Gulfem Ece

The Evaluation of the Distribution of Yeast like Fungi 'Candida Species' at a Tertiary Care Center in Western Turkey

Objective: Candida infections have increased due to transplant patients, prolonged ICU stay and invasive procedures. The most common isolated strain is C. albicans. The aim of this study was to evaluate the distribution of Candida isolates at Tepecik Education and Research Hospital. Materials and Methods: Yeast like fungi were isolated between 13.01.2010 and 19.08.2011 at Mycology Laboratory. The identification was done by conventional methods and carbohydrate assimilation profile using the ID32C identification system (Biomerieux, France). Results: Yeast like fungi were isolated from 337 clinical specimens. They consisted of urine, blood culture, respiratory specimen and wound. The most isolated yeast strains were C.albicans (38.6%), C.tropicalis (13.9%), C. parapsilosis (28.4%), C.glabrata (7.4%), C.krusei (3.8%). Conclusion: Recently there is an increment in Candida infections. In this study the most common strain was C.albicans and the rate C. glabrata and C. krusei isolates were lower than expected. C. parapsilosis was the most isolated strain in blood cultures and this may be due to invasive procedures and the use of indwelling catheters.

Clinical and microbiological evaluation of eight patients with isolated Achromobacter xylosoxidans.

Abstract Achromobacter xylosoxidans causes infections in patients with underlying disorders. The aim of this study was to evaluate Achromobacter xylosoxidans cases. Eight patients were included, 5 infections and 3 colonizations. Three of them survived and 2 died. The predisposing factor was catheter. Infection control measures could prevent infection.

Study on seroprevalence of hepatitis delta in a regional hospital in western Turkey

INTRODUCTION Hepatitis delta virus (HDV) is an incomplete virus dependent on hepatitis B virus (HBV) for its multiplication. It can infect individuals with active HBV infection and cause severe liver disease. It is less prevalent than hepatitis B virus, but it causes more serious clinical pictures. In this study we investigated anti-HDV seroprevalance and epidemiological features among HBsAg seropositive outclinic patients at Izmir Tepecik Educational and Research Hospital. METHODOLOGY Serum samples collected from outpatients at Izmir Tepecik Educational and Research Hospital between 1 September 2007 and 30 August 2009 were evaluated. Anti-HDV assay was performed by enzyme immunoassay (EIA). Patients over the age of fourteen who were referred to our hospital were taken into the study. RESULTS Out of 3,094 HBsAg positive patients, 79 (2.5%) had anti-HDV IgG seroprevalance. Of these 79 patients, 42 were hepatitis B carriers, 34 had chronic hepatitis B, two had liver cirrhosis, and one had hepatocellular carcinoma. CONCLUSION Although superinfection and co-infection of HDV are less prevalent than hepatitis B infection, the prognosis is worse as the response to therapy is poor; therefore, patients with hepatitis B should be evaluated further for HDV infection.

The Evaluation of the Distribution and Antimcrobial Susceptibility Profile of the Strains Isolated at Anesthesiology Intensive Care Unit

1 Gülfem Ece1, Tayfun Adanır2 1Tıbbi Mikrobiyoloji Anabilim Dalı, 2Anesteziyoloji ve Reanimasyon Anabilim Dalı, İzmir Üniversitesi, Tıp Fakültesi, Medicalpark Hastanesi, İzmir, Türkiye Yoğun Bakım Suşlarının Dağılım ve Duyarlılığı / Distribution and Susceptibility of Icu Isolates The Evaluation of the Distribution and Antimcrobial Susceptibility Profile of the Strains Isolated at Anesthesiology Intensive Care Unit

The clinical and microbiological evaluation of Sphingomonas paucimobilis strains isolated at our hospital

