Guillaume Villatte

Photoactive TiO2 antibacterial coating on surgical external fixation pins for clinical application

External fixation is a method of osteosynthesis currently used in traumatology and orthopedic surgery. Pin tract infection is a common problem in clinical practice. Infection occurs after bacterial colonization of the pin due to its contact with skin and the local environment. One way to prevent such local contamination is to create a specific coating that could be applied in the medical field. In this work, we developed a surface coating for external fixator pins based on the photocatalytic properties of titanium dioxide, producing a bactericidal effect with sufficient mechanical strength to be compatible with surgical use. The morphology and structure of the sol-gel coating layers were characterized using, respectively, scanning electron microscopy and X-ray diffraction. The resistance properties of the coating were investigated by mechanical testing. Photodegradation of acid orange 7 in aqueous solution was used as a probe to assess the photocatalytic activity of the titanium dioxide layers under ultraviolet irradiation. The bactericidal effect induced by the process was evaluated against two strains, ie, Staphylococcus aureus and multiresistant Staphylococcus epidermidis. The coated pins showed good mechanical strength and an efficient antibacterial effect after 1 hour of ultraviolet irradiation.

Opening-wedge high tibial osteotomy with a secure bone allograft (Osteopure™) and locked plate fixation: Retrospective clinical and radiological evaluation of 69 knees after 7.5 years follow-up

BACKGROUND Medial opening-wedge high tibial osteotomy (OWHTO) provides reliable and long-lasting benefits, despite the wide range of wedge-filling and internal-fixation techniques used. The purpose of this work was to assess the clinical and radiological outcomes in a case-series of OWHTO performed using a secure bone allograft and locked plate fixation. HYPOTHESIS The clinical and radiological outcomes of OWHTO with a high-safety bone allograft and locked plate fixation are similar to those reported in previous case-series studies. MATERIALS AND METHODS A single-centre retrospective design was used to study 69 knees in 64 patients with a mean age of 51.8years (31-53years) and a preoperative hip-knee-ankle (HKA) angle of 173° (165°-180°). The wedge was filled with secure Osteopure™ bone allograft and fixation was achieved using an Integra Surfix(®) locked plate. Mean follow-up was 7.5years (5-9.3years). Clinical and functional outcomes were assessed by determining the IKS and KOOS-PS scores and recording complications related or unrelated to the allograft. The main criterion for assessing OWHTO survival was the time to revision surgery for symptom recurrence. Radiological assessment criteria were the HKA angle, tibial slope, patellar height, and osteoarthritis grade. GESTO criteria were used to evaluate the behaviour of the allograft. RESULTS Of the 69 knees, 64 (92.8%) were re-evaluated. The survival rate after 9.3years was 95%±2.7% (3 failures managed with arthroplasty). The functional IKS score improved significantly, by 20 points (P<0.001). Mean increases of 7.8° for the HKA angle and 3.5° for tibial slope were recorded. Bone healing without radiological abnormalities was consistently achieved within 6months. There were no complications related to the allograft (infections, allergies; local or systemic toxicity). DISCUSSION The clinical, radiological, and safety outcomes documented in our study were similar to those reported in earlier work. LEVEL OF EVIDENCE IV, retrospective case-series study.

Collection and reconstruction after harvesting donor tissues from the musculoskeletal system: Technique specific to the lower limbs

The lack of available musculoskeletal grafts in France forces us to import a very large quantity of these tissues to use in complex reconstruction procedures. The goal of this article is to describe methods for collecting donor tissues from the musculoskeletal system and for reconstructing the harvested areas. We also provide a summary of the collection procedures performed, harvested grafts and available tissues. While tissue collection requires a significant time investment, the emergence of dedicated teams may be a solution for increasing the number and quality of human musculoskeletal allograft tissues.

