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Dive into the research topics where Hafiza Shukor is active.

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Featured researches published by Hafiza Shukor.


Bioresource Technology | 2014

Production of butanol by Clostridium saccharoperbutylacetonicum N1-4 from palm kernel cake in acetone–butanol–ethanol fermentation using an empirical model

Hafiza Shukor; Najeeb Kaid Nasser Al-Shorgani; Peyman Abdeshahian; Aidil Abdul Hamid; Nurina Anuar; Norliza Abd Rahman; Mohd Sahaid Kalil

Palm kernel cake (PKC) was used for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in acetone-butanol-ethanol (ABE) fermentation. PKC was subjected to acid hydrolysis pretreatment and hydrolysates released were detoxified by XAD-4 resin. The effect of pH, temperature and inoculum size on butanol production was evaluated using an empirical model. Twenty ABE fermentations were run according to an experimental design. Experimental results revealed that XAD-4 resin removed 50% furfural and 77.42% hydroxymethyl furfural. The analysis of the empirical model showed that linear effect of inoculums size with quadratic effect of pH and inoculum size influenced butanol production at 99% probability level (P<0.01). The optimum conditions for butanol production were pH 6.28, temperature of 28°C and inoculum size of 15.9%. ABE fermentation was carried out under optimum conditions which 0.1g/L butanol was obtained. Butanol production was enhanced by diluting PKC hydrolysate up to 70% in which 3.59g/L butanol was produced.


Bioresource Technology | 2016

Saccharification of polysaccharide content of palm kernel cake using enzymatic catalysis for production of biobutanol in acetone–butanol–ethanol fermentation

Hafiza Shukor; Peyman Abdeshahian; Najeeb Kaid Nasser Al-Shorgani; Aidil Abdul Hamid; Norliza Abd Rahman; Mohd Sahaid Kalil

In this work, hydrolysis of cellulose and hemicellulose content of palm kernel cake (PKC) by different types of hydrolytic enzymes was studied to evaluate monomeric sugars released for production of biobutanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in acetone-butanol-ethanol (ABE) fermentation. Experimental results revealed that when PKC was hydrolyzed by mixed β-glucosidase, cellulase and mannanase, a total simple sugars of 87.81±4.78 g/L were produced, which resulted in 3.75±0.18 g/L butanol and 6.44±0.43 g/L ABE at 168 h fermentation. In order to increase saccharolytic efficiency of enzymatic treatment, PKC was pretreated by liquid hot water before performing enzymatic hydrolysis. Test results showed that total reducing sugars were enhanced to 97.81±1.29 g/L with elevated production of butanol and ABE up to 4.15±1.18 and 7.12±2.06 g/L, respectively which represented an A:B:E ratio of 7:11:1.


Bioresource Technology | 2016

Enhanced mannan-derived fermentable sugars of palm kernel cake by mannanase-catalyzed hydrolysis for production of biobutanol

Hafiza Shukor; Peyman Abdeshahian; Najeeb Kaid Nasser Al-Shorgani; Aidil Abdul Hamid; Norliza Abd Rahman; Mohd Sahaid Kalil

Catalytic depolymerization of mannan composition of palm kernel cake (PKC) by mannanase was optimized to enhance the release of mannan-derived monomeric sugars for further application in acetone-butanol-ethanol (ABE) fermentation. Efficiency of enzymatic hydrolysis of PKC was studied by evaluating effects of PKC concentration, mannanase loading, hydrolysis pH value, reaction temperature and hydrolysis time on production of fermentable sugars using one-way analysis of variance (ANOVA). The ANOVA results revealed that all factors studied had highly significant effects on total sugar liberated (P<0.01). The optimum conditions for PKC hydrolysis were 20% (w/v) PKC concentration, 5% (w/w) mannanase loading, hydrolysis pH 4.5, 45°C temperature and 72h hydrolysis time. Enzymatic experiments in optimum conditions revealed total fermentable sugars of 71.54±2.54g/L were produced including 67.47±2.51g/L mannose and 2.94±0.03g/L glucose. ABE fermentation of sugar hydrolysate by Clostridium saccharoperbutylacetonicum N1-4 resulted in 3.27±1.003g/L biobutanol.


