Greg T. Browne

The avocado subgroup of Phytophthora citricola constitutes a distinct species, Phytophthora mengei sp. nov.

Isolates from avocado tree cankers have been recognized as a distinct subgroup within the P. citricola complex since 1974, both morphologically and molecularly (isozyme and amplified fragment length polymorphism [AFLP] analyses). This subgroup is formally separated from P. citricola after comparative DNA fingerprinting and sequence analyses of the ITS region, as well as by morphological examinations. This new taxon is homothallic, produces plerotic oospores with paragynous antheridia and noncaducous semipapillate sporangia. Morphologically it differs from other species of Waterhouse group III by producing many large bizarre-shaped sporangia and smaller oogonia with asymmetric capitate antheridia. It belongs to clade 2 and is phylogenetically closer to P. siskiyouensis, P. capsici and P. tropicalis than to P. citricola. P. mengei can be easily differentiated from its relatives in the same clade and other species of this morpho-group by DNA fingerprints and sequence analysis. This new taxon is named Phytophthora mengei sp. nov.

Dose Response of Weed Seeds, Plant-Parasitic Nematodes, and Pathogens to Twelve Rates of Metam Sodium in a California Soil

Metam sodium (sodium N-methyl dithiocarbamate, metam-Na) is widely used in agricultural and floricultural production for controlling soilborne plant pathogens, parasitic nematodes, and weeds. It undergoes rapid decomposition to the biocide methyl isothiocyanate (MITC) in moist soils. In this study, the efficacy of 12 concentrations of metam-Na (10 to 2,650 μmol kg-1 soil) to control seeds or tubers of five major weed species, three soilborne pathogens, and one parasitic nematode was evaluated in a sandy loam soil under controlled conditions. Soils were exposed to the fumigant in microcosms for 24 h at 10 and 20°C. Generation and dissipation curves of MITC in soil under controlled conditions showed that MITC concentrations in soils were highest 2 h after metam-Na application and decreased steadily over the 24-h incubation period. After 24 h, remaining MITC concentrations in soil microcosms at 10 and 20°C were 53 and 38% of the original amount applied, respectively, indicating a 20% reduction in MITC dissipation at the lower soil temperature. Logistic dose-response models were used to estimate the effective concentration necessary to reduce soil pest viability by 50 (LC50) or 90 (LC90) percent under both temperatures. Seed of Portulaca oleracea, with LC90 values of ≤1,242 μmol kg-1 soil, was the most sensitive to soil fumigation with metam-Na, followed by Polygonum arenastrum with LC90 values of ≤1,922 μmol kg-1 soil. At 10°C fumigation temperature, metam-Na at the highest dose tested in this study, 2,650 μmol kg-1 soil, was not sufficient to achieve adequate control of Stellaria media and Malva parviflora seed and Cyperus esculentus tubers. Weed control efficacy (average reduction in LC90 values) of metam-Na was between 25 and 60% higher if soils were fumigated at 20°C compared with 10°C, with the exception of M. parviflora. Phytophthora cactorum and Pythium ultimum were more sensitive to soil fumigation with metam-Na (LC90 ≤ 165 μmol kg-1 soil) than Verticillium dahliae (LC90 ≤ 737 μmol kg-1 soil). The nematode Tylenchulus semipenetrans was highly sensitive to soil fumigation with metam-Na (LC90 ≤ 98 μmol kg-1 soil), and the efficacy of control increased by 30% if soil was fumigated at 20°C compared with 10°C. In this sandy loam soil, metam-Na at a concentration of 850 μmol kg-1 reduced the viability of Portulaca oleracea and Polygonum arenastrum seeds, C. esculentus tubers, and all soilborne pathogens and parasitic nematodes tested by 90% at 20°C after 24 h exposure. These results indicate that metam-Na can provide effective pest and disease control at maximum label rate for the commercial formulation, but there was a reduction in efficacy at low temperature.

