Gregory W. Auner

Deposition factors and band gap of zinc-blende AlN

Successful deposition of zinc-blende AlN films with thickness up to 1000 A was performed with plasma source molecular beam epitaxy. The films were epitaxial to the Si(001) substrate. The formation of a thin 3C-SiC layer on the Si(001) surface is one of the important factors for the formation of zinc-blende AlN. Evidence for the presence of 3C-SiC is provided by an Auger electron spectroscopy depth profile and a high-resolution transmission electron microscopy plot profile. Spectroscopic ellipsometry was used to determine the optical constants of zinc-blende AlN in the range from 1.85 to 6.5 eV. The extinction coefficient data indicates that zinc-blende AlN is an indirect semiconductor with a band gap of ∼5.34 eV.

TEMPERATURE DEPENDENCE OF CONVENTIONAL AND EFFECTIVE PYROELECTRIC COEFFICIENTS FOR COMPOSITIONALLY GRADED BAXSR1-XTIO3 FILMS

Ferroelectric thin films (∼1.2 μm) of BaxSr1−xTiO3 with gradients in composition normal to the growth surface were formed on platinum substrates by metalorganic decomposition. Effective (pseudo) pyroelectric coefficients as large as 0.06 μC/cm2 K have been obtained from these active ferroelectric devices under the application of an ac field (charge pumping). In contrast, a value of only −0.003 μC/cm2 K has been measured for the conventional pyroelectric coefficient.

A simple miniature optical spectrometer with a planar waveguide grating coupler in combination with a plano-convex lens

A miniature optical spectrometer with a thin-film planar waveguide grating coupler in combination with a miniature plano-convex focusing lens has been investigated. With optical part of the spectrometer as small as 0.2 cubic cm, the spectral resolution varies from 0.3 nm to 4.6 nm within the wavelength range 488.0 nm - 632.8 nm.

Stress-induced polarization-graded ferroelectrics

Polarization-graded ferroelectrics and their electrically active embodiments, graded ferroelectric devices and transpacitors, have been formed from a variety of material systems, both by grading the composition of the ferroelectric and by imposing temperature gradients normal to the electrode surfaces. In this letter, we show how these same devices can be formed from homogeneous ferroelectric films of lead strontium titanate by imposing stress gradients on the material normal to their electrode surfaces.

Diagnosis of neuroblastoma and ganglioneuroma using Raman spectroscopy.

BACKGROUND Raman spectroscopy has proven to be useful in studying premalignant and malignant lesions in adults. This is the first report to evaluate Raman spectroscopy in the diagnosis and classification of neuroblastoma in children. METHODS A biopsy or resection of fresh tissue samples from normal adrenal glands, neuroblastomas, ganglioneuromas, nerve sheath tumors, and pheochromocytoma at our hospital were equally divided between routine histology and spectroscopic studies. At least 12 spectra were collected from different regions of each sample using a Renishaw Raman microscope. Raw spectra were processed to remove noise, fluorescence, and shot noise, and then analyzed using principle component analysis and discriminant function analysis. RESULTS We collected 698 spectra from 16 neuroblastomas, 5 ganglioneuromas, 3 normal adrenal glands, 6 nerve sheath tumors, and 1 pheochromocytoma. Raman spectroscopy differentiated between normal adrenal gland, and neuroblastoma and ganglioneuroma with 100% sensitivity and 100% specificity. It correlated well with the Shimada histologic classification system with 100% sensitivity and 100% specificity. It was also able to differentiate neuroblastoma from nerve sheath tumors and pheochromocytoma with high sensitivity and specificity. CONCLUSION This technique can differentiate neuroblastoma from ganglioneuroma and other tumors. It has a potential as a noninvasive real-time diagnostic tool in classifying pediatric tumors.

Concept of a miniature optical spectrometer using integrated optical and micro-optical components.

We describe the concept of a super compact diffractive imaging spectrometer, with optical components a few millimeters across in all dimensions, capable of detecting optical fluorescence spectra within the entire visible spectral range from 400 nm to 700 nm with resolution of the order of 2 nm. In addition, the proposed spectrometer is capable of working simultaneously with multiple, up to 35, independent input optical channels. A specially designed diffractive optical element integrated with a planar optical waveguide is the key component of the proposed device. In the preliminary experimental tests, a uniform waveguide grating with a microlens was used to mimic operation of the diffractive optical element. A microspectrometer with optical components measured below 1 cm in all dimensions covers the spectral range from 450 nm to 650 nm and shows a spectral resolution of 0.5 nm at wavelengths close to 514 nm and 633 nm.

Emerging technology: applications of Raman spectroscopy for prostate cancer.

