Hai Binh Nguyen

Graphene patterned polyaniline-based biosensor for glucose detection

This paper describes a glucose electrochemical biosensor, layer-by-layer fabricated from graphene and polyaniline films. Graphene sheets (0.5?0.5?cm2) with the thickness of 5?nm (15 layers) were synthesized by thermal chemical vapor deposition (CVD) under ambient pressure on copper tapes. Then they were transferred into integrated Fe3O4-doped polyaniline (PANi) based microelectrodes. The properties of the nanocomposite films were thoroughly characterized by scanning electron microscopy (SEM), Raman spectroscopy, atomic force microscopy (AFM) and electrochemical methods, such as square wave voltametry (SWV) and chronoamperometry. The above graphene patterned sensor (denoted as Graphene/Fe3O4/PANi/GOx) shows much improved glucose sensitivity (as high as 47??A?mM?1?cm?2) compared to a non-graphene one (10?30??A?mM?1?cm?2, as previously reported in the literature). It can be expected that this proof-of-concept biosensor could be extended for other highly sensitive biodetection.

Development of the layer-by-layer biosensor using graphene films: application for cholesterol determination

The preparation and characterization of graphene films for cholesterol determination are described. The graphene films were synthesized by thermal chemical vapor deposition (CVD) method. Methane gas (CH4) and copper tape were used as carbon source and catalyst in the graphene growth process, respectively. The intergrated array was fabricated by using micro-electro-mechanical systems (MEMS) technology in which Fe3O4-doped polyaniline (PANi) film was electropolymerized on Pt/Gr electrodes. The properties of the Pt/Gr/PANi/Fe3O4 films were investigated by field-emission scanning electron microscopy (FE-SEM), Raman spectroscopy and electrochemical techniques. Cholesterol oxidase (ChOx) has been immobilized onto the working electrode with glutaraldehyde agent. The cholesterol electrochemical biosensor shows high sensitivity (74 μA mM−1 cm−2) and fast response time (<5 s). A linear calibration plot was obtained in the wide cholesterol concentration range from 2 to 20 mM and correlation coefficient square (R2) of 0.9986. This new layer-by-layer biosensor based on graphene films promises many practical applications.

A novel nanofiber Cur-loaded polylactic acid constructed by electrospinning

Curcumin (Cur), extracted from the Curcuma longa L. plant, is well known for its anti-tumor, anti-oxidant, anti-inflammatory and anti-bacterial properties. Nanofiber mats of polylactic acid (PLA) loading Cur (5?wt%) were fabricated by electrospinning (e-spinning). Morphology and structure of the fibers were characterized by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FTIR) spectroscopy, respectively. The diameters of the obtained fibers varied from 200 to 300?nm. The release capacity of curcumin from curcumin-loaded PLA fibers was investigated in phosphate buffer saline (PBS) containing ethanol. After 24?h, 50% of the curcumin was released from curcumin-loaded PLA fibers. These results of electrospun (e-spun) fibers exhibit the potential for biomedical application.

Portable cholesterol detection with polyaniline-carbon nanotube film based interdigitated electrodes

Polyaniline-carboxylic multiwalled carbon nanotubes composite film (PANi-MWCNT) has been polymerized on the surface of interdigitated platinum electrode (fabricated by MEMS technology) which was compatibly connected to Autolab interface via universal serial bus (USB). An amperometric biosensor based on covalent immobilization of cholesterol oxidase (ChOx) on PANi?MWCNT film with potassium ferricyanide (FeCN) as the redox mediator was developed. The mediator helps to shuttle the electrons between the immobilized ChOx and the PANi-MWCNT electrode, therefore operating at a low potential of ?0.3?V compared to the saturated calomel electrode (SCE). This potential precludes the interfering compounds from oxidization. The bio-electrode exhibits good linearity from 0.02 to 1.2?mM cholesterol concentration with a correlation coefficient of 0.9985.

Some biomedical applications of chitosan-based hybrid nanomaterials

Being naturally abundant resources and having many interesting physicochemical and biological properties, chitin/chitosan have been found to be useful in many fields, especially biomedical ones. This paper describes the strategy to design multifunctional, hybrid chitosan-based nanomaterials and test them in some typical biomedical applications.