Perennial Crop Nurseries Treated with Methyl Bromide and Alternative Fumigants: Effects on Weed Seed Viability, Weed Densities, and Time Required for Hand Weeding

Data on the efficacy of alternative fumigants to methyl bromide for weed control in perennial crop nurseries in California are needed because few herbicides are registered for this purpose. Field studies were conducted from 2003 to 2006 in four commercial perennial crop nurseries in California. Treatments included a nonfumigated control; methyl bromide (98%) (MeBr) with high-density polyethylene (HDPE) film; iodomethane (50%) + chloropicrin (50%) with HDPE film; 1,3-dichloropropene (1,3-D) with HDPE film; 1,3-D (61%) + chloropicrin (35%) with HDPE film; 1,3-D (62%) + chloropicrin (35%) subsurface drip; and 1,3-D (61%) + chloropicrin (35%) with virtually impermeable film (VIF). All the fumigants reduced the seed viability of common purslane, johnsongrass, and tall morningglory but were not as effective on little mallow and field bindweed. Although total weed densities and the level of control provided by each fumigant differed between locations, weed density was generally reduced by all the fumigation treatments, compared to the nonfumigated control. At three locations, alternative fumigation treatments usually resulted in hand-weeding time similar to MeBr. Reductions in weed seed viability, weed emergence, and weed densities suggest that these alternative fumigants will provide weed control similar to MeBr in perennial nurseries. Nomenclature: 1,3-dichloropropene, chloropicrin (trichloronitromethane), iodomethane, methyl bromide, common purslane, Portulaca oleracea L. POROL, field bindweed, Convolvulus arvensis L. CONAR, little mallow, Malva parviflora L. MALPA, johnsongrass, Sorghum halepense (L.) Pers. SORHA, tall morningglory, Ipomoea purpurea (L.) Roth PHBPU

Site--Specific Fumigant Applicator for Prevention of Almond Replant Disease

The goal of this research was to use recent advances in the global positioning system and computer technology to apply just the right amount of fumigant (0.2 kg/tree) where it is most needed (i.e., in the neighborhood of each tree planting site) to decrease the incidence of replant disease, and achieve the environmental and economical benefits of reducing the application of these toxic chemicals. In this study we have retrofitted a chemical applicator with a high--performance global positioning system receiver (accuracy in the range of 10 to 20 cm) and developed software necessary to accomplish tree--planting--site--specific application of fumigants. The results of accuracy tests indicated that the error in position location depended on vehicle speed and look--ahead value. A look--ahead value of 0.8 s appears to account for the hydraulic system response time. The RMS error was 33.5 cm (13.2 in) when the speed effect was properly accounting for. Although this error is higher than desirable, even at this level of accuracy the amount of fumigant applied can be reduced by nearly 50% thus realizing significant cost and environmental benefits.

Weed Community Composition in Tree Fruit Nurseries Treated with Methyl Bromide and Alternative Fumigants

Many agricultural cropping systems have relied on methyl bromide (MeBr) for pest control, including weeds, for decades. Alternative fumigants are being sought worldwide because MeBr has been identified as an ozone-layer depleting substance. Weed communities respond dynamically to alterations in management systems. Thus, transition from MeBr to alternative fumigants may cause shifts in weed communities. This hypothesis was tested in four commercial fruit nurseries in California, USA. Treatments included nonfumigated control, MeBr (98%), iodomethane (50%) + chloropicrin (50%), 1,3-dichloropropene (1,3-D), 1,3-D (61%) + chloropicrin (35%), and 1,3-D (62%) + chloropicrin (35%) applied subsurface. All the fumigants reduced the population of common major weed species and had similar species composition as MeBr. None of the fumigants, including MeBr, controlled species such as Medicago polymorpha, Lotus purshianus, Malva parviflora, Conyza sp., Senecio vulgaris, and Sonchus oleraceus. This study suggested that, fruit nurseries transitioning from MeBr to alternatives may not see an immediate shift in weed communities. However, Additional weed control measures will be required to manage weed species of the Asteraceae, Fabaceae, and Malvaceae family that are not controlled by either MeBr or the alternate fumigants.

