Hacı Hasan Esen

Haemostatic role and histopathological effects of a new haemostatic agent in a rat bladder haemorrhage model: an experimental trial

To investigate the haemostatic efficacy and histopathological effects of a new haemostatic agent, Ankaferd BloodStopper® (ABS; Ankaferd Drug Cosmetic Co., Istanbul, Turkey) in a rat bladder haemorrhage model. ABS is a unique combination of five plant extracts that has been used in Turkish traditional medicine as a haemostatic agent for external traumatic bleeds.

Juvenile Ossifying Fibroma of the Mandible: a Case Report

ABSTRACT Background Fibro-osseous lesions of the jaws, including juvenile ossifying fibroma, pose diagnostic and therapeutic difficulties due to their clinical, radiological and histological variability. The aim of this study was to report the outcome of a 9 years old girl with diagnosed juvenile ossifying fibroma treatment. Methods A 9 years old girl presented with a 6 x 8 cm sized hard fixed tumour on right ramus and corpus of the mandible. On the radiological examination tumour showed an irregular but well bordered, unilocular and expansive lesion on the right corpus and ramus of the mandible. There was no teeth displacement or teeth root resorbtion. Microscopically, the tumour had trabeculae, fibrillary osteoid and woven bone. After the clinical, radiological (panoramic radiography, computed tomography and magnetic resonance imaging) and histologic analysis it was diagnosed juvenile ossifying fibroma. In the history of the patient there has been an acute lymphocytic leukaemia in the remission for 3 years. Results Because of large size of mandibular tumour, resultant expansion and destruction of mandibular cortex, the patient underwent right hemimandibulectomy using transmandibular approach. There was no recurrence or complications for two years follow-up. Conclusions Although juvenile ossifying fibroma is an uncommon clinical entity, its aggressive local behaviour and high recurrence rate means that it is important to make an early diagnosis, apply the appropriate treatment and, especially, follow-up the patient over the long-term.

An early diagnostic tool for diabetic neuropathy: conduction velocity distribution.

Diabetes is a metabolic disorder that affects much of the human population. As a secondary complication, diabetic neuropathy causes time‐dependent damage to peripheral nerves. In this study, experimental diabetes was induced by streptozotocin (STZ; 50 mg/kg intraperitoneally) in rats. Diabetic animals were grouped into those with 2 or 4 weeks of diabetes, whereas a control group received only the STZ vehicle (0.1 M citrate). Sciatic nerves were dissected, and compound action potentials (CAPs) were recorded. Results deduced by conventional calculation carried less information when compared with conduction velocity distribution (CVD) obtained by a computer‐based mathematical model. Using the conventional approach, statistically significant changes were first seen in the fourth week of diabetes, whereas results deduced by CVD measurement could be seen in the second week. Consequently, the CVD calculation provides more information for the early diagnosis of neuropathies compared with classical conduction velocity measurements. Muscle Nerve 43: 237–244, 2011

Spontaneous Infarction in a Fibroadenoma of the Breast.

Background: Fibroadenomas are common neoplasms in young women but occur in a wide age range from adolescent females to octogenians. Case Report: A 21-year-old female patient presented with a 10-week history of a mass in her breast. Ultrasound examination demonstrated a 3.5 × 3 cm, well-circumscribed, semisolid mass. An excisional biopsy but no fine needle aspiration was performed. The patient had no history of pregnancy or lactation, or trauma or infection to the area of the lesion. The histopathological examination showed a spontaneously infarcted fibroadenoma. Conclusions: Spontaneous infarction is an uncommon complication in fibroadenoma of the breast, and there are very few reported cases in the literature.

The influence of rifamycin decontamination on incorporation of autologous onlay bone grafts in rats: A histometric and immunohistochemical evaluation

OBJECTIVE Although it has been shown that rifamycin is an effective agent for bone graft decontamination, no information exists on the effects of rifamycin decontamination on bone graft incorporation. The aim of this study was to evaluate the influence of rifamycin decontamination on the incorporation of autologous onlay bone grafts quantitatively. DESIGN In 30 rats, a standardized 5.0-mm-diameter bone graft was harvested from the right mandibular angle, contaminated with saliva, decontaminated with rifamycin solution, and augmented to the left as an onlay graft. Ten animals were sacrificed at 7, 14, and 21 days after surgery. In the control group (10 rats), the onlay grafts were neither contaminated nor decontaminated, and the rats were sacrificed at 21 days after surgery. Histological slides were prepared from each grafted site for both immunohistochemistry analysis (bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) antibodies) and histometric analysis. Images obtained from the graft incorporation area with the light microscope were transferred to a PC, and they were evaluated using Clemex PE 3.5 image analysis software. RESULTS The grafts were fully incorporated in all specimens. The results showed that rifamycin decontamination has no detrimental effect on graft incorporation and the findings revealed a tendency for earlier revascularization and osteogenesis in the decontamination group. Data were analyzed using variance analysis and Tukeys test. CONCLUSIONS Rifamycin decontamination has no detrimental effect on autogenous graft incorporation, and it can be used for graft decontamination with confidence.

