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Featured researches published by Guanlin Guo.


Bioresource Technology | 2012

Effect of biostimulation on community level physiological profiles of microorganisms in field-scale biopiles composed of aged oil sludge

Xiang Wang; Qunhui Wang; Shijie Wang; Fasheng Li; Guanlin Guo

Four biopiles were constructed for the bioremediation of total petroleum hydrocarbons (TPHs) in aged oily sludge by indigenous microorganisms. Three biopiles were treated with bulking agent, nutrients or their combination with the fourth pile serving as the control. Responses of microbial community level physiological profiles (CLPPs) to these biostimulation strategies were estimated, and variances among three layers within each biopile were evaluated. Microbial metabolic activity and diversity and the numbers of two species of bacteria were significantly enhanced by the addition of bulking agent, which also made the layers more homogeneous. In contrast, the application of large amounts of nutrients had a suppressing effect on the microbes. After 220 days, 49.62% of TPHs were removed from the middle layer of the bulking agent pile, whereas only 20.44% were removed from the inner layer of the control. This study aims to provide experience to improve the remediation efficiency of future investigations.


Journal of Chromatography A | 2010

The development of a method for the qualitative and quantitative determination of petroleum hydrocarbon components using thin-layer chromatography with flame ionization detection.

Shijie Wang; Guanlin Guo; Zengguang Yan; Guilan Lu; Qunhui Wang; Fasheng Li

An analytical scheme to determine groups of petroleum hydrocarbon compounds in crude oil was developed and used for the qualitative and quantitative characterization of crude oil samples from the Shengli oilfield, the second largest oilfield in China. Crude oil samples were fractionated and analyzed by thin-layer chromatography with flame ionization detection (TLC-FID). Relative standard deviation (RSD) values for retention time, peak height and half peak width were less than 5.2% for all classes of compounds, based on nine independent replicates. The crude oil light fraction was further analyzed by GC-MS and the majority of identified compounds were methyl- or hydro-derivatives of long-chain hydrocarbons and aromatic compounds. The external standard method used in the present study can lower detection limits of petroleum hydrocarbon compound classes to 20.0 mg L(-1), and the crude oil concentration in the range of 30 and 35,000 mg L(-1) has a high linear correlation (r(2)>0.97, P<0.05) with peak area. A comparison between elution chromatography (EC) and TLC-FID regarding the recovery of petroleum hydrocarbon compounds was carried out with aged crude oil contaminated soils of 50, 80, 200 and 300 mg g(-1). The tested TLC-FID method showed a 10% higher recovery for total extractable materials than the reference EC method. The calibration factor was fraction-dependent and varied with the recovery rate of TLC/EC. Regarding the tested extraction procedures, accelerated solvent extraction (ASE) had a higher extraction efficiency for crude oil contaminated soils than Soxhlet and ultrasonic extractions.


Environmental Monitoring and Assessment | 2010

Ecotoxicity assessment of aged petroleum sludge using a suite of effects-based end points in earthworm Eisenia fetida

Shijie Wang; Zengguang Yan; Guanlin Guo; Guilan Lu; Qunhui Wang; Fasheng Li

Laboratory investigations were conducted to test the toxicity of aged petroleum sludge collected from Shengli Oil Field, the second largest oilfield in China, to earthworm Eisenia fetida. Various end points were measured in the earthworms, including mortality, growth, cocoon output, juvenile production, and avoidance behavioral response, to determine their comparative sensitivity for assessing harmful effects of petroleum-hydrocarbon-contaminated soil. The results showed that all these assays responded in a concentration-dependent manner, and two chronic end points, juvenile production and cocoon output, as well as avoidance behavioral response appeared to be sensitive end points for detecting toxicity of petroleum-hydrocarbon-contaminated soil. Comparatively, juvenile production exhibited similar sensitivity to avoidance behavior response, both of which were more sensitive than cocoon output, while mortality and adult growth were proposed as the least sensitive parameters. It was suggested that large amounts of petroleum sludge deposited in Shengli Oil Field may pose a potential threat to the local ecosystem, and the utility of multiple effects-based end points in earthworm E. fetida is useful to facilitate ecological risk assessments in hydrocarbon-contaminated sites.


