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Dive into the research topics where H. Wahid is active.

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Featured researches published by H. Wahid.


Animal Reproduction Science | 2010

Effect of sugars on characteristics of Boer goat semen after cryopreservation.

S.W. Naing; H. Wahid; K. Mohd Azam; Y. Rosnina; A.B. Zuki; S. Kazhal; M.M. Bukar; Myint Thein; T. Kyaw; Maung Maung San

In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucrose and trehalose, glucose and trehalose), and control (glucose without trehalose) (c) different concentrations of trehalose on cryopreservation using Tris based extender. The total motility, forward motility, viability, normal spermatozoa, acrosome integrity and membrane integrity were assessed subjectively. Differences were not detected among monosaccharides, but glucose increased (P<0.05) sperm forward motility in post-thaw goat semen compared to trehalose or sucrose supplementation. Semen quality did not differ (P>0.05) among disaccharide sugar supplementation. Combination of glucose and trehalose significantly improved the characteristics of Boer spermatozoa after cryopreservation (P<0.05). Supplementation of trehalose (198.24mM) into the glucose extender significantly increased total motility, forward motility, live spermatozoa, acrosome integrity and membrane integrity following cryopreservation (P<0.05). In conclusion, glucose had the better ability to support Boer sperm motility and movement patterns. Combination of monosaccharide (glucose) and disaccharide (trehalose) improved semen quality following cryopreservation. Trehalose supplementation at the concentration of 198.24mM to the glucose extender conferred the greater improvement of semen quality for Boer semen cryopreservation.


Anatomia Histologia Embryologia | 2002

Gross Anatomy and Ultrasonographic Images of the Reproductive System of the Sumatran Rhinoceros (Dicerorhinus sumatrensis)

Z. Zainal Zahari; Y. Rosnina; H. Wahid; M.R. Jainudeen

The Sumatran rhinoceros (Dicerorhinus sumatrensis) is the smallest of all the rhino species. It is one of the rarest mammals in the world and is in imminent danger of extinction. A study was carried out on seven wild‐caught females, three wild‐caught males and one captive born female Sumatran rhinoceros at the Sumatran Rhinoceros Breeding Centre in Sungai Dusun, Selangor, Malaysia, beginning 1990. As a result of the paucity of scientific information on the reproductive biology of the Sumatran rhinoceros, this study was conducted to obtain information, which could assist in the captive breeding of this endangered and near extinct species. The anatomy of the reproductive system was based on two post‐mortem specimens and transrectal real‐time ultrasonography in six adult females. Genitalia of the Sumatran rhinoceros were similar to those of other species of rhinoceroses. The cervix consisted of several folds, the uterus was bicornuate with a short body and prominent horns and the ovaries were completely covered by the fimbriated end of the fallopian tube. The internal genitalia could be imaged by ultrasonography. The testes were located within a pendulous scrotum. Two lateral projections were located at the base of the penis. A well‐defined process glandis was present at the tip of the penis. The accessory sex glands and the testes could be imaged by ultrasonography.


Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2011

Cryotop and development of vitrified immature bovine oocytes

H. Hajarian; H. Wahid; Y Rosnina; M. Daliri; M. Dashtizad; H. Karamishabankareh; O Abas Mazni

The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes using OPS and EMG showed 26 and 32%; and 35 and 46% of PB and MII rates, respectively. The highest survivability resulted from Cryotop and EMG groups and no significant difference was found between them. Vitrified oocytes using Cryotop had the highest cleavage and blastocyst rates. All of the mean rates for vitrified immature oocytes were significantly lower than that of control group (P<0.05). The results of this study showed the superiority of Cryotop device for vitrification of immature bovine oocytes


Reproductive Biomedicine Online | 2010

92 EFFECTS OF EXPOSURE TO DMSO IN VITRIFICATION SOLUTION ON CYTOTOXICITY AND IN VITRO VIABILITY OF IMMATURE BOVINE OOCYTES

