Gunther Hartmann

Manual of antisense methodology

Preface G. Hartmann, S. Endres. Contributors. Part I: The chemistry of antisense oligonucleotides. 1. Chemical synthesis and purification of phosphorothioate antisense oligonucleotides Y.S. Sanghvi, et al. 2. How to choose optimal antisense targets in an mRNA B. Schu, H. Brinkmeier. Part II: Antisense application in vitro. 3. How to characterize and improve oligonucleotide uptake into leukocytes M. Bidlingmaier, et al. 4. Strategies for targeted uptake of antisense oligonucleotides in hepatocytes W.-B. Offensperger, et al. 5. How to exclude immunostimmulatory and other nonantisense effects of antisense oligonucleotides A.M. Krieg. 6. Testing antisense oligonucleotides in controlled cell culture assays M. Flanagan. 7. Inhibition of TNF synthesis by antisense oligonucleotides M. Taylor, L. Kobzik. 8. Inhibition of IGF-1 receptor expression by antisense oligonucleotides in vitro and in vivo M. Resnicoff. Part III: Antisense application in vivo. 9. How to test antisense oligonucleotides in animals R. Spanagel, et al. 10. Lipid-based carriers for the systemic delivery of antisense drugs M. Webb. 11. Designing a clinical antisense study F.E. Cotter, D. Fennell. Index.