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Biological Trace Element Research | 1994

The influence of color, age, and sex on the content of zinc, copper, nickel, manganese, and lead in human hair

Alberto Sturaro; Giorgio Parvoli; Lucio Doretti; Graziella Allegri; Carlo Virgilio Luigi Costa

The hair of 132 healthy subjects between 6 and 40 yr old living in the Veneto region in Italy was analyzed by means of HPLC method in order to determine the presence of zinc, copper, nickel, manganese, and lead. The collected samples were subdivided on the basis of age (6–11 and 19–40 yr), and sex and color (black, red, brown, and blond). From the data some evident differences were emphasized. In female hair the content of metals was higher than in male hair independently of color. Blond hair gave the lowest concentration values of the elements studied independently of sex. The maximum amount of the metals was found generally in black hair, followed by red and brown hair. Age seems to have a different influence, with the copper element decreasing appreciably in brown and blond female hair as the age of the subjects increased.


Clinica Chimica Acta | 1963

The metabolism of tryptophan in patients with bladder cancer and other urological diseases

C. A. Benassi; B. Perissinotto; Graziella Allegri

Abstract The excretion of tryptophan metabolites was followed in the urine of 201 patients with bladder tumour, of 55 subjects with cancer of genito-urinary organs (ex. tra-bladder), and of 112 patients with various urological diseases (non-neoplastic). All 3 groups contained patients excreting abnormal amounts of kynurenine, 3-hydroxykynurenine and 3-hydroxyanthranilic acid. The urinary kynurenine excretion was followed for several days in 9 bladder tumour patients during simultaneous administration of daily large doses of vitamin B 6 . In some cases pyridoxine slightly decreased, but never completely inhibited the abnormal excretion of kynurenine. In addition to these investigations on spontaneous excretion, l -tryptophan was administered (100 mg/kg body weight) to 4 bladder tumour patients. Kynurenine, N -α-acetylkynurenine, 3-hydroxykynurenine, kynurenic acid, xanthurenic acid and 3-hydroxyanthranilic acid were measured after loading. Small differences in the sum of compounds excreted were found between these patients and normal controls. The data presented are difficult to reconcile with the hypothesis that the excretion of abnormal quantities of tryptophan metabolites, suggested as endogenous carcinogens, might have caused the induction of cancer only when excreted by bladder tumour patients. In fact such an excretion occurs in patients with extra-bladder tumours and with various urological diseases, as well as in other human pathological conditions.


Rapid Communications in Mass Spectrometry | 2000

Protein profiles in sera of patients with malignant cutaneous melanoma

Lorenza Ferrari; Roberta Seraglia; Carlo Riccardo Rossi; Antonella Bertazzo; Mario Lise; Graziella Allegri; Pietro Traldi

Seventeen samples of sera from patients with malignant cutaneous melanoma at various stages and 14 samples from healthy subjects were analysed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Results highlighted the presence of several protein species at molecular weights lower than 30000 Da, presumably originating from proteolysis, in the sera of the patients with melanoma. These species were completely absent in healthy subjects. In particular, the presence and abundance of species with molecular weights in the range 2500-3500 Da exhibit significant variations related to the different clinical stages of the disease.


Biological Trace Element Research | 1996

Determination of copper and zinc levels in human hair: influence of sex, age, and hair pigmentation.

Antonella Bertazzo; Carlo Virgilio Luigi Costa; M. Biasiolo; Graziella Allegri; Girolamo Cirrincione; Giuseppe Presti

The Cu and Zn levels of both 607 men (1–85 y old) and 649 women (1–92 y old) were determined by atomic absorption spectrometry. Sex does not influence Cu (14.89±0.89 μg/g and 15.26±0.79 μg/g hair for males and females, respectively) and Zn contents (200.97±9.68 μg/g for men and 209.81±9.49 μg/g hair for women). Age influences Cu and Zn concentrations, but only significantly in females: Cu levels decrease over 60 y of age; whereas Zn levels increase significantly from age groups 2–5 to 20–40 years. Hair color influences Cu concentrations in both males and females. In males, white hair containes less Cu than black hair; in females, white hairs Cu levels are significantly lower than those of dark blond, red, light brown, and brown hair. There are no significant differences in Zn concentrations with respect to different hair colors, in either males or females.


