lack of failures but presumably would be greater than...

Effects of White Mulberry (Morus alba) Heartwood Extract Against Reticulitermes flavipes (Blattodea: Rhinotermitidae)

Abstract Heartwood extract from white mulberry (Morus alba L.) (Rosales: Moraceae) were investigated for antitermitic activity against Reticulitermes flavipes (Kollar) (Blattodea: Rhinotermitidae) in laboratory experiments. An ethanol:toluene (2:1) solvent system was used to remove extract from heartwood shavings. A concentration-dependent feeding response and mortality were observed for termites exposed to a concentration series range of 1.25 to 10 mg/ml of extract based on their dry weight. Results showed that maximum termite mortality occurred at 10 mg/ml. Based on the concentration series data, LC50 was calculated at 1.71 mg/ml. In filter paper feeding and repellency assays, extract significantly decreased the total number of gut protozoa compared with untreated and solvent controls. After feeding on filter paper treated at 10 mg/ml for 2 wk, protozoan populations were reduced by >55%. In choice and no-choice tests with mulberry heartwood, greater wood loss from termite feeding was found on solvent extracted blocks compared with nonextracted. Complete (100%) mortality was observed after feeding on nonextracted blocks compared with extracted blocks. Heartwood extract from white mulberry imparted resistance to vacuum pressure treated, nondurable southern pine and cottonwood. At every concentration tested, 100% mortality was observed after feeding on extract-treated southern pine or cottonwood. GC-MS analysis of extract showed high levels of the phenol compound, resorcinol. Results indicated that heartwood extract from white mulberry have antitermitic properties and might be potentially valuable in the development of environmentally benign termiticides.

Dual Protection of Wood Surface Treated with Melamine-Modified Urea-Formaldehyde Resin Mixed with Ammonium Polyphosphate against Both Fire and Decay

Abstract Surface coatings of melamine-modified urea-formaldehyde resins (MUFs) containing ammonium polyphosphate (APP) have been shown to significantly improve the fire retardancy of wood by prolon...