Environmental Toxicology and Chemistry | 2011

Uptake and toxicity of spiked nickel to earthworm Eisenia fetida in a range of Chinese soils

Zengguang Yan; Beixin Wang; Dongli Xie; Youya Zhou; Guanlin Guo; Meng Xu; Liping Bai; Hong Hou; Fasheng Li

Bioavailability and toxicity of metals to soil organisms varies among different soils, and knowledge of this variance is useful for the development of soil environmental quality guidelines. In the present study, laboratory experiments were performed to investigate the effects of variations in nickel (Ni) uptake and toxicity on growth, cocoon output, and juvenile production in the earthworm Eisenia fetida in 13 Chinese soils spiked with nickel chloride. Body weight development of E. fetida was rather insensitive to Ni, and significant inhibition of growth was observed only at high Ni concentrations, such as 560 and 1000 mg/kg. The 50% inhibition effect concentrations (EC50s) for cocoon and juvenile production, based on measured Ni concentrations in soils, varied from 169 to 684 mg/kg and from 159 to 350 mg/kg, respectively. The EC50s represented approximately fourfold variation for cocoon output and twofold variation for juvenile production among 13 Chinese soils. Juvenile production, compared to cocoon output, was a more sensitive endpoint parameter to Ni. Nickel uptake in E. fetida increased as simple linear functions of increasing soil Ni concentrations. Tissue Ni-based EC50s (based on Ni concentrations in earthworm tissues) for cocoon production varied from 37 to 121 mg/kg (threefold variation) in 12 of 13 soils, suggesting a similar variation to that of soil Ni-based EC50s. Relationship analysis between soil properties and Ni toxicity showed that neither the EC50s for cocoon output nor those for juvenile production presented significant correlation with soil properties (pH, organic matter content, cation exchange capacity, clay content, Ca²⁺ and Mg²⁺). This may be ascribed to the narrow range of properties of selected soils. The soil factors that determined Ni toxicity to earthworm reproduction remain undetermined in the present study, and these data should be used cautiously when developing toxicity prediction models because of the narrow selection of soil properties.


Science of The Total Environment | 2013

Health and ecological risk-based characterization of soil and sediment contamination in shipyard with long-term use of DDT-containing antifouling paint

Guanlin Guo; Chao Zhang; Guanglong Wu; Shijie Wang; Fasheng Li

Dichlorodiphenyltrichloroethane (DDT) was a frequently occurring type of persistent organic environmental pollutant in China and DDT-containing antifouling paint could be the main contributor of DDT to shipyards and fishing harbors. A field survey was conducted in a shipyard in southern China to investigate the content and distribution of DDT in soil and sediments. Human health and screening-level ecological risk assessments were conducted for DDT contamination in soil and sediments and the results indicated that total DDT in all samples tested exceeded present advisory safe limits. Analysis of the composition and distribution implicated DDT-containing antifouling paint used for ship maintenance as an important source of DDT. Individual and cumulative health risks for residents exceeded the extra lifetime cancer risks of 10(-6) and 10(-5), mainly from exposure to soil, ingestion and dermal contact. DDT in sediments is associated with a high level of toxicity for the benthic community when >99% of samples exceed the threshold concentration likely to be responsible for effects and severe effects. Further risk control for DDT is required to ensure safety for human health, the benthic community and the environment.


Environmental Science and Pollution Research | 2015

Accuracy and uncertainty analysis of soil Bbf spatial distribution estimation at a coking plant-contaminated site based on normalization geostatistical technologies.

Geng Liu; Junjie Niu; Chao Zhang; Guanlin Guo

Data distribution is usually skewed severely by the presence of hot spots in contaminated sites. This causes difficulties for accurate geostatistical data transformation. Three types of typical normal distribution transformation methods termed the normal score, Johnson, and Box–Cox transformations were applied to compare the effects of spatial interpolation with normal distribution transformation data of benzo(b)fluoranthene in a large-scale coking plant-contaminated site in north China. Three normal transformation methods decreased the skewness and kurtosis of the benzo(b)fluoranthene, and all the transformed data passed the Kolmogorov–Smirnov test threshold. Cross validation showed that Johnson ordinary kriging has a minimum root-mean-square error of 1.17 and a mean error of 0.19, which was more accurate than the other two models. The area with fewer sampling points and that with high levels of contamination showed the largest prediction standard errors based on the Johnson ordinary kriging prediction map. We introduce an ideal normal transformation method prior to geostatistical estimation for severely skewed data, which enhances the reliability of risk estimation and improves the accuracy for determination of remediation boundaries.


