Gyu Man Kim

Estimation of cutter deflection and form error in ball-end milling processes

This paper presents a method to analyze the 3-dimensional form error of a ball-end milled surface due to the elastic compliance of the cutting tool. In order to estimate the form error in various cutting modes, the cutting force and the cutter deflection models including the effect of the surface inclination were established. The cutting forces were calculated by using the cutter contact area determined from the Z-map of the surface geometry and the current cutter location. The tool deflection responding to the cutting force was then calculated by considering the cutter and the holder stiffness. The cutter was modeled as a cantilever beam consisting of the shank and the flute. The stiffness of the holder was measured experimentally. Various experimental works have been performed to verify the validity of the proposed model. It is shown that the proposed method is capable of accurate prediction of cutting forces and the surface form error.

Microfluidic formation of pH responsive 5CB droplets decorated with PAA-b-LCP

We are reporting for the first time the pH responsiveness of liquid crystal (LC) microdroplets decorated with an amphiphilic block copolymer of PAA-b-LCP. We successfully demonstrated the adsorption of block copolymer on LC droplets by fluorescence microscopy and pH response to the radial-to-bipolar orientational change of the LC droplets by changing pH from 12 to 2 through the polarized optical microscope (POM). We believe that our results may pave the way for the generation of monodisperse droplets decorated by various amphiphilic block copolymers which respond to several kinds of the external stimuli. These developments may be important for potential applications of the LC droplets in sensing and encapsulation fields.

Surface modification with self-assembled monolayers for nanoscale replication of photoplastic MEMS

A release technique that enables to lift microfabricated structures mechanically off the surface without using wet chemistry is presented. A self-assembled monolayer of dodecyl-trichlorosilane forms a very uniform /spl sim/1.5-nm-thick anti-adhesion coating on the silicon dioxide surface, on full wafer scale. The structural layers are formed directly onto the organic layer. They consist here of a 100-nm-thick aluminum film and a high-aspect ratio photoplastic SU-8 structure. After the microfabrication the structure can be lifted off the surface together with the aluminum layer. This generic technique was used to make a variety of novel structures. First, aluminum electrodes that are embedded in plastic are made using lithography, etching and surface transfer techniques. Second, using a patterned monolayer as defined by microcontact printing, resulted in a spatial variation of the surface adhesion forces. This was used to directly transfer the stamped pattern into a metal structure without using additional transfer etching steps. Third, the monolayers ability to cover surface features down to nanometer scale was exploited to replicate sharp surface molds into metal coated photoplastic tips with /spl sim/30-nm radii for use in scanning probe instruments such as near-field optical techniques. The advantage compared to standard sacrificial layer techniques is the ability of replication at the nanoscale and the absence of etchants or solvents in the final process steps.

A Hemodynamic Study on a Marginal Cell Depletion Layer of Blood Flow Inside a Microchannel

Biological flows, especially blood flow, have attracted a great deal of attention from fluid engineering and hemodynamic investigation fields with advances in bio-technology. The flow of blood carries dissolved gases, nutrients, hormones, and metabolic waste through the circulatory system in the human body. In the present study, the characteristics of blood flow inside a microchannel are investigated by using a micro-particle image velocimetry (micro-PIV) and an optical image processing technique. The motion of red blood cells (RBCs) was visualized with a high-speed CCD camera. The microchannel is made of polydimethylsiloxane (PDMS) material and a slide-glass is attached to the top. The thickness of the margin cell depletion layer is calculated from an acquired raw image through the image processing method, with variations in microchannel width.

Fabrication of HepG2 Cell Laden Collagen Microspheres using Inkjet Printing

In this study, drop-on-demand system using piezo-elecrtric inkjet printers was employed for preparation of collagen microspheres, and its application was made to the HepG2 cell-laden microsphere preparation. The collagen microspheres were injected into beaker filled with mineral oil and incubated in a water bath at 37℃ for 45 minutes to induce gelation of the collagen microsphere. The size of collagen microsphere was 100μm in diameter and 80μm in height showing spherical shape. HepG2 cells were encapsulated in the collagen microsphere. The cell-laden microspheres were inspected by the microscopic images. The encapsulation of cells may be beneficial for applications ranging from tissue engineering to cell-based diagnostic assays.

