Gustavo Hitzschky Fernandes Vieira

In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts.

OBJECTIVE To evaluate the antibacterial effect of aqueous and ethanolic moringa leaf extracts (Moringa oleifera) on the growth of gram-positive and negative bacteria. METHODS Paper disks were soaked with 100, 200, 300 and 400 μL of extract at 20 g/180 mL and 10 g/190 mL. All extracts were tested against Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923), Vibrio parahaemolyticus, Enterococcus faecalis (ATCC29212), Pseudomonas aeruginosa (ATCC27853), Salmonella enteritidis (IH) and Aeromonas caviae. The susceptibility tests were performed using the modified disk diffusion method. RESULTS The strains E. coli, P. aeruginosa and S. enteritidis (IH) were resistant to all treatments. In general, disks with 400 μL extract were the most efficient against S. aureus, V. parahaemolyticus, E. faecalis and A. caviae. CONCLUSIONS The study indicates a promising potential for aqueous and ethanolic Moringa leaf extracts as alternative treatment of infections caused by the tested strains.

Susceptibilidade "in vitro" a antimicrobianos de estirpes de Vibrio spp isoladas de camarões (Litopenaeus vannamei) e de água de criação destes animais provenientes de uma fazenda de camarões no Ceará Nota prévia

Foram feitos ensaios de susceptibilidade a antibioticos em 48 cepas de Vibrio isoladas do cultivo do Litopenaeus vannamei no Ceara. Para os testes de difusao foram utilizados 11 antibioticos. A especie que apresentou maior percentagem de resistencia aos antimicrobianos foi V. cholerae, onde 33,33% das 12 cepas testadas mostraram-se resistentes a sulfazotrim, 25% a ampicilina e 33,33% a ceftriaxona.

Bactérias entéricas presentes em amostras de água e camarão marinho litopenaeus vannamei oriundos de fazendas de cultivo no estado do Ceará, Brasil

Foram realizados a quantificacao de coliformes totais (CT) e termotolerantes (CTT), isolamento e identificacao de coliformes, e pesquisa de Salmonella em 28 amostras de agua e 28 de camarao da especie Litopenaeus vannamei, oriundas de duas fazendas de cultivo localizadas no Estado do Ceara, Brasil. Nenhuma amostra de agua apresentou indice de CTT acima do limite de 2.500/100 mL preconizado pela legislacao para aguas salobras destinadas ao cultivo de organismos para fins de consumo. O Numero Mais Provavel (NMP/g) de CTT das amostras de camarao variou de <3 a 2,9 x 10(4). A maior frequencia de isolamento de coliformes nas amostras de agua e camarao foi a da especie Escherichia coli. Em apenas tres (5,35%), das 56 amostras analisadas, foi detectada a presenca de Salmonella sorovar Newport e S. Saintpaul. Apesar do baixo indice de CTT e da baixa incidencia de salmonela, a presenca dessas bacterias entericas em ambientes de cultivo de peneideos e preocupante, uma vez que podem provocar infeccoes em humanos.

Quantification and distribution of vibrio species in water from an estuary in Ceará-Brazil impacted by shrimp farming

. The pH value was the environmental factor most strongly associated with the abundance of vibrios. Sixty-two vibrio strains were isolated belonging to 15 species (6 of which observed in June-September and 8 in October). The most frequently isolated species were V. parahaemolyticus and V. cholerae .

Production of protein hydrolysate from Lobster (Panulirus spp.)

Abstract Protein hydrolysates were prepared using waste meat from lobsters ( Panulirus spp.) from the northeast coast of Brazil. Solubilization of the protein, measured by the liberation of tyrosine, was conducted with proteolytic enzymes. A statistical analysis of the hydrolysis proces results indicated that they were dependent on time, type of enzyme, and the enzyme concentration. The optimum results, including those of total amino acids and free amino acids analyses, were obtained for the products prepared using a fungal protease. A statistical analysis of the results of a taste panel was conducted and the potential of employing these hydrolysates as flavourants is presented. The functional properties of the products were reported as good.