Grant Wayne Tempero

Age composition, growth, and reproduction of koi carp (Cyprinus carpio) in the lower Waikato region, New Zealand

Abstract A total of 566 koi carp (Cyprinus carpio) from the lower Waikato region were aged from scales and opercular bones, and growth was modelled with the von Bertalanffy growth function. There was no difference in growth rate between male and female carp. Growth of koi carp between zero and 3 years of age was lower than that of common carp in Europe and Australia. However, after 5 years of age the growth of koi carp was higher than that of common carp in Europe, but still below that of carp in Australia. Males rarely lived in excess of 8 years, whereas females lived to 12 years. Mean total fecundity calculated from 44 running‐ripe females was 299000 oocytes (±195600 SD) (range 29800–771000). Relative fecundity ranged from 19300 to 216000 oocytes kg‐1 total body weight, with a mean of 97 200 (±35 000 SD) oocytes kg‐1. Feral koi carp in the Waikato are capable of multiple spawnings within their lifetimes. Within a spawning season, Waikato populations of feral koi carp contained females that spawned once, and females that had the potential to have spawned repeatedly. Female gonadosomatic index (GSI) varied with season and was negatively related to water temperature.

A field experiment on selective baiting and bait preferences of pest mustelids (Mustela spp.)

Abstract We tested whether a new automated bait dispenser, the Scentinel®, could be programmed to deliver non-toxic bait selectively only to animals of specified dimensions (>400 g body weight and <110 mm body diameter, the size of a ferret or a mink). We tested whether wild ferrets would discriminate between two types of bait (egg/oil and homogenised brain tissue). We set 24 Scentinels in 12 randomised pairs for 11 weeks on a 2400-ha area of pastoral farmland in New Zealand. Of 1559 visits by small animals, 198 were by ferrets (c. 28 individuals). The Scentinels correctly delivered 895 baits to visitors weighing >400 g, and no baits to 543 visitors of <400 g. The mean body weight of live ferrets recorded by Scentinels was not significantly different from that of 34 carcasses collected from the same area by professional trappers. Of the 145 baits dispensed to ferrets, egg/oil bait was significantly more often eaten. We conclude that the Scentinel can reliably deliver bait to specified sizes of animals and deny it to others. It can also facilitate extensive bait preference tests on unrestrained wild pests.

Comparison of injuries to New Zealand rock lobsters (Jasus edwardsii) caused by hand versus snare collection

Abstract Following the prohibition in New Zealand of lobster snares in late 2005, we undertook research to compare the frequency and extent of injuries to rock lobsters (Jasus edwardsii) (Hutton) caused by recreational SCUBA divers using lobster snares compared with hand collection. Rock lobsters were sampled between January 2006 and October 2006 from multiple dive sites around the North Island of New Zealand. Of the 124 rock lobsters caught, 20.9% were in a soft shell state. Female rock lobsters constituted 43.5% of the catch, but only one was carrying eggs. Female mean tail width was 72.2 mm; male mean tail width was 71.9 mm. We found that hand collection caused significantly more injuries than snare collection, to both soft shell and hard shell animals. Hand collection also resulted in more major injuries, with 18% of hard shell animals and 31% of soft shell animals losing two or more limbs. We recommend that the prohibition on the use of rock lobster snares be lifted, as their use appears to significantly reduce injury and stress to rock lobsters in recreational dive areas, and increase the survival of undersized individuals released after capture.

The effect of GABA, salt and hydrocortisone on possum (Trichosurus vulpecula) ion homeostasis - a potential marsupial-specific toxin?

Abstract It has been observed that the γ-amino butyric acid (GABA) receptor in possums reacts differently from that of placental mammals, in that GABA coupled with high salt intake leads to severe calcium imbalance and death. Thus, the potential of a marsupial-specific toxin (MST) was trialled on wild, captive possums. Seven groups of possums (n=13–15) were tested with mixtures containing 20% GABA and 2, 5 or 8% salt, with or without a chemical stressor, hydrocortisone, plus an untreated control group. Blood analyte data showed that plasma glucose concentration, measured by glucose oxidase method, increased in the control and hydrocortisone-treated possums. Ion homeostasis (potassium and sodium chloride, measured by ion-selective electrodes, and phosphate measured by phosphorus-colourimetric assay) was moderately affected in the MST-treated groups at higher salt concentrations. Calcium was affected in all but two of the treatment groups (2% and 8% plus hydrocortisone). Despite this disruption of ion homeostasis, there was no mortality due to the effect of the MST. This was possibly due to an aversion to the feed. Thus the development of an MST was unsuccessful.

Combating hypoxia/anoxia at sediment-water interfaces: A preliminary study of oxygen nanobubble modified clay materials

Combating hypoxia/anoxia is an increasingly common need for restoring natural waters suffering from eutrophication. Oxygen nanobubble modified natural particles were investigated for mitigating hypoxia/anoxia at the sediment-water interface (SWI) in a simulated column experiment. By adding oxygen nanobubble modified zeolites (ONMZ) and local soils (ONMS), the oxygen nanobubble concentrations (105-107 particles/mL) were several orders of magnitude higher in the water than the original water solution (104 particles/mL) within 24 h. In the column experiment, an oxygen-locking surface sediment layer was formed after capping with ONMZ and ONMS particles. The synergy of diffusion of oxygen nanobubbles and retention of oxygen in this layer contributes to both the increase of DO and reversal of hypoxic conditions. The overlying water had significantly higher dissolved oxygen (DO) values (4-7.5 mg/L) over the experimental period of 127 days in ONMZ and ONMS compared with the control systems (around 1 mg/L). Moreover, the oxidation-reduction potential (ORP) was reversed from -200 mV to 180-210 mV and maintained positive values for 89 days in ONMZ systems. In the control systems, ORP was consistently negative and decreased from -200 mV to -350 mV. The total phosphorus (TP) flux from sediment to water across the SWI was negative in the ONMZ and ONMS treated systems, but positive in the control system, indicating the sediment could be switched from TP source to sink. The oxygen-locking capping layer was crucial in preventing oxygen consumption caused by the reduced substances released from the anoxic sediment. The study outlines a potentially promising technology for mitigating sediment anoxia and controlling nutrient release from sediments, which could contribute significantly to addressing eutrophication and ecological restoration.