H. Hajarian; H. Wahid; O. Abas Mazni; Y. Rosnina; M. Daliri; M. Dashtizad

Based on previous studies for vitrification of oocytes, it has been shown that short term exposure to DMSO during vitrification could improve the maturation rate and cause not spontaneous parthenogenesis (Isachenko et al., 2006). In addition, it was reported that DMSO in freezing media caused disassembly of microfilaments and chromosomal abnormalities in mouse oocytes (Vincent et al., 1990). On the other hand, DMSO is categorized as a potent glass former and its existence in vitrification solution seems necessary. The aim of this study was to determine the in vitro viability of immature bovine oocytes vitrified by short or long time exposure to DMSO. Materials and Methods: Cumulus oocytes complexes (COCs) with homogenous ooplasm were recovered from slaughterhouse ovaries and used in this study. The vitrification protocol was adapted from Kuwayama et al (2005) with minor modifications. Briefly, oocytes were washed twice in holding solution (HS, Hepes-buffered TCM medium supplemented with 20% fetal calf serum, FCS) and kept there for about 15 min. Group of four COCs were incubated in the first vitrification solution (VS1; 7.5% DMSO and 7.5% EG in HS) for 12 min. Equilibration in VS1 was performed in three steps of increasing concentration. First (F) and second (S) steps contained 1/3 and 2/3 of VS1 diluted in HS, and the third (T) step contained only pure VS1. Based on removal of DMSO from each step, five treatment groups were designed: (G1) control, (G2) VS1, (G3) F w/o DMSO, (G4) F+S w/o DMSO, and (G5) F+S+T w/o DMSO. For G3, G4 and G5, similar concentration of EG was added to replace DMSO in VS1. All treatment groups were equilibrated into the second vitrification solution (VS2; 15% DMSO, 15% EG and 0.5M sucrose in HS) for a further 60 sec. Two experiments were performed: (a) cytotoxicity after only exposure, and (b) in vitro viability after vitrification processes. In cytotoxicity test, immature oocytes were directly transferred to the warming solution (WS). In vitrification experiment, oocytes were instantly loaded on a Cryotop device and submerged into liquid nitrogen (LN2) for storage. The time of exposure from VS2 to LN2 was not longer than 90 s. Vitrified samples were maintained in LN2 for at least 10 days. Immediately after removing the Cryotop from LN2, thin strip of Cryotop was submerged in 3 ml HS plus 1M sucrose (WS; 39°C) and smoothly tried to detach oocytes from Cryotop device. Immature oocytes were left in WS for one minute and then transferred to HS plus 0.5M and 0.15M sucrose solution for 3 and 5 min, respectively. Finally, the immature oocytes were washed twice in HS for 5 min each and processed for in vitro maturation. Significant differences among treatments used in the experiment were revealed by one-way analysis of variance and followed by Duncans multiple range test for mean comparisons (P < 0.05) using SAS software (ver. 9.1).


Archive | 2016

Sheep and Goats

M.R. Jainudeen; H. Wahid; E.S.E. Hafez


Archive | 2016

Ovulation Induction, Embryo Production and Transfer

M.R. Jainudeen; H. Wahid; E.S.E. Hafez


Animal Reproduction Science | 2005

Reproductive behaviour of captive Sumatran rhinoceros (Dicerorhinus sumatrensis)

Z. Zainal Zahari; Y. Rosnina; H. Wahid; K. C. Yap; M.R. Jainudeen


Reproduction in Domestic Animals | 2013

Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa.

Kazhal Sarsaifi; Y. Rosnina; Mo Ariff; H. Wahid; Homayoun Hani; Nurhusien Yimer; Jaya Vejayan; S Win Naing; Mo Abas


Tropical Animal Health and Production | 2010

Ovarian activity in beef and dairy cows with prolonged postpartum period and heifers that fail to conceive.

Nurhusien Yimer; Y. Rosnina; H. Wahid; A. A. Saharee; K. C. Yap; P. Ganesamurthi


Pakistan Veterinary Journal | 2012

Corpora lutea diameter, plasma progesterone concentration and follicular development in PGF2α and CIDR estrus synchronized goats.

M. M. Bukar; Y. Rosnina; O. M. Ariff; H. Wahid; G. K. M. A. Khan; Nurhusien Yimer; G. K. Dhaliwal

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Y. Rosnina

Universiti Putra Malaysia

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Nurhusien Yimer

Universiti Putra Malaysia

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K. C. Yap

Universiti Putra Malaysia

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M. Dashtizad

Universiti Putra Malaysia

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A. A. Saharee

Universiti Putra Malaysia

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M.R. Jainudeen

Universiti Putra Malaysia

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P. Ganesamurthi

Universiti Putra Malaysia

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H. Hajarian

Universiti Putra Malaysia

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A.B. Zuki

Universiti Putra Malaysia

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Kazhal Sarsaifi

Universiti Putra Malaysia

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