Usefulness of sonication for diagnosing infection in explanted orthopaedic implants

BACKGROUND Orthopaedic implant infection is a rare but serious complication whose optimal treatment requires an accurate microbiological diagnosis. The objective of this study was to determine whether culturing sonicated explants improved sensitivity compared to culturing standard sonicated soft-tissue samples. HYPOTHESIS Cultures of explant sonication fluid are more sensitive than cultures of soft-tissue sonication fluid in patients with implant infection. METHODS This single-centre retrospective study included all sonication fluid samples from implants explanted in orthopaedic surgery theatres for any reason. The microbiological results of the implant sonication fluid cultures were compared to those of cultures of sonicated soft-tissue and bone samples taken during the same procedure. The primary evaluation criterion was the difference in microorganisms recovered from explant sonication fluids versus fluid/tissue cultures. RESULTS The study included 187 explants removed between September 2009 and June 2015. Of the definite infections, 83% were identified by explant sonication, 86% by fluid/tissue cultures, and 91% by both techniques combined. Explant sonication recovered causative organisms in 10 patients with definite infection but negative fluid/soft tissue cultures. Antibiotic therapy prior to explantation was associated with lower sensitivity of explant sonication (57% vs. 67% for fluid/soft tissue cultures). CONCLUSION Explant sonication improved the diagnosis of infection when combined with fluid/soft tissue cultures. LEVEL OF EVIDENCE IV, retrospective single-centre study.

Complications with cement spacers in 2-stage treatment of periprosthetic joint infection on total hip replacement

INTRODUCTION The aim of the present study was to assess our management of infected total hip replacement in indications for 2-stage surgical treatment according to current guidelines when a cement spacer has been used. The study hypothesis was that the complications rate related to cement spacers is low. MATERIAL AND METHOD A single-center retrospective study included 26 patients receiving a spacer between the two stages of total hip replacement, over a 5 year period. We analyzed the spacers used, mechanical complications, infectious complications and the second stage of surgery. RESULTS During the interval between surgeries, in the 26 patients, there were 19 spacer-related mechanical complications (73% of patients): 11 dislocations (42.3%), 5 spacer fractures (19.2%), 5 femoral bone lyses or fractures (19.2%) and 3 acetabular lyses or fractures (11.5%). In 4 cases, complications were associated. The greater the femoral offset of the spacer, the more frequent were femoral bone lyses or fractures (p=0.05), and the smaller the offset the more frequent were acetabular lyses or fractures (p=0.05). The rates of mechanical complications (p=0.003) and spacer fracture (p=0.02) were significantly greater in older patients. There were 4 cases of reinfection (19%): i.e., an 81% treatment success rate. One reinfection implicated a new bacterium: methicillin-susceptible Staphylococcus aureus. The second surgical stage was significantly longer in complex bipolar revision (p=0.009). CONCLUSION The present results showed a high risk of spacer-related complications, and thus the importance of selection of patients liable to derive real benefit and those for whom a Gilderstone procedure would be preferable. LEVEL OF EVIDENCE IV, retrospective study.

Use of Patient-Specific Instrumentation (PSI) for glenoid component positioning in shoulder arthroplasty. A systematic review and meta-analysis