Electrosynthesis of polyaniline?mutilwalled carbon nanotube nanocomposite films in the presence of sodium dodecyl sulfate for glucose biosensing

Polyaniline–mutilwalled carbon nanotube (PANi–MWCNT) nanocomposites were electropolymerized in the presence of sodium dodecyl sulfate (SDS) onto interdigitated platinum-film planar microelectrodes (IDμE). The MWCNTs were first dispersed in SDS solution then mixed with aniline and H2SO4. This mixture was used to electro-synthesize PANi–MWCNT films with potentiostatic method at E = + 0.90 V (versus SCE). The PANi–MWCNT films were characterized by cyclic voltammetry (CV) and scanning electron microscopy (SEM). The results show that the PANi–MWCNT films have a high electroactivity, and a porous and branched structure that can increase the specific surface area for biosensing application. In this work the PANi–MWCNT films were applied for covalent immobilization of glucose oxidase (GOx) via glutaraldehyde agent. The GOx/PANi–MWCNT/IDμE was studied using cyclic voltammetric and chronoamperometric techniques. The effect of several interferences, such as ascorbic acid (AA), uric acid (UA), and acetaminophen (AAP) on the glucosensing at +0.6 V (versus SCE) is not significant. The time required to reach 95% of the maximum steady-state current was less than 5 s. A linear range of the calibration curve for the glucose concentration lies between 1 and 12 mM which is a suitable level in the human body.

Fabrication of few-layer graphene film based field effect transistor and its application for trace-detection of herbicide atrazine

We describe the fabrication of highly sensitive graphene-based field effect transistor (FET) enzymatic biosensor for trace-detection of atrazine. The few-layers graphene films were prepared on polycrystalline copper foils by atmospheric pressure chemical vapor deposition method using an argon/hydrogen/methane mixture. The characteristics of graphene films were investigated by scanning electron microscopy, transmission electron microscopy and Raman spectroscopy. The results indicated low uniformity of graphene layers, which is probably induced by heterogeneous distribution of graphene nucleation sites on the Cu surface. The pesticide detection is accomplished through the measurement of the drain-source current variations of the FET sensor upon the urea enzymatic hydrolysis reaction. The obtained biosensor is able to detect atrazine with a sensitivity of 56 μA/logCATZ in range between 2 × 10−4 and 20 ppb and has a limit of detection as low as 0.05 ppt. The elaboration of such highly sensitive biosensors will provide better biosensing performances for the detection of biochemical targets.

High-sensitivity planar Hall sensor based on simple gaint magneto resistance NiFe/Cu/NiFe structure for biochip application

The planar Hall effect (PHE) sensor based on a simple giant magneto resistance (GMR) trilayer structure NiFe/Cu/NiFe has been designed and fabricated successfully using conventional clean room fabrication methods. The PHE sensor is integrated by 24 sensor patterns with dimensions of 50 × 50 μm. Influence of individual layer thickness to sensitivity of sensor has been investigated. Sensitivity and planar Hall voltage increases with the decrease of Cu-layer thickness. The results are discussed in terms of the reinforcement of the antiferromagnetic interaction between NiFe layers and shunting current through the layer Cu. The optimum configuration has been found in the structure with the Cu-layer of 1 nm. In this case a single planar Hall effect sensor exhibits a high sensitivity of about 8 μV Oe−1 and a maximal of the signal change as large as ▵V ~ 55 μV. These values are comparable to those of the typical PHE sensor based on complex GMR spin-valve structure. With a high sensitivity and simple structure, this sensor is very promising for practical detection of magnetic beads and identifying multiple biological agents in the environment.

Influence of CoFe and NiFe pinned layers on sensitivity of planar Hall biosensors based on spin-valve structures

This paper deals with the magnetization, magnetoresistance and planar Hall effect (PHE) of NiFe(10)/Cu(1.2)/NiFe(tp)/IrMn(15) (nm) and NiFe(10)/Cu(1.2)/CoFe(tp)/IrMn(15) (nm) spin-valve structures with various thicknesses of pinned layer tp = 2, 6, 9, 12nm and a fixed free layer NiFe of tf = 10nm. Experimental investigations are performed for 50◊50µm junctions fabricated using lithography technique. The results show that the thinner the pinned layers, the higher is the PHE sensitivity obtained in both systems. In addition, in the spin-valve structures with the same pinned layer thickness, the CoFe-based system exhibits higher magnetoresistive ratio, but lower PHE sensitivity with respect to those of the FeNi-based system. The results are discussed in terms of the spin twist as well as the coherent rotation of the magnetization in the individual ferromagnetic layers. The highest PHE sensitivity S of 110µV(kAm 1 ) 1 has been obtained in the FeNi-based spin-valve structure with tp = 2nm.