Habibur Rahman Bhuiyan

ORGANOCHLORINE INSECTICIDES (DDT AND HEPTACHLOR) IN DRY FISH AVAILABLE IN BANGLADESH: SEASONAL TRENDS AND SPECIES VARIABILITY

ABSTRACT The concentrations of organochlorine insecticides DDT (Dichloro diphenyl trichloro ethane) and Heptachlor were investigated to estimate the current status of insecticides used in dry fish in different seasons and different species. Six most popular species of dry fishes namely Bombay duck (Loittya), Ribbon fish (Chhuri), Shrimp (Chingri), Hilsha shed (Ilish), Chinese pomfret (Rupchanda) and Indian salmon (Lakhua) were collected from Asadgonj (whole sell market for dry fish) of Chittagong, Bangladesh at different seasons, six samples at winter season (December) and six same samples at rainy season (July). The range of DDT concentration at winter was found 3.6 ug kg-1 to 250.8 ug kg-1 and at rainy season the range of concentration was found 11.1 ug kg-1 to 1107.4 ug kg-1. The range of Heptachlor concentration at winter was found 0.4 ug kg-1 to 2.5 ug kg-1 and at rainy season was found 1.1 ug kg-1 to 37.8 ug kg-1. The concentrations of DDT and Heptachlor were much higher in the samples of rainy season than the same samples of the winter.

Simultaneous determination of amoxicillin and chloramphenicol and their drug interaction study by the validated UPLC method

Abstract A new UPLC method was developed for the simultaneous estimation of amoxicillin and chloramphenicol in pharmaceutical formulations. Drugs were resolved on Shim-Pack XR-ODS (2 mm i.d. × 50 mm, particle size 2.2 μm) reverse-phase column, utilizing a mobile phase of methanol:0.0025 M ammonium acetate in the initial ratio 40:60 (v/v) at a gradient program. The mobile phase was pumped at a flow rate of 0.25 mL/min with detection at 220 nm. 10 μL volume of sample was injected by the auto sampler. Separation was completed within 2.72 ± 0.03 min. Calibration curves were linear with correlation coefficient 0.999 over a studied concentration range of 10 μg mL−1 for both drugs. Method was found to be reproducible with relative standard deviation (RSD) for intra- and interday precision to be <1.5% over the said concentration range. The mean recovery of the drug from the standard solution was 99.39%. LOD was found to be 0.03 and 0.025 μg mL−1 for amoxicillin and chloramphenicol, respectively. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of amoxicillin and chloramphenicol in bulk and pharmaceutical formulations. Furthermore, study revealed that adverse drug interaction due to their concomitant use is not from pharmaceutical interaction.