Gastrointestinal stromal tümörlerde prognozun belirlenmesinde preoperatif nötrofil lenfosit oranının etkisi

OBJECTIVE Gastrointestinal stromal tumors are believed to play a role in immunosuppression with their local effect. In some cancers with associated inflammation, an increased pretreatment neutrophil lymphocyte ratio is considered as an indicator of poor prognosis. The aim of our study was to assess the relationship of increased blood neutrophil lymphocyte ratio with disease prognosis in patients with gastrointestinal stromal tumors. MATERIAL AND METHODS The data of 78 patients who underwent surgery with the diagnosis of primary gastrointestinal stromal tumor in our clinic were evaluated retrospectively. The preoperative neutrophil lymphocyte ratio in the peripheral blood was determined. The neutrophil lymphocyte ratio and its relationship with tumor risk and prognosis were compared. The data were evaluated by Pearsons correlation analysis and the Welch ANOVA test. RESULTS The preoperative neutrophil lymphocyte ratio was significantly increased in the high-risk groups (p<0.05). An increased preoperative neutrophil lymphocyte ratio was associated with shorter survival (r=-0.32, p=0.009). In addition, an increase in the neutrophil lymphocyte ratio was associated with an increase in the mitotic activity of the tumor (r=-0.364, p=0.025). CONCLUSION The preoperative neutrophil lymphocyte ratio in gastrointestinal stromal tumors can be used as an indicator of high-risk tumors and poor prognosis.

EFFECT OF LOCAL RIFAMYCIN APPLICATION ON EXPRESSION OF BMP-2 AND BONE REGENERATION

Amac: Bu calismanin amaci lokal rifamisin uygulamasinin kemik iyilesmesi sirasinda BMP–2 salinimi uzerine etkisinin degerlendirilmesidir. Materyal ve method: Rat mandibula angulus bolgesinde standart olarak 5 mm capinda kritik boyutta kemik defektleri olusturulmustur. Kontrol grubunda (8 rat) defektlere herhangi bir uygulama yapilmamistir. Birinci deney grubunda (8 rat) defekt bolgesi rifamisin solusyonu ile irrige edildikten sonra, defekt bolgesine 1, 3 ve 7. gunlerde 25 mg rifamisin solusyonu enjekte edilmistir. Ikinci deney grubunda (8 rat) defekt bolgesi 25 mg rifamisin solusyonu ile karistirilmis gelatin sponge ile greftlenmistir. Cerrahiden 21 gun sonra ratlar sakrifiye edilmistir. Defekt bolgesinden hem immunhistokimyasal analiz (kemik morfogenetik protein –2 antibody) icin hem de histomorfometrik analiz icin histolojik kesitler hazirlanmistir. Elde edilen verilerin analizi Mann Whitney U ve Kruskall Wallis testi kullanilarak yapilmistir. Bulgular: Deney grubunda kontrol grubuna gore ortalama yeni kemik formasyonu, osteoblast sayisi ve yeni damar olusum sayisinda artis oldugu gorulmustur. Her iki deney grubunda da anti–bmp–2 ile isaretlenmenin (hucre sayma) kontrol grubuna gore daha fazla oldugu gorulmustur. Sonuc: Kritik boyutta kemik defektlerine lokal olarak rifamisin uygulamasinin BMP–2 salinimi uzerine pozitif etkileri oldugu tespit edilmistir.

Invasive papillary carcinoma of the male breast

Breast cancer is rarely seen in male patients. All the histological types of female breast cancer can be seen in male patients. Invasive papillary carcinoma is a rare breast cancer type. We hereby report the case of a 67-year-old male patient who underwent modified radical mastectomy because of invasive papillary carcinoma. The patient presented to our clinic with the complaint of a painless mass in his right breast. Tru-cut biopsy guided by imaging was performed. The pathological results of the biopsy were in line with invasive papillary carcinoma. We performed modified radical mastectomy on the patient. No recurrences were seen in the 24-month follow-up period.

Kemikte Metastatik Nazofarenks Karsinomunun Aspirasyon Sitolojisi ve Hücre Bloğu Bulguları- Olgu Sunumu

Nazofarenks karsinomu, nazofarenks epitelinden kaynaklanan ve tum malign hastaliklarin %0.2’ sini olusturan nadir bir tumordur. Uzak metastazlari akcigerleri, iskelet sistemi ve karacigeri icerebilir. Sitolojik inceleme, ozellikle hucre blogu bulgulari metastatik nazofarenks karsinomu teshisinde faydalidir. Andiferansiye nazofarenks karsinomu olan ve Iliak kemik metastazi bulunan olguyu sunduk ve metastatik odaktan hazirlanan aspirasyon sitolojisi ve hucre blogunun bulgularini vurguladik.