There is a need in prostate cancer diagnostics and research for a label-free imaging methodology that is nondestructive, rapid, objective, and uninfluenced by water. Raman spectroscopy provides a molecular signature, which can be scaled from micron-level regions of interest in cells to macroscopic areas of tissue. It can be used for applications ranging from in vivo or in vitro diagnostics to basic science laboratory testing. This work describes the fundamentals of Raman spectroscopy and complementary techniques including surface enhanced Raman scattering, resonance Raman spectroscopy, coherent anti-Stokes Raman spectroscopy, confocal Raman spectroscopy, stimulated Raman scattering, and spatially offset Raman spectroscopy. Clinical applications of Raman spectroscopy to prostate cancer will be discussed, including screening, biopsy, margin assessment, and monitoring of treatment efficacy. Laboratory applications including cell identification, culture monitoring, therapeutics development, and live imaging of cellular processes are discussed. Potential future avenues of research are described, with emphasis on multiplexing Raman spectroscopy with other modalities.

Optical characterization of AlN films grown by plasma source molecular beam epitaxy

Thin films of AlN were grown on Si(111), sapphire (1102), and sapphire (0001) substrates by plasma source molecular beam epitaxy (PSMBE). Optical transmission spectra of PSMBE grown AlN/sapphire show neither significant band edge distortions nor deep level absorption in the spectral range 0.2–6.5 μm. Furthermore, there is no evidence of deep levels in the spectra of AlN/Si in the range 2.5–25 μm. The optical phonon modes of AlN/Si films were directly observed in infrared transmission and 80° angle of incidence reflectance spectra. Longitudinal optical (900 cm−1) and transverse optical (680 cm−1) modes are shifted toward each other comparable to previously reported bulk values. Optical spectra of thick textured film samples with low degree of orientation have phonon energies close to bulk values (probably due to strain relaxation).

Raman spectroscopic differentiation of activated versus non-activated T lymphocytes: an in vitro study of an acute allograft rejection model.

Acute rejection (AR) remains a significant complication in renal transplant patients. Using serum creatinine for AR screening has proven problematic, and thus a noninvasive, highly sensitive and specific test is needed. T cells from human peripheral blood were analyzed using Raman spectroscopy. Fifty-one Mixed Lymphocyte Culture (MLC) activated T cells (ATC), 28 Mitomycin C inactivated T cells (ITC), and 35 resting T cells (RTC), were studied utilizing 785 and 514.5 nm wavelengths. Statistical analysis following subtraction of fluorescence used Students t test to quantify peak ratio differences and discriminant function analysis (DFA), with three distinct sectors assigned for grouping purposes: Sector I, ITC; Sector II, ATC; Sector III, RTC. Differences between ATC and non-activated T cells (ITC and RTC) were found at 1182 and 1195 cm-1 peak positions for both wavelengths. Significant differences in peak ratios for 785 and 514.5 nm wavelengths existed between ATC and RTC (p=0.001 and p=0.006, respectively) and ATC and ITC (p=0.001 and p=0.001, respectively), with a trend in differences observed between ITC and RTC (p=0.07 and p=0.08, respectively). Analysis of the DFA-derived sector distribution for the 785 and 514.5 nm wavelengths revealed a sensitivity of 95.7% and 89.3%, respectively, and a specificity of 100% and 93.8%, respectively. This data suggests that Raman spectroscopy can detect significant differences between activated and nonactivated T cells based upon cell-surface receptor expression, thereby establishing unique signatures that can aid in the development of a noninvasive AR screening tool with high sensitivity and specificity.

Nanopatterning effects on astrocyte reactivity.

An array of design strategies have been targeted toward minimizing failure of implanted microelectrodes by minimizing the chronic glial scar around the microelectrode under chronic conditions. Current approaches toward inhibiting the initiation of glial scarring range from altering the geometry, roughness, size, shape, and materials of the device. Studies have shown materials which mimic the nanotopography of the natural environment in vivo will consequently result in an improved biocompatible response. Nanofabrication of electrode arrays is being pursued in the field of neuronal electrophysiology to increase sampling capabilities. Literature shows a gap in research of nanotopography influence in the reduction of astrogliosis. The aim of this study was to determine optimal feature sizes for neural electrode fabrication, which was defined as eliciting a nonreactive astrocytic response. Nanopatterned surfaces were fabricated with nanoimprint lithography on poly(methyl methacrylate) surfaces. The rate of protein adsorption, quantity of protein adsorption, cell alignment, morphology, adhesion, proliferation, viability, and gene expression was compared between nanopatterned surfaces of different dimensions and non-nanopatterned control surfaces. Results of this study revealed that 3600 nanopatterned surfaces elicited less of a response when compared with the other patterned and non-nanopatterned surfaces. The surface instigated cell alignment along the nanopattern, less protein adsorption, less cell adhesion, proliferation and viability, inhibition of glial fibrillary acidic protein, and mitogen-activated protein kinase kinase 1 compared with all other substrates tested.