EXPERIMENTAL STUDY ON BASIC PERFORMANCE OF ELECTROOSMOTIC PUMP WITH ION EXCHANGING POROUS GLASS SLIT

The basic concept and preliminary performance results of a miniaturized electroosmotic pump with diaphragms were included in the present study. The separation of an electroosmotic pumping liquid from a drug using diaphragms is mainly to have a freedom in choosing an electroosmotic pumping liquid and to achieve the optimal drug delivery with its preferable precise control. As a result, the maximum flow rate and current increased linearly according to the increment of applied voltage that is electric potential.

THE FEASIBILITY OF FLUORESCENT NANO-PARTICLE FOR BIOLOGICAL FLOW ANALYSIS IN A MICROCHANNEL

The biological flow characteristics inside a microchannel were investigated experimentally using a micro-particle image velocimetry (micro-PIV) method. The main objectives of this study were to understand the blood flow in micro-domain blood vessels and to identify the feasibility of nano-scale fluorescent particles for velocity measurement. The flow field was analyzed with a spatial resolution of 1K×1K pixels at low Reynolds number flow. To obtain the spatial distributions of mean velocity, 100 instantaneous velocity fields were captured and ensemble-averaged. As a result, for the case of blood flow, there were substantial velocity variations in the central region of micro-channel due to the presence of blood cells in the blood flow.

Porous polymer coatings on metal microneedles for enhanced drug delivery

We present a simple method to coat microneedles (MNs) uniformly with a porous polymer (PLGA) that can deliver drugs at high rates. Stainless steel (SS) MNs of high mechanical strength were coated with a thin porous polymer layer to enhance their delivery rates. Additionally, to improve the interfacial adhesion between the polymer and MNs, the MN surface was modified by plasma treatment followed by dip coating with polyethyleneimine, a polymer with repeating amine units. The average failure load (the minimum force sufficient for detaching the polymer layer from the surface of SS) recorded for the modified surface coating was 25 N, whereas it was 2.2 N for the non-modified surface. Calcein dye was successfully delivered into porcine skin to a depth of 750 µm by the porous polymer-coated MNs, demonstrating that the developed MNs can pierce skin easily without deformation of MNs; additional skin penetration tests confirmed this finding. For visual comparison, rhodamine B dye was delivered using porous-coated and non-coated MNs in gelatin gel which showed that delivery with porous-coated MNs penetrate deeper when compared with non-coated MNs. Finally, lidocaine and rhodamine B dye were delivered in phosphate-buffered saline (PBS) medium by porous polymer-coated and non-coated MNs. For rhodamine B, drug delivery with the porous-coated MNs was five times higher than that with the non-coated MNs, whereas 25 times more lidocaine was delivered by the porous-coated MNs compared with the non-coated MNs.

Neuropeptide Y improves cisplatin-induced bone marrow dysfunction without blocking chemotherapeutic efficacy in a cancer mouse model

Cisplatin is the most effective and widely used chemo-therapeutic agent for many types of cancer. Unfortunately, its clinical use is limited by its adverse effects, notably bone marrow suppression leading to abnormal hematopoiesis. We previously revealed that neuropeptide Y (NPY) is responsible for the maintenance of hematopoietic stem cell (HSC) function by protecting the sympathetic nervous system (SNS) fibers survival from chemotherapy-induced bone marrow impairment. Here, we show the NPY-mediated protective effect against bone marrow dysfunction due to cisplatin in an ovarian cancer mouse model. During chemotherapy, NPY mitigates reduction in HSC abundance and destruction of SNS fibers in the bone marrow without blocking the anticancer efficacy of cisplatin, and it results in the restoration of blood cells and amelioration of sensory neuropathy. Therefore, these results suggest that NPY can be used as a potentially effective agent to improve bone marrow dysfunction during cisplatin-based cancer therapy.

PDMS membrane based force sensor: Basic structure design and assessment

Haptic is a tactile feedback technology which has the advantage of the sense of touch by applying forces, vibrations or motions to the user. To use the haptic device for remote control, it is need to sense the object. These sensors are called as haptic sensor, and it senses the magnitude, direction, and distribution of a force. The sensed force data are used to reproduce the boundary limit of remote users movement or the reactions from objects. We develop an elastic material based tactile sensor for sensing the reaction force and the shape of contact surface. Sensor surface is made of Poly-dimethylsiloxane(PDMS) and it has small patterns with chrome. The deformation of these patterns is calculated by computer vision. By this process we could know the amount force that gave from some object. We performed the experiment with force sensor to find the relationship between the force and deformation of surface.