Planting Site-Specific Application of Fumigant in Orchards

The goal of this research was to use recent advances in the global positioning system and computer technology to apply just the right amount of fumigant where it is most needed (i.e., in the neighborhood of each tree planting site or tree-planting-site-specific application) to decrease the incidence of replant disease, and achieve the environmental and economical benefits of reducing the application of these toxic chemicals. In the first year of this study we retrofitted a chemical applicator with a high-performance global positioning system receiver (accuracy in the range of 10 to 20 cm), an embedded controller to read GPS data and control a solenoid valve to implement tree-planting-site-specific fumigant application. Although the system worked well, the results of accuracy tests indicated that the RMS error in position location was 33.5 cm, which was more than desirable. To improve the position location accuracy, a new system was developed during the second year of this study. In this system, the embedded controller which was slow to perform all the necessary computations in real-time was replaced with a higher speed controller and used a Pulse Width Module (PWM) and solenoid actuated nozzles to provide precision rate on demand. Extensive testing indicated that the new system had a RMS error of less than 15 cm. The system was field tested in three almond orchards in California during Fall 2007. The system performed well in all three locations.

First Report of Phytophthora cinnamomi Causing Trunk Canker of Almond in California

Almond (Prunus dulcis) is the most economically important nut crop in California, accounting for

Dose response of weed seeds and soilborne pathogens to 1,3-D and chloropicrin

5.9 billion value in 2016. During fall of 2015, declining 2-year-old almond trees on clonal Hansen 536 (peach × almond) rootstock were observed in a commercial orchard in Kern County, California. Affected trees expressed early defoliation with amber-colored gummosis on the trunk. Cankers developed at the bud union and extended into the scion in trees planted with their bud union placed at or below the soil surface. To isolate the causal agent, necrotic pieces of bark and subtending vascular tissue were cut from the canker margin, surface sterilized in 0.5% sodium hypochlorite solution for 3 min, rinsed twice in sterile water, and plated on corn meal agar amended with selective antibiotics (PARP) (Jeffers and Martin 1986). After several days of incubation at 25°C, Phytophthora-like colonies grew from the diseased tissues and were transferred onto acidified potato dextrose agar medium (APDA). The isolates were tentatively identified as Phytophthora cinnamomi Rands based on morphological characteristics. Sporangia were nonpapillate and ovoid to obpyriform or ellipsoidal to elongate-ellipsoid with an apical thickening, avg. 57.2 × 36.6 µm with a length-width ratio of 1.56; mycelium with abundant coralloid hyphal swellings. Globose chlamydospores formed abundantly in culture on the parent hyphae or on new hyphal branches (18 to 42 [avg. 27.65] μm in diameter) (Erwin and Ribeiro 1996). For all isolates, the internal transcribed spacer (ITS) regions of the rRNA gene and a portion of the β-tubulin (TUB) gene were amplified and sequenced with primers ITS1/ITS4 and TUBUF2/TUBUR1 (Lan et al. 2016), respectively. Two representative sequences for each locus were deposited in GenBank (ITS; KY924650 and KY924651, TUB; KY924648 and KY924649). BLASTn analysis against Phytophthora Database (www.phytophthoradb.org) showed that the ITS sequences had 99% identity and the β-tubulin sequences had 97 to 98% identity to reference sequences of P. cinnamomi (ITS: GU259227; TUB: EU079894). Two of the almond isolates of P. cinnamomi were tested for pathogenicity by wound inoculating 2-year-old potted almond trees of cv. Nonpareil on rootstocks of Hansen 536 and Nemaguard (Prunus persica × Prunus davidiana). Five-millimeter-diameter mycelial disks from PDA cultures of each isolate (and sterile PDA as a control) were used for inoculating wounds of the same size (5 mm) made in stems of: i) the almond scion on Hansen 536 rootstock, ii) the almond scion on Nemaguard rootstock, iii) Hansen 536 rootstock below the scion, and iv) Nemaguard rootstock below the scion. Five replicate trees were inoculated per combination of the three inoculants (two of P. cinnamomi, one control) and the four inoculation treatments in a randomized complete block design. After inoculation, the wounds were covered with petroleum jelly and wrapped with Parafilm. Two months after inoculation, all plants inoculated with the control remained healthy, whereas all scions and rootstocks inoculated with either isolate developed stem cankers. None of the trees inoculated on the rootstocks died during the experiment, but 40% of the trees inoculated on the scion were killed. P. cinnamomi was reisolated only from the infected plants. More than 10 other species of Phytophthora were reported to cause root and crown rots as well as aerial cankers on almond (Browne and Viveros 1999; Browne et al. 2015). To our knowledge, this is the first report of P. cinnamomi causing trunk canker disease of almond in California.