Gyula Mikite

Interaction of amino acids and glutathione with the fungicide 1-phenyl-2-nitro-3-acetoxyprop-1-ene studied by charge-transfer chromatography

Abstract Reversed-phase charge-transfer thin-layer chromatography has been applied to study the interaction of the fungicide 1-phenyl-2-nitro-3-acetoxyprop-1-ene with amino acids and glutathione. The fungicide interacts preferentially with thiol amino acids and glutathione, but weak interactions with other amino acids were also observed. Our results confirm that the fungicide can act by blocking the sulfhydryl groups of essential proteins in fungi. The interaction with thiol amino acids involves molecular complex formation, but that with glutathione is of covalent character. The pH value has no significant effect on the interaction.

Complexation of nitrostyrenes with soluble β-cyclodextrin polymer studied by reversed-phase thin-layer chromatography

Cyclodextrin complexation decreases the apparent lipophilicity of hydrophobic guest molecules. A higher complex stability results in a larger decrease of lipophilicity as determined by reversed-phase thin-layer chromatography. The method was applied to study the complex formation of 33 nitrostyrene derivatives with a water soluble cross linked β-cyclodextrin polymer (weight average molecular weight: 4300). The substituents in thepara position of the benzene ring had a higher impact on the complex stability than those in themeta andortho positions. The substituents on the alkyl side chain influenced the complex stability to the same extent as those on the benzen ring.