Saudi Journal of Biological Sciences | 2016

Enhanced butanol production by optimization of medium parameters using Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani; Hafiza Shukor; Peyman Abdeshahian; Mohd Sahaid Kalil; Wan Mohtar Wan Yusoff; Aidil Abdul Hamid

A new isolate of the solvent-producing Clostridium acetobutylicum YM1 was used to produce butanol in batch culture fermentation. The effects of glucose concentration, butyric acid addition and C/N ratio were studied conventionally (one-factor-at-a-time). Moreover, the interactions between glucose concentration, butyric acid addition and C/N ratio were further investigated to optimize butanol production using response surface methodology (RSM). A central composite design was applied, and a polynomial regression model with a quadratic term was used to analyze the experimental data using analysis of variance (ANOVA). ANOVA revealed that the model was highly significant (p < 0.0001) and the effects of the glucose and butyric acid concentrations on butanol production were significant. The model validation experiment showed 13.82 g/L butanol was produced under optimum conditions. Scale up fermentation in optimized medium resulted in 17 g/L of butanol and 21.71 g/L of ABE. The experimental data of scale up in 5 L bioreactor and flask scale were fitted to kinetic mathematical models published in the literature to estimate the kinetic parameters of the fermentation. The models used gave the best fit for butanol production, biomass and glucose consumption for both flask scale and bioreactor scale up.


Anaerobe | 2015

Improvement of the butanol production selectivity and butanol to acetone ratio (B:A) by addition of electron carriers in the batch culture of a new local isolate of Clostridium acetobutylicum YM1

Najeeb Kaid Nasser Al-Shorgani; Mohd Sahaid Kalil; Wan Mohtar Wan Yusoff; Hafiza Shukor; Aidil Abdul Hamid

Improvement in the butanol production selectivity or enhanced butanol:acetone ratio (B:A) is desirable in acetone-butanol-ethanol (ABE) fermentation by Clostridium strains. In this study, artificial electron carriers were added to the fermentation medium of a new isolate of Clostridium acetobutylicum YM1 in order to improve the butanol yield and B:A ratio. The results revealed that medium supplementation with electron carriers changed the metabolism flux of electron and carbon in ABE fermentation by YM1. A decrease in acetone production, which subsequently improved the B:A ratio, was observed. Further improvement in the butanol production and B:A ratios were obtained when the fermentation medium was supplemented with butyric acid. The maximum butanol production (18.20 ± 1.38 g/L) was gained when a combination of methyl red and butyric acid was added. Although the addition of benzyl viologen (0.1 mM) and butyric acid resulted in high a B:A ratio of 16:1 (800% increment compared with the conventional 2:1 ratio), the addition of benzyl viologen to the culture after 4 h resulted in the production of 18.05 g/L butanol. Manipulating the metabolic flux to butanol through the addition of electron carriers could become an alternative strategy to achieve higher butanol productivity and improve the B:A ratio.


International Journal of Hydrogen Energy | 2014

The production of biohydrogen by a novel strain Clostridium sp. YM1 in dark fermentation process

Peyman Abdeshahian; Najeeb Kaid Nasser Al-Shorgani; Noura K.M. Salih; Hafiza Shukor; Abudukeremu Kadier; Aidil Abdul Hamid; Mohd Sahaid Kalil


Biocatalysis and agricultural biotechnology | 2015

Process optimization of butanol production by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using palm oil mill effluent in acetone–butanol–ethanol fermentation

Najeeb Kaid Nasser Al-Shorgani; Hafiza Shukor; Peyman Abdeshahian; Mohd Yusuf Mohd Nazir; Mohd Sahaid Kalil; Aidil Abdul Hamid; Wan Mohtar Wan Yusoff


Renewable Energy | 2016

Utilization of palm kernel cake as a renewable feedstock for fermentative hydrogen production

Nadia Farhana Azman; Peyman Abdeshahian; Abudukeremu Kadier; Hafiza Shukor; Najeeb Kaid Nasser Al-Shorgani; Aidil Abdul Hamid; Mohd Sahaid Kalil


Bioresources | 2014

Biobutanol Production from Palm Kernel Cake (PKC) using Clostridium saccharoperbutylacetonicum N1-4 in Batch Culture Fermentation

Hafiza Shukor; Najeeb Kaid Nasser Al-Shorgani; Peyman Abdeshahian; Aidil Abdul Hamid; Nurina Anuar; Norliza Abd Rahman; Mohd Hafez Mohd Isa; Mohd Sahaid Kalil


International Journal on Advanced Science, Engineering and Information Technology | 2013

Pre-Optimization Conditions for Haematococcus pluvialis Growth

Nurul Asmidar Hanan; Najeeb Kaid Nasser Al-Shorgani; Hafiza Shukor; Norliza Abd Rahman; Mohd Sahaid Kalil

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Mohd Sahaid Kalil

National University of Malaysia

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Aidil Abdul Hamid

National University of Malaysia

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Norliza Abd Rahman

National University of Malaysia

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Nurina Anuar

National University of Malaysia

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Wan Mohtar Wan Yusoff

National University of Malaysia

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Abudukeremu Kadier

National University of Malaysia

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Asmidar Hanan

National University of Malaysia

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Mohd Hafez Mohd Isa

Universiti Sains Islam Malaysia

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