Guilherme Buzon Gregores

Myocardial homing after intrapericardial infusion of Bone Marrow Mononuclear Cells

Erika Branco1, Emerson Ticona Fioretto2, Rosa Cabral3, Carlos Alberto Sarmento Palmera4, Guilherme Buzon Gregores4, Angelo Joao Stopiglia4, Paulo Cesar Maiorka4, Pedro Alves Lemos5, Carlos Campos5, Celso Takimura5, Jose Antonio Franchini Ramires5, Maria Angelica Miglino4 Faculdade de Medicina Veterinaria da Universidade Federal Rural da Amazonia – UFRA1, Belem, PA; Universidade Federal do Sergipe – UFS2, Aracaju, SE; Universidade Feral do Piaui – UFPI3, Teresina, PI; Faculdade de Medicina Veterinaria e Zootecnia FMVZ/USP4, Sao Paulo, SP; Instituto do Coracao – Incor5, Sao Paulo, SP, Brasil

Cell Therapy for Fibrotic Interstitial Pulmonary Disease: Experimental Study

Parte superior do formulário Digite um texto ou endereço de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced‐disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High‐resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90‐days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180‐days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre‐existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech., 2011.

Chorion biopsy in mongrel dogs

With the great development of the gestational studies in all of the species, we noticed the necessity of adaptations of these techniques for prenatal diagnosis in dogs. Based on this, we studied the feasibility of chorion biopsy guided by ultrasound. Our results demonstrated accuracy on the sex determination being 2 males and 12 females, as well as it would be possible to identify chromosome alteration due to the quality of samplings. Sex determination was accomplished with the identification of Y gene chromosomes in PCR technique. After the collection, fragments were prepared for light microscopy studies and revealed fetal chorion tissue, blood colloid and erythrocyte. In the whole material we found hemosiderin impregnations due to the hemolysis and to the residue of blood of the placental marginal hematomes. The submitted female dogs to this technique demonstrated normal puppy births without death.

Morphological aspects of buffaloes (Bubalus bubalis) umbilical cord

Bufalo e uma importante fonte de recurso nos rebanhos animais, apresentando uma grande participacao na agropecuaria, provendo leite, carne e forca de trabalho. O Cordao umbilical e responsavel pela troca de nutrientes materno-fetais durante a gestacao, e suas alteracoes podem comprometer o desenvolvimento fetal. Nos investigamos dez uteros gravidicos de bufalos de racas cruzadas em fases diferentes de gestacao. O periodo de gestacao e a idade fetal foram determinados pelo comprimento apice sacral, aplicando formulas previamente estabelecidas. Posteriormente mediu-se o comprimento do cordao umbilical. A rede vascular do cordao umbilical e anastomoses foram observadas por injecao ou latex de neoprene. O cordao umbilical foi estudado a partir de cortes histologicos, corados por HE, picrossirius, azul de Toluidina, orceina e reacao histoquimica de PAS. O cordao umbilical de bufalos e formado por duas arterias centrais, ducto alantoide e duas veias perifericas e apresentam forma de ampulheta. A parede da arteria umbilical e composta por grande quantidade de fibras colagenas e elasticas, fibroblastos e um grande numero de vasa vasorum. O ducto alantoide fica alocado entre as arterias e apresenta um grande numero de pequenos vasos nutritivos. Os vasos nutritivos devem ser cuidadosamente identificados para evitar-se confundi-los com vasa vasorum. O comprimento medio do cabo de cordao umbilical dos bufalos era 11.8cm (minimo de 6.8cm e maximo de 17.4cm).

AVALIAÇÃO DA TÉCNICA DE ARTERIOGRAFIA CEREBRAL GUIADA POR FLUOROSCOPIA EM SUÍNOS

The cerebral arteriography is a radiologic exam for demonstration of arterial vascularization using an injection of positive contrast for visualization of blood supply. In this research six pigs were submitted to cerebral arteriography under general anesthesia and with the assistant of fluoroscopy the femoral artery was catheterized until the carotid common right artery using contrast under selective form. The aim of this work was to analyze this technique as a complementary method in cases of suspect of alterations of blood flow. This technique shows be effective to obtain nithid images and to determinate the cerebral irrigation.