Introduction Total Shoulder Arthroplasty (TSA) anatomical, reverse or both is an increasingly popular procedure but the glenoid component is still a weak element, accounting for 30–50% of mechanical complications and contributing to the revision burden. Component mal-positioning is one of the main aetiological factors in glenoid failure and thus Patient-Specific Instrumentation (PSI) has been introduced in an effort to optimise implant placement. The aim of this systematic literature review and meta-analysis is to compare the success of PSI and Standard Instrumentation (STDI) methods in reproducing pre-operative surgical planning of glenoid component positioning. Material and methods A search (restricted to English language) was conducted in November 2017 on MEDLINE, the Cochrane Library, EMBASE and ClinicalTrials.gov. Using the search terms “Patient-Specific Instrumentation (PSI)”, “custom guide”, “shoulder”, “glenoid” and “arthroplasty”, 42 studies were identified. The main exclusion criteria were: no CT-scan analysis results; studies done on plastic bone; and use of a reusable or generic guide. Eligible studies evaluated final deviations from the planning for version, inclination, entry point and rotation. Reviewers worked independently to extract data and assess the risk of bias on the same studies. Results The final analysis included 12 studies, comprising 227 participants (seven studies on 103 humans and five studies on 124 cadaveric specimens). Heterogeneity was moderate or high for all parameters. Deviations from the pre-operative planning for version (p<0.01), inclination (p<0.01) and entry point (p = 0.02) were significantly lower with the PSI than with the STDI, but not for rotation (p = 0.49). Accuracy (deviation from planning) with PSI was about 1.88° to 4.96°, depending on the parameter. The number of component outliers (>10° of deviation or 4mm) were significantly higher with STDI than with PSI (68.6% vs 15.3% (p = 0.01)). Conclusion This review supports the idea that PSI enhances glenoid component positioning, especially a decrease in the number of outliers. However, the findings are not definitive and further validation is required. It should be noted that no randomised clinical studies are available to confirm long-term outcomes.

Ten-year epidemiological study in an orthopaedic and trauma surgery centre: Are there risks involved in increasing scheduled arthroplasty volume without increasing resources?

BACKGROUND Current trends in patient management include decreasing hospital stay lengths and reductions in available material and human resources. A shortening of hospital stays in university hospitals has been documented over the last decade. However, to our knowledge, no study has assessed possible relationships linking shorter stays to staffing levels or complication rates. The objectives of this study were: 1) to assess changes in case volume in a university orthopaedics and trauma surgery department between 2006 and 2016, 2) and to look for correlations linking these changes to staffing levels and the rates of significant complications, including the number of hip dislocations after total hip arthroplasty (THA) and the number of infections and complications resulting in malpractice litigation after hip, knee, or shoulder arthroplasty. HYPOTHESIS The case volume increased during the study period, whereas resources remained unchanged or decreased. MATERIAL AND METHODS A retrospective study was performed using the electronic database of an orthopaedics and trauma surgery department. Data collected between 2006 and 2016 were analysed. Mean hospital stay length, patient age, and surgical volume were recorded, and changes over time in case volume for trauma surgery and scheduled arthroplasties were evaluated. Changes in staffing levels and rates of complications (dislocation after THA and infections and complications resulting in malpractice litigation) between 2006 and 2016 were assessed. Only arthroplasty procedures performed in the department were considered for the study of complications and litigation. RESULTS Between 2006 and 2016, mean hospital stay decreased from 8.7±10.8 days (range, 0-141 days) in to 7.0±9.4 days (range, 0-150 days). Mean patient age increased from 54.4±21.2 years (range, 11.7-100.9 years) in 2006 to 59.3±20.9 years (range, 13.2-103.1 years) in 2016. The total number of procedures rose from 2158 in 2006 to 3100 in 2016 (+43.6%). The number of THAs increased by 16.2% and the number of total knee arthroplasties by 96.7%. The number of operations for trauma increased from 725 in 2006 to 1135 in 2016 (+56.0%). During the study period, the number of hospital beds declined from 70 to 55. No increase was seen in the frequencies of dislocation after THA (3/284 [1.4%] in 2006 and 4/330 [1.2%] in 2016) or prosthetic joint infection (5/439 [1.1%] in 2006 and 6/657 [0.9%] in 2016). In contrast, malpractice suits filed by patients after arthroplasty increased from 1/439 (0.2%) in 2006 to 8/657 (1.2%) in 2016. CONCLUSION Over the last decade, trauma and arthroplasty surgical volumes increased substantially, whereas staffing levels remained unchanged and number of beds diminished. The frequency of significant complications such as dislocation after THA did not increase. In contrast, a marked rise was seen in malpractice litigation. However, the increased volume with unchanged resources found in this study should be interpreted in the light of the marked increase in patient dependency and of our role as a referral centre managing patients with complications after surgery performed at other institutions. LEVEL OF EVIDENCE IV, retrospective observational study.

