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Dive into the research topics where Guo Meijin is active.

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Featured researches published by Guo Meijin.


Frontiers of Biology in China | 2006

High cell density and high expression of recombinant human ApoA-lMilano in Escherichia coli by twice temperature-shifted induction

Zhuang Yingping; Ma Wenfeng; Ding Mansheng; Guo Meijin; Chu Ju

The effect of temperature on the formation of recombinant protein, apolipoprotein A-IMilano was investigated in the present study. The temperature of the initial growth phase was set at 30°C, while temperature variation in induction phase was arranged in three modes. High cell-density culture of Escherichia coli and high expression of recombinant human by twice temperature-shifted induction were carried out. Experimental results showed that ApoA-IMilano reached 4.8 g/L with the final cell density of OD600, 150. It was found that twice temperature-shifted induction could successfully avoid the effect of acetic acid on cell density and the expression of the product. The present study provides a basic procedure for the production of recombinant ApoA-IMilano.


Archive | 2001

Full-synthetic culture medium and its application

Zhang Siliang; Guo Meijin; Chu Ju


Archive | 2015

Method for increasing oxytetracycline yield of streptomyces rimosus

Guo Meijin; Zheng Zijing; Yao Gaofeng; Chu Ju; Zhuang Yingping; Zhang Siliang; Qian Jiangchao


Archive | 2015

Method used for detecting n-propyl alcohol in erythromycin fermentation processes using electronic olfactory detector

Guo Meijin; Zhao Hongtu; Sun Jingchun; Zhang Rongkai; Wang Zejian; Chu Ju; Zhuang Yingping; Zhang Siliang


Archive | 2013

On-line cell microscopic observer for biological reactor

Miao Shouhong; Wang Zejian; Zhang Min; Yu Wan An; Guo Meijin; Wang Zhenyu; He Liqing; Zhang Dawei; Yuan Xujun


Archive | 2004

Method for preparing phytase by the fermentation of methanol nutrient type recombinant yeast

Zhang Siliang; Guo Meijin; Chu Ju


Archive | 2014

Online cell microscopic observation instrument used in bioreactor

Miao Shouhong; Wang Zejian; Zhang Min; Yu Wan An; Guo Meijin; Wang Zhenyu; He Liqing; Zhang Dawei; Yuan Xujun


Archive | 2013

Method for amplifying mesenchymal stem cells on large scale

Zhou Lang; Guo Meijin; Zhang Siliang; Chu Ju; Zhuang Yingping


Archive | 2013

Novel process for fermenting coenzyme Q10 based on online oxygen consumption rate control

Chen Zhaofeng; Hu Weijiang; Li Yong; Chen Xiaobing; Lv Yong; Lv Jialiang; Li Weifeng; Yang Xiaofeng; Wang Zejian; Guo Meijin


Archive | 2017

Method of increasing suspendible cultured embryonic callus induction ratio of momordica grosvenori

Song Yunfei; Guo Meijin; Li Jiarui; Wang Zejian; Zhuang Yingping; Li Yuyu

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Zhuang Yingping

East China University of Science and Technology

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Chu Ju

East China University of Science and Technology

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Zhang Siliang

East China University of Science and Technology

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He Liqing

Shanghai University of Science and Technology

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Ma Wenfeng

East China University of Science and Technology

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Yuan Xujun

Shanghai University of Science and Technology

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Wang Zhenyu

Capital Medical University

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Ding Mansheng

East China University of Science and Technology

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Zhang Xiaolin

South China University of Technology

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