Biochimica et Biophysica Acta | 2001

Enzyme activities involved in tryptophan metabolism along the kynurenine pathway in rabbits

Antonella Bertazzo; Eugenio Ragazzi; M. Biasiolo; Carlo Virgilio Luigi Costa; Graziella Allegri

The following enzyme activities of the tryptophan-nicotinic acid pathway were studied in male New Zealand rabbits: liver tryptophan 2,3-dioxygenase, intestine indole 2,3-dioxygenase, liver and kidney kynurenine 3-monooxygenase, kynureninase, kynurenine-oxoglutarate transaminase, 3-hydroxyanthranilate 3,4-dioxygenase, and aminocarboxymuconate-semialdehyde decarboxylase. Intestine superoxide dismutase and serum tryptophan were also determined. Liver tryptophan 2,3-dioxygenase exists only as holoenzyme, but intestine indole 2,3-dioxygenase is very active and can be considered the key enzyme which determines how much tryptophan enters the kynurenine pathway also under physiological conditions. The elevated activity of indole 2,3-dioxygenase in the rabbit intestine could be related to the low activity of superoxide dismutase found in intestine. Kynurenine 3-monooxygenase appeared more active than kynurenine-oxoglutarate transaminase and kynureninase, suggesting that perhaps a major portion of kynurenine available from tryptophan may be metabolized to give 3-hydroxyanthranilic acid, the precursor of nicotinic acid. In fact, 3-hydroxyanthranilate 3,4-dioxygenase is much more active than the other previous enzymes of the kynurenine pathway. In the rabbit liver 3-hydroxyanthranilate 3,4-dioxygenase and aminocarboxymuconate-semialdehyde decarboxylase show similar activities, but in the kidney 3-hydroxyanthranilate 3,4-dioxygenase activity is almost double. These data suggest that in rabbit tryptophan is mainly metabolized along the kynurenine pathway. Therefore, the rabbit can also be a suitable model for studying tryptophan metabolism in pathological conditions.


Advances in Experimental Medicine and Biology | 1999

Tryptophan Catabolism in Synovial Fluid of Various Arthropathies and its Relationship with Inflammatory Cytokines

Antonella Bertazzo; Leonardo Punzi; Nidia Bertazzolo; M. Pianon; Assunta Pozzuoli; Carlo Virgilio Luigi Costa; Graziella Allegri

Synovial fluids (SF) from patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), gout, and osteoarthritis (OA) were investigated for the levels of interleukin (IL)-1 beta, IL-6 and IL-8, tryptophan (Trp) and indoleamine 2,3-dioxygenase (IDO) activity. Significant differences exist in the levels of IL-1 beta between inflammatory arthritides RA, PsA and gout and non inflammatory arthritis, such as OA. The highest concentration of IL-1 beta was found in RA, that showed high levels also of IL-6 and IL-8. In the same disease we also found the highest IDO activity and the lowest Trp concentration. In addition, IDO activity seems to be related with the decrease in Trp, as demonstrated by the inverse correlation found between these two substances in the SF of all patients.


Farmaco | 2003

Enzyme activities of tryptophan metabolism along the kynurenine pathway in various species of animals.

Graziella Allegri; Carlo Virgilio Luigi Costa; Antonella Bertazzo; M. Biasiolo; Eugenio Ragazzi

The purpose of this study was to investigate variations in the enzyme activities of the kynurenine pathway in various mammals (rabbit, mouse, rat, guinea-pig). Liver tryptophan 2,3-dioxygenase, small intestine indole 2,3-dioxygenase, liver and kidney kynurenine 3-monooxygenase, kynureninase, kynurenine-oxoglutarate transaminase, 3-hydroxyanthranilate 3,4-dioxygenase and aminocarboxymuconate-semialdehyde decarboxylase were analysed. Small intestine superoxide dismutase activity and free and total serum tryptophan were also measured. Liver tryptophan 2,3-dioxygenase was present as both holoenzyme and apoenzyme only in rat, while in the other species only holoenzyme activity was observed. Also, small intestine indole 2,3-dioxygenase activity was more abundant in rat than in the other animals studied. The highest activity of small intestine superoxide dismutase was found in rat, and the lowest in rabbit. Liver and kidney kynurenine 3-monooxygenase activity was very elevated and higher in mouse, followed by rat; rabbit showed the lowest activity. Kynureninase activity appeared to be much lower among the enzymes of the kynurenine pathway. However, guinea-pig showed higher activity in both liver and kidney in comparison with other species. With regard to kynurenine-oxoglutarate transaminase, all species examined here presented more abundant enzyme activity in kidney, the value being similar between rat and mouse. Guinea-pig was the animal with the lowest activity. 3-Hydroxyanthranilate 3,4-dioxygenase showed the highest activity of all the enzymes evaluated in the study, but with different levels in liver and kidney, varying among species. The most elevated activity of aminocarboxymuconate-semialdehyde decarboxylase was present in kidney of guinea-pig, and the lowest in rabbit. Serum concentrations of tryptophan were higher in rat, followed by mouse, rabbit and guinea-pig. In conclusion, the present study demonstrates that the enzyme activities of the kynurenine pathway are very active in tissues of the four species of mammals investigated. The proposed method of in vitro enzyme determination represents a valid alternative to study of the tryptophan metabolic route.


