H.J.A. Fleury

An in Vitro Study of the Susceptibility of Cells from a Meningioma of Human Origin for Measles Virus

A cell culture from a meningioma of human origin was found permissive for the Edmonston strain of measles virus. Virus was isolated from the supernatant of infected cell sheet as soon as 48 hr post-inoculation. Cytopathic effect, observed by light microscopy, exhibited syncitia plus intracytoplasmic and intranuclear inclusions. Electron microscopy enabled viral nucleocapsids to be observed first in the cytoplasm and secondly within the nucleus of infected cells.

Isolation of an adeno-like virus from two cases of strawberry disease in rainbow trout

Summary An adeno-like virus was isolated from skin lesions of two cases of strawberry disease in rainbow trout. This is the first reported observation of an adeno-like virus in fish. If the etiological link with strawberry disease is confirmed, this virus could be referred to as trout strawberry disease virus or TSDV.

Replication of measles virus in a cell culture from a glioblastoma of human origin.

A cell culture from a glioblastoma of human origin infected with the Edmonston strain of measles virus produced and released infectious measles virus. All cellular types seemed to be involved in the process of virus replication. Staining with hematin-eosin revealed the presence of eosinophilic intracytoplasmic and intranuclear inclusions. Examination with the electron microscope revealed viral nucleocapside in the cytoplasm and, rarely, in the nuclei of infected cells.

Susceptibility of the guinea-pig to Paramyxovirus Yucaipa

Summary When inoculated by the intranasal route with Paramyxovirus Yucaipa, the guinea-pig (Cavia porcellus) (1) produced infectious virus in the nasal specimens, (2) exhibited tracheitis, as well as bronchitis and pneumonia, as shown by anatomopathological observation, and (3) elaborated virusspecific antibodies. We thus concluded that the guinea-pig is susceptible to Paramyxovirus Yucaipa.

Isolation of paramyxoviruses of the PMV-1 serotype from pigeons captured in the city of Bordeaux

Summary Seven paramyxoviruses of the PMV-1 serotype (NDV) were isolated from the faeces of 350 pigeons captured in the city of Bordeaux. These infected pigeons may represent a source of contamination for humans living in the vicinity.

Intracellular Recordings from Prolactin-Secreting Pituitary Cells in Culture: Evidence for a Direct Action of Estrogen on the Cell Membrane

The effects of sex steroids on the central nervous system are commonly explained according to the general scheme of steroid hormone action involving the nucleus following binding to a cytoplasmic receptor and translocation to the nucleus. However, since estrogen acts on brain and pituitary with latencies ranging from seconds and minutes to hours and days, it is unlikely that a single mechanism of action is involved. At the hypothalamic level, the short latency effect of sex steroid has been reported on the electrical activity of hypothalamic neurons (Kelly et al., 1976; Dufy et al., 1976), which is hardly compatible with the latencies required by genomic activation. Moreover, action potentials are not only a property of neurons, but also are detected in endocrine cells including normal (Taraskevich and Douglas, 1977) and clonal pituitary cells (Kidokoro, 1975), where they have been related to the secretory process.

A comparison of the fatty acids of four measles and SSPE viruses

Summary Edmonston and Schwarz vaccine measles viruses and Mantooth and Halle SSPE viruses were grown in Vero cells, purified, and used for a biochemical investigation of their fatty acids. Results showed that: u - measles virus contained all types of fatty acids existing in the cell (C 12 , C 14 , C 16 , C 16:1 , C 18 , C 18:1 ); - the four measles and SSPE viruses in the experiment exhibited the same qualitative composition in their fatty acids, but slightly different quantitative composition; - these quantitative compositions do not represent a useful marker for unequivocal differentiation of SSPE viruses from measles virus

Differences in Early Ultrastructural Aspects of the Replication of Measles and Subacute Sclerosing Panencephalitis Viruses in a Cell Culture from a Human Astrocytoma

Monolayer cultures from a human astrocytoma were infected with small amounts of Mantooth Subacute Sclerosing Panencephalitis (SSPE) and Edmonston measles viruses. The infected cells were studied with an electron microscope 48 hours and 96 hours post-inoculation (PI). By 48 hours PI, both viruses produced syncytia and cytoplasmic inclusions of granular nucleocapsids 20 to 25 nm in diameter which did not differ in appearance. With the Edmonston measles virus granular nucleocapsids assembled into budding particles were found just under the cell membrane while nucleocapsids of Mantooth SSPE virus spared the area under the cell membrane and were not incorporated into budding particles. Inclusions of smooth nucleocapsids, 15 nm in diameter, could be seen within the nuclei of Mantooth SSPE virus infected cells 96 hours PI; such nuclear inclusions were not found in the Edmonston measles virus infected cells. These results are compared with those obtained in other cell systems and are discussed with respect to recent findings in the field of SSPE.

Biochemical characterization of a Yucaipa-like virus.

A Yucaipa-like virus (PLOC/Senegal/273/77) was grown in embryonated chicken eggs and used for biochemical investigations after purification. The genome of the virus is composed of one fragment of single-stranded (ss)RNA with an estimated mol. wt. of 5.6 X 10(6). There are six virus structural polypeptides with mol. wt. of 126 000, 68 000 (major), 60 000, 52 000 (major), 44 000 and 39 000 (major). The fatty acid composition of the virus envelope seems to be very selective since we found only fatty acids containing 14 and 16 carbon atoms.

Infection du cerveau de souris par le Bunyavirus Germiston: Donnés morphologiques et moléculaires

Summary Groups of 9–15-day old Swiss mice were inoculated intracerebrally with the Bunyavirus Germiston and brains were studied by morphologic and molecular techniques. With the onset of neurologic signs, lesions were mainly found in periventricular regions and in the hippocampus; viral particles 90 to 95 nm in diameter could be observed within the cytoplasm of infected cells. In situ hybridization with a DNA probe complementary to the S segment of the viral genome was consistent with the morphologic data.