Objective: Sphingomonas paucimobilis is a Gram negative bacil that is aerobic, nonfermentative yellow pigment forming and has no spore formation. It is prevalent in nature and hospital settings and it is reported that severe infections due to contaminated distilled water, hemodialysis fluid and sterile drug solutions can take place. The aim of our study was to evaluate the clinical and microbiological aspect of the S. paucimobilis strains isolated at various departments of our hospital. Methods: Eleven S. paucimobilis strains isolated from respiratory system, urine, wound and blood specimens at various medical departments of our hospital were evaluated in clinical and microbiological aspect. The identification and antimicrobial susceptibility of the isolates were studied by automatized Vitek version 2.0 (Biomerieux, France). Results: S. paucimobilis was isolated from seven tracheal secretion samples, one wound, one blood culture, and two urine samples. Five isolates were from Anesthesiology and Reanimation Unit, two from Chest Diseases, one from Hematology, one from Dermatology, one from Emergency Department and one isolate was from Pediatric Surgery Department. Two patients were male and nine (81.8%) were female. The age ranged between 1-83(mean 42). Ten of the isolates were resistant to sulbactam/cefaperazone, nine to ciprofloxacin, seven to cefepime, six to ceftazidime, meropenem, and tazobactam/piperacillin, four to amikacine and gentamycine. The demographic data of the patients revealed comorbidities and hospitalization at intensive care unit were present. ÖZET Amaç: Sphingomonas paucimobilis aerop, nonfermentatif, sarı pigment oluşturan, sporsuz, Gram negatif basildir. Doğada ve hastane ortamında yaygın olarak bulunan S. paucimobilis’in distile sular, hemodiyaliz sıvıları, steril ilaç solusyonlarının kontaminasyonuyla ciddi enfeksiyonlara neden olduğu gösterilmiştir. Çalışmamızda hastanemizin çeşitli birimlerinden izole edilen S. paucimobilis suşlarının klinik ve mikrobiyolojik açıdan değerlendirilmesi amaçlandı. Yöntem: Hastanemizin çeşitli birimlerinde yatarak tedavi gören hastaların solunum yolu, idrar, yara ve kan örneklerinden soyutlanan 11 S. paucimobilis izolatı klinik ve mikrobiyolojik açıdan incelendi. İzolatların identifikasyonu ve antibiyotik duyarlılıkları otomatize Vitek 2.0 sistemi (Biomerieux, Fransa) ile yapıldı. Bulgular: Çalışmaya alınan hastaların yedisinin trakeal sekresyonundan, ikisinin idrar birinin yara, birinin kan, örneğinden S. paucimobilis üredi. Suşların beşi Anestezi Yoğun Bakım, ikisi Göğüs Hastalıkları, biri Dermatoloji, biri Hematoloji, biri Çocuk Cerrahisi ve biri de Acil Tıp biriminden izole edildi. Hastaların ikisi erkek ve dokuzu kadın olup yaşları 1-83 arasında değişmekteydi (ort.42). İzolatların 10’u sulbaktam/sefaperazona, dokuzu siprofloksasiline, yedisi sefepime, altısı seftazidim, meropenem, imipenem ve tazobaktam/piperasiline, dördü amikasin ve gentamisine dirençli bulundu. Sonuç: S. paucimobilis ile meydana gelen enfeksiyonların genellikle immunsuprese hasta grubunda meydana geldiği gösterilmiştir. Çalışmamızda S. paucimobilis üremesi olan hastaların büyük bölümünün Cilt 70  Sayı 4  2013 Turk Hij Den Biyol Derg 182 İlk kez 1990 yılında Yabuuchi ve ark. tarafından tanımlanan Sphingomonas paucimobilis, aerop, nonfermentatif, sarı pigmentli, spor oluşturmayan, Gram negatif bir basildir. Doğada ve hastane ortamında yaygın olarak bulunur. Nadiren hayatı tehdit eden enfeksiyonlara yol açmaktadır. S. paucimobilis, daha önce Pseudomonas grubunda sınıflandırılırken günümüzde 30’dan fazla tür Sphingomonas cinsi içinde sınıflandırılmaktadır (1). Sphingomonas’ın, distile sular, hemodiyaliz sıvıları ve steril ilaç solüsyonlarıyla bulaş gösterdiği ve özellikle immunsupresif hastalarda salgınlara yol açtığı gösterilmiştir (2). S. paucimobilis, bakteriyemi, septik artrit, pnömoni, postoperatif endoftalmit, kateter ile ilişkili enfeksiyonlar gibi birçok enfeksiyona ve kontamine olmuş hastane ekipmanı veya tıbbi araçların manipulasyonu ile de fırsatçı hastane enfeksiyonlarına neden olabilir (3). Çalışmamızda hastanemizin çeşitli birimlerinden izole edilen S. paucimobilis suşlarının klinik ve mikrobiyolojik açıdan değerlendirilmesi amaçlandı.