Advances in Experimental Medicine and Biology | 1999

Importance of L-Tryptophan Metabolism in Trypanosomiasis

P. Vincendeau; S. Lesthelle; Antonella Bertazzo; M. C. Okomoassoumou; Graziella Allegri; Carlo Virgilio Luigi Costa

African trypanosomiasis or sleeping sickness is caused by extracellular trypanosomes. The presence of seric antibodies directed to a tryptophan-like epitope in trypanosome infected patients and animals led us to investigate the roles of tryptophan in trypanosomiasis. These antibodies are directed against a tryptophan-rich conserved sequence inside the major parasite surface glycoprotein. In vitro, a rapid uptake of tryptophan by trypanosomes is measured. Seric tryptophan levels are decreased during trypanosomiasis. This decrease may be linked with an increase in indoleamine 2,3-dioxygenase (IDO) induced by Interferon-gamma. In vivo inhibition of IDO by norharman provokes a dramatic increase in circulating parasite number. All these data show the essential role of tryptophan in parasite growth. Moreover, antibodies against tryptophan, the decreased concentration of the neurotransmitter serotonin in the brain following infection and the tryptophan metabolites (tryptophol) produced by trypanosomes may participate to the pathophysiological mechanisms provoking sleeping sickness.


Advances in Experimental Medicine and Biology | 2003

Kynurenine pathway enzymes in different species of animals.

Graziella Allegri; Antonella Bertazzo; M. Biasiolo; Carlo Virgilio Luigi Costa; Eugenio Ragazzi

Kynurenine pathway enzyme activities, liver tryptophan 2,3-dioxygenase (TDO), small intestine indole 2,3-dioxygenase (IDO), liver and kidney kynurenine 3-monooxygenase, kynurenine-oxoglutarate transaminase, kynureninase, 3-hydroxyanthranilate 3,4-dioxygenase and aminocarboxymuconate-semialdehyde decarboxylase, were assayed in rabbits, rats, mice and guinea pigs. Their activities varied among species. Especially, TDO was present as both holoenzyme and apoenzyme only in rat, while the other species, rabbit, mouse and guinea pig, only showed holoenzyme activity. Mitochondrial liver and kidney kynurenine 3-monooxygenase activities were much higher in mouse and rat, with rabbit showing the lowest activity. Kynureninase activity showed similar values in both liver and kidney in each species. However, lower activity was present in rabbit. As regards kynurenine-oxoglutarate transaminase, the highest activity appeared in kidney, in all species studied. 3-Hydroxyanthranilate 3,4-dioxygenase activity showed different behaviour in the four species. In rabbit, its activity was higher in kidney than in liver; in rat and mouse, it was viceversa; and in guinea pig, both liver and kidney had similar activity. Instead, the activity of aminocarboxymuconate-semialdehyde decarboxylase was higher in kidney than in liver only in guinea pig. Serum tryptophan concentrations were also determined. Rabbit and guinea pig showed similar values, whereas in rat and mouse, serum tryptophan levels were higher, rat having the highest concentrations. In all species assayed, the free fraction was present as 11-12% of total tryptophan.


Biochimica et Biophysica Acta | 2002

Enzyme activities along the tryptophan-nicotinic acid pathway in alloxan diabetic rabbits

Eugenio Ragazzi; Carlo Virgilio Luigi Costa; Laura Caparrotta; M. Biasiolo; Antonella Bertazzo; Graziella Allegri

Recent data from our laboratory have indicated that the rabbit is a suitable animal model for the study of enzyme activities of the tryptophan-nicotinic acid pathway. We report here the pattern of tryptophan metabolism in rabbits made diabetic with alloxan treatment, and hypercholesterolemic with a high-cholesterol diet. A group of rabbits with only hypercholesterolemia was also considered. The enzymes assayed were: liver tryptophan 2,3-dioxygenase (TDO), intestine indoleamine 2,3-dioxygenase (IDO), liver and kidney kynurenine 3-monooxygenase, kynurenine-oxoglutarate transaminase, kynureninase, 3-hydroxyanthranilate 3,4-dioxygenase and aminocarboxymuconate-semialdehyde decarboxylase.TDO showed a reduction of specific activity in liver of diabetic-hyperlipidemic and hyperlipidemic rabbits compared to controls. Intestine IDO activities and liver and kidney kynurenine monooxygenase were unchanged with respect to controls.Kynurenine-oxoglutarate transaminase and kynureninase activities were reduced in the kidneys, but not in the liver, of diabetic-hyperlipidemic rabbits. The main finding was the reduction of 3-hydroxyanthranilate 3,4-dioxygenase activity (expressed as activity per g of fresh tissue) in the liver and kidneys of diabetic-hypercholesterolemic and hyperlipidemic rabbits compared to controls. Conversely, aminocarboxymuconate-semialdehyde decarboxylase activity was significantly higher in diabetic hypercholesterolemic rabbits in comparison with control and hypercholesterolemic rabbits. These data demonstrate that also in diabetic rabbits there is an alteration of tryptophan metabolism at the level of 3-hydroxyanthranilic acid-->nicotinic acid step. Also dyslipidemia seems to be involved in enzyme activity variations of the tryptophan metabolism along the kynurenine pathway.

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Pietro Traldi

National Research Council

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