Update on the supply and use of allografts in locomotor system pathologies in France

Allografts are increasingly used in orthopedics. The main aim of the present study was to map the use of locomotor system allografts in France between 2012 and 2016. The study hypothesis was that there are great differences in the distribution and activity of tissue banks and graft preservation procedure quality, failing to meet national requirements. MATERIAL AND METHODS Data from activity reports of the French Biomedicine Agency (ABM) were collected for the period 2012-2016. Existing viral inactivation procedures were described. Preliminary results from a study of allograft requirements by the French Society of Arthroscopy (SFA) were reported. RESULTS Nineteen tissue banks were located. Four dealt exclusively with cryopreserved tissue, 3 exclusively with virus-inactivated bone, and 12 with both. Distribution analysis found wide disparities in geographic location and in type of activity. Viral inactivation is presently implemented only for femoral heads derived from hip replacement. Stocks of long bones, femoral heads and ligaments/tendons increased constantly over the study period, by 8.3%, 50.8% and 316.2% respectively. The SFA questionnaire confirmed a serious shortage of tissues, necessitating importation of allografts. DISCUSSION Each tissue bank had its own specificities and specialization. They should probably be coalesced, so as to centralize both supply and demand and improve nationwide response to requirements. Locomotor system tissue harvesting also needs to be expanded to meet increasing demand.

Irradiation at 11 kGy conserves the biomechanical properties of fascia lata better than irradiation at 25 kGy

&NA; The objective of this study was to determine the biomechanical properties of the fascia lata and the effects of three preservation methods: freezing, cryopreservation with dimethylsulfoxide solution and lyophilization; and to compare the effects of low‐dose (11 kGy) and normal‐dose (25 kGy) gamma‐ray sterilization versus no irradiation. 248 samples from 14 fasciae latae were collected. Freezing samples were frozen at −80 °C. Cryopreservation with dimethylsulfoxide solution samples were frozen with 10 cl dimethylsulfoxide solution at −80 °C. Lyophilization samples were frozen at −22 °C and lyophilized. Each preservation group were then randomly divided into 3 irradiation groups. The cryopreservation with dimethylsulfoxide solution samples had significantly worse results in all 3 irradiation conditions. Youngs modulus was lower for the freezing samples (p < 0.001) and lyophilization samples groups (p < 0.001). Tear deformation was lower for the freezing samples (p = 0.001) and lyophilization samples groups (p = 0.003), as was stress at break (p < 0.001 and p < 0.001). Taking all preservation methods together, samples irradiated at 25 kGy had worse results than the 0 kGy and 11 kGy groups in terms of Youngs modulus (p = 0.007 and p = 0.13) and of stress at break (p = 0.006 and p = 0.06). The biomechanical properties of fascia lata allografts were significantly worse under dimethylsulfoxide cryopreservation. The deleterious effects of irradiation were dose‐dependent. HighlightsFirst study about 11 kGy applied to tendonsLarge possible clinical applicationLot of specimen tested

Endoscopic Hip Surgery in the Treatment of External Snapping Hip for A 22 Years Old Women

The snapping hip is a painful and disabling condition, related to a conflict between the greater trochanter and the soft tissue surrounding it. It is characterized by an audible click and sometimes visible on the thigh lateral region during the movements. The classical treatment is with opened surgery, but only few cases are reported. We present the case of a young 22 years old Caucasian female with external snapping hip and associated with tendinitis of the medium and maximus gluteus. The diagnosis was done clinically and radiologically with a nuclear magnetic resonance. The treatment was performed by a hip endoscopy after failure of medical treatment and physiotherapy. The bursectomy and the adhesions’s release gave very good results for our patient. Peritrochanteric endoscopy seems to have a place in the treatment of external snapping hip, allowing good visualization of lesions and optimal therapeutic management by first mini invasive routes. It might be a way for a better recover.