Environmental Geochemistry and Health | 2016

Characterization and assessment of contaminated soil and groundwater at an organic chemical plant site in Chongqing, Southwest China

Geng Liu; Junjie Niu; Chao Zhang; Guanlin Guo

Contamination from organic chemical plants can cause serious pollution of soil and groundwater ecosystems. To characterize soil contamination and to evaluate the health risk posed by groundwater at a typical organic chemical plant site in Chongqing, China, 91 soil samples and seven groundwater samples were collected. The concentrations of different contaminants and their three-dimensional distribution were determined based on the 3D-krige method. Groundwater chemistry risk index (Chem RI) and cancer risk were calculated based on TRIAD and RBCA models. The chemistry risk indices of groundwater points SW5, SW18, SW22, SW39, SW52, SW80, and SW82 were 0.4209, 0.9972, 0.9324, 0.9990, 0.9991, 1.0000, and 1.0000, respectively, indicating that the groundwater has poor environmental status. By contrast, the reference Yangtse River water sample showed no pollution with a Chem RI of 0.1301. Benzene and 1,2-dichloroethane were the main contaminants in the groundwater and were responsible for the elevated cancer risk. The cumulative health risk of groundwater points (except SW5 and SW18) were all higher than the acceptable baselines of 10−6, which indicates that the groundwater poses high cancer risk. Action is urgently required to control and remediate the risk for human health and groundwater ecosystems.


Archive | 2018

Progress in the Risk Management of Contaminated Sites: Research Activities and Environmental Management of Contaminated Sites at CRAES

Qingbao Gu; Guanlin Guo; Jin Ma; Youya Zhou; Zengguang Yan; Yunfeng Xie; Bing Yang; Li Liu; Hong Hou; Xiaoming Du; Nandong Xue; Yunzhe Cao; Fujun Ma; Ping Du; Liping Bai; Fasheng Li

The issue of contaminated sites is receiving increasing public concern in China due to its close relation with industrial activities, disposal of hazardous materials, and environmental accidents. CRAES has conducted many successful studies of soil pollution and remediation. In this chapter, six sections are included to briefly introduce these studies. Section 1 – “Introduction” – describes the background of risk management of contaminated sites. Section 2 – “Site Investigation and Risk Assessment” – describes the progress in pollution investigation and source appointment, risk assessment of contaminants and uncertainty analysis, and spatial pattern and distribution mapping of soil contaminants on certain sites. Section 3 – “Technologies Used for Soil Remediation and Risk Control” – describes the progress in bioremediation technology, thermal desorption, and mechanical soil aeration and stabilization and solidification. Section 4 – “The Environmental Fate and Criteria of Contaminants” – describes the progress in the vertical transport of PAHs in soils, the sorption kinetic processes of organic compounds in soils, the air-soil exchange of organochlorine pesticides, and the soil environmental criteria for antimony (Sb) based on groundwater protection. Section 5 describes the “Technical Support That CRAES Provided for Soil Environmental Management,” including national soil survey, soil pollution accident response, POPs-contaminated site management, and soil screening level establishment for central or local government. Section 6 – “Demonstrations and Projects” – provides a summary of demonstrations and projects that CRAES took part in. These demonstrations and projects include petroleum refinery site, oil fields, steel production site, medicine production site, pulp and paper production site, fertilizer production site, abandoned coking plant site, chloralkali production site, chromate plants, and pesticide plants. These studies have improved our understanding of soil pollution status and the development of remediation technologies in China and will aid in the formulation of policies and regulations regarding soil pollution control.


International Journal of Phytoremediation | 2016

Screening of herbaceous plants for peat-enhanced rehabilitation of contaminated soil with oily sludge

Shijie Wang; Chao Zhang; Guilan Lu; Fasheng Li; Guanlin Guo

abstract A batch pot experiment using nine herbaceous species were conducted for peat enhanced rehabilitation of contaminated soil with oily sludge in the initial contents of 0%, 1.3%, 7.4%, and 12.2%, respectively. The results showed that petroleum hydrocarbons removal, plant growth indices and enzyme activities varied depending on plant species and oil contents. Cotton, ryegrass and tall fescue were effective in the rehabilitation of oily sludge contaminated soils. The total petroleum hydrocarbon (TPH) removal ranged from 30.0% to 40.0% after 170 days of treatment. Plant biomass was shown to be the preferred indicator for screening phytoremediation plant because it was closely correlated with TPH removal and enzyme activities. Peat-enhanced plant rehabilitation could be a good strategy for the treatment of oily sludge contaminated saline soils.


Soil Biology & Biochemistry | 2014

Interactive effects between earthworms and maize plants on the accumulation and toxicity of soil cadmium

Yanli Du; Meng-Meng He; Meng Xu; Zengguang Yan; Youya Zhou; Guanlin Guo; Jing Nie; Lin-Quan Wang; Hong Hou; Fasheng Li

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Shijie Wang

University of Science and Technology Beijing

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Qunhui Wang

University of Science and Technology Beijing

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Xiang Wang

University of Science and Technology Beijing

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Geng Liu

Taiyuan Normal University

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Hong Hou

Tokyo University of Agriculture and Technology

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Junjie Niu

Taiyuan Normal University

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Beixin Wang

Nanjing Agricultural University

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Bing Yang

Sun Yat-sen University

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Dongli Xie

Nanjing Agricultural University

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