Grazia Lombardo

Saffron, An Alternative Crop for Sustainable Agricultural Systems: A Review

Saffron (Crocus sativus L.) is an autumnal flowering geophite whose dried stigmas, well known for their aromatic and colouring power, have been used since immemorial time as a spice in human nutrition, for medicinal purposes and as a dye. Many doubts remain on its origin; it was probably selected and domesticated in Crete during the Late Bronze Age. Saffron is an autotriploid geophyte species, self- and out-sterile and mostly male-sterile and therefore unable to produce seed, that reproduces by means of corms. Furthermore, it has a reverse biological cycle compared with the majority of cultivated and spontaneous plants: flowering first in October–November, then vegetative development until May, which means that the vegetative development is not directly important for production of stigmas, but for the production of new corms. Due to its unique biological, physiological and agronomic traits, saffron is able to exploit marginal land and to be included in low-input cropping systems, representing an alternative viable crop for sustainable agriculture. Notwithstanding this great potential and the considerable increase in new generation consumer demand for saffron, the future of the plant is still uncertain. Indeed, the main obstacles to saffron production are: (1) the limited areas of cultivation in countries where it is traditionally grown, (2) the great amount of sophisticated spice, (3) management techniques executed by hand, and (4) the very high price of the spice. Here we review the main biological, genetic and ecological traits associated with agronomic management techniques of saffron in relation to environmental conditions. Colour, taste and aroma are the essential features on which the quality of saffron stigmas is founded. In turn, these aspects are strictly connected with the biomolecular composition of the stigmas, namely, the carotenoids and their derivatives. With this in mind, the biosynthetic pathway that leads to the formation of saffron secondary metabolites and their abundance in the spice is presented, together with the biomedical properties commonly associated with saffron. Furthermore, a detailed overview of the more recent instrumental methods to assess the quality of saffron, strictly from a chemical point of view, will be discussed.

Agronomic, chemical and genetic variability of saffron (Crocus sativus L.) of different origin by LC-UV–vis-DAD and AFLP analyses

The identification of a bi-univocal correspondence between geographical origin of saffron (Crocus sativus L.) and the composition of its stigmas has recently been the subject of many research papers, which have focused on the analysis of the differences among the so called “minor components”, such as flavonoids and volatiles, in the secondary metabolic pattern of this spice. Saffron pigments (crocetin esters), on the other hand, constitute the majority of the metabolites found in its stigmas, and their spectrophotometric measurement is still used as an official method to determine the quality of the spice in terms of coloring power. To our knowledge, no attempts have been made to find a correspondence between the geographical origin of different saffron samples and their morphological traits and pigments pattern. In this paper, we have demonstrated that saffron corms of different origins, grown in the same experimental field, produce daughter corms with different dimensions and still produce stigma samples with different pigment profiles. Furthermore, daughter corm dimensions and pigment profile even more so, may be related to the origin of the sample, and therefore pigments can be used as chemotaxonomic markers. Compositional analyses results were corroborated by genetic data obtained using AFLP molecular markers.

Agronomic Characterization and α- and β‑ODAP Determination through the Adoption of New Analytical Strategies (HPLC-ELSD and NMR) of Ten Sicilian Accessions of Grass Pea

Ten accessions of grass pea (Lathyrus sativus L.) from different Sicilian sites, cultivated in the same environmental conditions, were analyzed for their morphological and productive parameters and for the content of two non-protein amino acids: α- and β-ODAP (α- and β-N-oxalyl-l-α,β-diaminopropionic acid). The β-isomer is the neurotoxin responsible for the neuron disease known as lathyrism. This analysis was carried out using two common analytical methodologies never applied in their determination, an HPLC separation with evaporative light scattering (ELS) as detector, and nuclear magnetic resonance (NMR). The content of the two isomers falls in the range reported for these compounds: 0.42-0.74 and 2.69-4.59 g/kg for α- and β-ODAP, respectively; and the two methods yield comparable results. High productivity and a high protein content were detected in three Sicilian accessions. Low β-ODAP content was found to be linked to accessions with heavier seeds and those originating at lower altitudes.

Rehabilitation strategies for energy-efficient buildings in GeoCluster

The concept of GeoCluster is a virtual homogeneous trans-regional area where strong similarities are found in terms of climate, environmental context, culture, construction typologies. It allows to export relevant results and guidelines, studied in a specific area, in others trans-regional areas with strong similarities. Present research regards the modern building heritage constituting framed buildings that represent, at the international level, a large part of the existing heritage and is characterized by poor performance in environmental and energetic aspects. In particular, this paper identifies guidelines to approach the optimization of the intervention for the energetic restoration of a buildings heritage belonging to the GeoCluster that we call “Mediterranean”.

Volatile profiling of durum wheat kernels by HS–SPME/GC–MS

This work aimed at developing a HS–SPME/GC–MS method for the extraction and analysis of volatile compounds from the kernels of durum wheat. Fiber coating, temperature and time of extraction were evaluated. Moreover, the method was used as a tool for varietal characterization. The qualitative and semi-quantitative characterization of the volatile fraction of durum wheat kernels highlighted 11 different chemical classes, alcohols and aldehydes prevailing over acids, alkanes, aromatic and heterocyclic hydrocarbons. Concerning the comparison among the studied durum wheat kernel cultivars, Sculptur was the one with the highest amount and variability of volatile compounds. This cultivar stood out for the highest level of alkanes and ketones, while Anco Marzio contained higher levels of aldehydes and alkenes. The optimized method allowed for the qualitative and semi-quantitative characterization of the volatile fraction of durum wheat kernels, and it was effective at differentiating durum wheat cultivars based on their different volatile profiles, representing a potential tool for varietal selection and for the exploitation of specific durum wheat cultivars.

Simulation analysis of improved envelope measures for modern buildings in the Mediterranean climate

In Europe, research has the objective to produce guidelines for building refurbishment of modern heritage. In particular, the issue of energetic improvement is analysed according to characteristics of existing heritage and local climate. In fact, it is possible to observe that, locally, building production after World War II is composed of common building types built with recurrent constructive techniques and technological solutions. Based on this, a research has been initiated with the objective to identify procedures and the sequence of actions to be applied for rehabilitation of modern heritage buildings throughout the Italian territory. This paper reports first results of the assessment of the energy requirement for cooling and heating, for different interventions on the envelope, for a very common building type in the existing modern heritage buildings in Eastern Sicily.

Traditional Building Materials for Innovative Envelope

The present paper is part of a research that is developed within the sustainable building design through the revisiting of the traditional construction materials. The results obtained show that the natural stone, enhanced by technological innovations, are often capable of providing excellent performance. Based on the tests, it was possible to verify and validate the hypothesis that the proposed new system of external vertical opaque enclosure consisting in a panel in dry-assembled and pre-compressed blocks of natural stone through reinforcing steel, has good performances when used both in the case of new design in the case of recovery of modern buildings, when the intervention is being addressed within of an overall building improvement regarding the security, sustainability, functionality and image. This paper reports the first results obtained by the study of the feasibility of the envelope being tested, through the definition of all the details of links with the existing building structure.