Guangqin Zhu

Polymorphism of Exon 2 of FShβ Gene and Its Relationship with Reproduction Performance in Two Goat Breeds

Abstract The objectives of the present study were to detect the polymorphism in follicle stimulating hormone beta (FSHβ) gene and investigate the relationship between FSHβ gene and high prolificacy in goats. According to the sequence of ovine FSHβ gene, two pairs of primers were designed to detect single nucleotide polymorphism of exon 2 of FSHβ gene in two goat breeds, Xinong Saanen dairy goat and Boer goat, by PCR-SSCP. The least square mean and genetic variance of different genotypes at polymorphic locus were analyzed, and the selection reaction was forecasted by genetic correlation between marker locus and genetic variance of litter size traits. There was one polymorphic locus occurring in exon 2 (P2) with three genotypes (EE, EF and FF). In Xinong Saanen dairy goat, EE genotype had significantly higher (P

Screening of seven microsatellite markers for litter size in Xinong Saanen dairy goat

Seven microsatellite markers OarAE101, BM1329, OarHH55, BM143, BMS2508, OarAE129 and OarFCB11 closely associated with high reproduction trait in sheep were analyzed for polymorphisms in Xinong Saanen dairy goat. The results indicated that there were high genetic polymorphisms at six microsatellite loci. The number of effective alleles (Ne), polymorphism information content (PIC) and average heterozygosity (He) were the highest at OarFCB11 and the lowest at OarAE129 in Xinong Saanen dairy goat. The analysis of the effect of the six polymorphisms microsatellite loci on the litter size of Xinong Saanen dairy goat indicated that these polymorphisms microsatellite loci had positive effect on the litter size. Key words: Microsatellite markers, Xinong Saanen dairy goat, genetic polymorphism, litter size.

Polymerisation effects of four microsatellites on litter size in Xinong Saanen goats

In this study, the polymerisation effects of four microsatellites (OarAE101, BM1329, BM143 and LSCV043) on litter size in Xinong Saanen goats were analysed by means of microsatellite marker and pedigrees, then associations between combined genotypes and litter size were performed. The results indicate that the individuals with A5A1B10B5C5C1D6D2 (3.10 ± 0.07) had greater litter sizes than those with other combined genotypes in terms of average parity (P < 0.05). Comparing A5A1B10B5C5C1D6D2 with A7A2B10B5C5C1D6D2, the polymerisation effect value of the A5A1 genotype litter size was 18.09% higher than that of the A7A2 genotype. Comparing A5A1B6B1C6C1D6D2 of the F1 generation with A5A1B6B1C7C3D6D2 of the F2 generation, it was shown that the polymerisation effect value of the C6C1 genotype was 37.93% higher than that of the C7C3 genotype. Comparing A5A1B6B1C8C4D4D1 of the F1 generation with A5A1B6B1C8C4D9D5 of the F2 generation, it was shown that the polymerisation effect value of the D4D1 genotype was 68.07% higher than that of the D9D5 genotype. These results suggested that A5A1B10B5C5C1D6D2 is a useful marker affecting caprine litter size.