nan Gunanti

Subjective and objective observation of skin graft recovery on Indonesian local cat with different periods of transplantation time

Aim: The success of a skin graft in a cat is highly dependent on the granulation formed by the base of recipient bed. Granulation by the base of recipient bed will form after several days after injury. This research aimed to observe subjective and objective profile of skin graft recovery on forelimb of cats with different periods of donor skin placement. Materials and Methods: Nine male Indonesian local cats aged 1-2 years old, weighing 3-4 kg were divided into three groups. The first surgery for creating defect wound of 2 cm×2 cm in size was performed in the whole group. The wound was left for several days with the respective interval for each group, respectively: Group I (for 2 days), Group II (for 4 days), and Group III (for 6 days). In the whole group, the second surgery was done by the harvesting skin of thoracic area which then applied on recipient bed of respective groups. Result: The donor skin on Group II was accepted faster compared to Group I and Group III. The donor skin did not show color differences compared to surrounding skin, painless, bright red in bleeding test had faster both hair growth and drug absorption. Test toward the size of donor skin and the effect of drugs did not show a significant difference between each group. Conclusion: The observe subjective and objective profile of skin graft recovery on forelimb of cats on Group II were accepted faster compared to Group I and III.

Changes in histopathology and cytokeratin AE1/AE3 expression in skin graft with different time on Indonesian local cats

Aim: A good skin graft histopathology is followed by formation of hair follicle, sweat gland, sebaceous gland, blood vessel, lightly dense connective tissue, epidermis, and dermis layer. This research aimed to observe histopathology feature and cytokeratin AE1/AE3 expression on cat skin post skin grafting within a different period of time. Materials and Methods: Nine male Indonesian local cats aged 1-2 years old weighing 3-4 kg were separated into three groups. First surgery created defect wound of 2 cm × 2 cm in size to whole groups. The wounds were left alone for several days, differing in interval between each group, respectively: Group I (for 2 days), Group II (for 4 days), and Group III (for 6 days). The second surgery was done to each group which harvested skin of thoracic area and applied it on recipient wound bed. On day 24th post skin graft was an examination of histopathology and cytokeratin AE1/AE3 immunohistochemistry. Results: Group I donor skin’s epidermis layer had not formed completely whereas epidermis of donor skin of Groups II and III had completely formed. In all group hair follicle, sweat gland, sebaceous gland, and neovascularization were found. The density of connective tissue in Group I was very solid than other groups. Cytokeratin AE1/AE3 expression was found on donor skin’s epithelial cell in epidermis and dermis layer with very brown intensity for Group II, brown intensity for Group II, and lightly brown for Group I. Conclusion: Histopathological structure and cytokeratin AE1/AE3 expression post skin graft are better in Groups II and III compared to Group I.

Assessment of prolonged tissue response to porousiron implant by radiodensity approach

Porous iron has been recently introduced as new biomaterials for bone scaffolds. As a metal that degrades in the in vivo setting (biodegradable metal), little has been known for its cell-material interaction within the living tissue especially in the view of its applications for bone implant materials. Therefore, the aim of this study is to assess tissue response to the implantation of porous iron in the in vivo setting using rats as animal model and radiographic examination. Ten adult Sprague Dawley rats received the implantation of porous iron implants having different porosity into their femoral bone followed by radiographic examination up to 5 months post-implantation. Results shows differences in the opacity of radiographic images where the implants looked more radiopaque than bone and muscle. Radiodensity values of the implants decreases overtime indicating they experienced progressive degradation. This values dynamically change over time up to 5 months in relation to the event of chronic inflammation, degradation and bone healing process.