Guoxin Song

Determination of the volatile constituents of ChineseCoriandrum sativum L. by gas chromatography—Mass spectrometry with solid-phase microextraction

SummaryFifteen main volatile compounds in ChineseCoriandrum sativum L. were separated and identified by gas chromatography—mass spectrometry (GC-MS) combined with solid-phase microextraction (SPME). Fresh ChineseCoriandrum sativum L. was ground and its volatile compounds were extracted by SPME with a 100 μm polydimethylsiloxane fiber. The fibers were desorbed in a GC injection liner at 250°C for 3 min. More than 15 peaks were separated by headspace SPME-GC-MS analysis. The main compounds in headspace ofCoriandrum sativum L. identified by mass spectrometry included decanal, 2-decenal, 1-decanol,trans-2-decen-1-ol,trans-2-decen-1-al,trans-2-tridecenal etc, which were verified by reference compounds. Their relative contents were calculated on basis of peak areas. SPME extraction conditions and capillary chromatography column used to separate the volatile compounds were investigated.

Use of solid-phase microextraction as a sampling technique for the characterization of volatile compounds emitted from Chinese daffodil flowers

Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) has been applied to the determination of volatile compounds emitted from living daffodil flowers. The SPME conditions were optimized and applied to headspace extraction of the volatile compounds. The volatile compounds adsorbed on the fiber were desorbed and analyzed by GC-MS. We identified 27 compounds in the flower emission, which mainly included acetic acid phenethyl ester (31.68%), E-ocimene (17.15%), acetic acid benzyl ester (11.53%), neo-allo-ocimene (6.94%), allo-ocimene(5.34%), α-linalool (5.26%), 1,8-cineole(3.70%), benzenepropyl acetate (1.98%), and 3-methyl-2-buten-1-ol acetate(1.88%). The volatile compounds emitted by the flower excised from the daffodil were also analyzed by the present method, and the results showed that n-pentadecane, n-hexadecane, n-octadecane, and acetic acid phenethyl ester might be biomarker compounds of living daffodil flowers. To our knowledge, this is the first report on the volatile compounds emitted from Chinese daffodil flowers.