Haibo Huang

Dielectrophoresis for bioparticle manipulation.

As an ideal method to manipulate biological particles, the dielectrophoresis (DEP) technique has been widely used in clinical diagnosis, disease treatment, drug development, immunoassays, cell sorting, etc. This review summarizes the research in the field of bioparticle manipulation based on DEP techniques. Firstly, the basic principle of DEP and its classical theories are introduced in brief; Secondly, a detailed introduction on the DEP technique used for bioparticle manipulation is presented, in which the applications are classified into five fields: capturing bioparticles to specific regions, focusing bioparticles in the sample, characterizing biomolecular interaction and detecting microorganism, pairing cells for electrofusion and separating different kinds of bioparticles; Thirdly, the effect of DEP on bioparticle viability is analyzed; Finally, the DEP techniques are summarized and future trends in bioparticle manipulation are suggested.

Optimal design of a new structure piezo-driven cell injector

With the development of micromanipulation technique, the piezo-driven cell injector has been widely applied in cell microinjection. Traditional injection technology used piezo actuator to drive pipettes and a small mercury column was applied in micropipette to diminish the lateral tip oscillations. This injector significantly improves the survival rates of the ICSI process, but large lateral tip oscillations of the micropipette and the toxicity of mercury may damage to the cell membrane with a lower survival rate. Based on the theoretical basis of the previous generation injection structure and conventional injection system, a new design of the piezo-driven microinjector is proposed for microinjection. In this paper, the new structure uses packaged piezo actuator as driving source for connecting more stable and reliable. And it adopts two points flexible way to fix the pipette instead of the way of one point. The design parameters have been optimized for meeting the requirements of small cell injection. Its more simple and easy to use. This paper used simulation software to study the lateral vibration of the new structure, and optimized design size for minimum the lateral oscillation of micropipettes. Harmful lateral tip oscillations of micropipette are reduced substantially during simulation. Finally, we make the new microinjector by 3D printing technology for future experiment. This new structure will reduce the damage to cells in a large extent with a high success rate.

Adherent cells localization based on Gaussian filtering in automated microinjection system

This work is based on the cells in the microinjection system to process adherent cells image. Performing low-pass filtering to the Image of adherent cells, removing uneven illumination, segmenting foreground and background, removing invalid small areas, operating opening and closing operation, keeping the target area, calibrating centers of the cells in the original image.

A novel multi-cue based image fusion method combining multi-principle microscopy images towards Nuclear transfer technology

Since its invention, the Nuclear transfer technology has a wide range of applications in fields such as animal breeding, gene therapy, organ transplants, etc.. However, since the oosight genetic material is not visible, presently the nucleus removal process of recipient oocyte always take the blind suction method, which led to extremely low success rate of nuclear transfer operation. Polarized light microscopy imaging system is very suitable for non -invasive imaging of living oocyte spindle. However, comparing to inverted microscopic imaging, it lost a lot of operating environment information (such as precision positioning of manipulation tools and cellular texture). In this paper, the advantages of polarized light microscopy imaging systems and traditional optical inverted microscope imaging system are combined. The fusion of images from polarized light microscopy imaging systems and traditional optical inverted microscope imaging system was realized. Collecting the polarized light microscopy image and inverted microscope image of same oosights in real time using two CCD cameras. A multi-cue image fusion method is proposed in this manuscript, experimental results show that this method is expected to achieve real-time visualized operation and observation.

Alginate gel electro-deposition for cell writing

In this paper, we presented electro deposition of calcium alginate for cell writing directly. The calcium alginate is generated on the surface of anode, which is immersed in mixed solution of sodium alginate and CaCO3 powder, when charged with electricity. The thickness of gel, which is produced on the anode surface, increases along with enlargement of current. At last, we mixed yeast cells in the sodium alginate solution (1%) with suspended CaCO3 particles (0.5%) to observe the growth situation of yeast cells.

The corresponding relationship research between human lower limb operation mode and muscle information

Surface EMG (sEMG) with correlation exists between the active and functional status of the muscle can be a very good reaction to neuromuscular activity. Studies concerning this has important significance to the development of research on rehabilitation robot. This paper aims to study the action pattern recognition technology. We can obtain the characteristic value of lower limb EMG signal and pattern recognition with the time and the level of excitement with the muscles corresponding to the lower extremity operation mode. The first step to deal with the collected muscles sEMG is to conduct a denoising pretreatment, and then use the Power spectral ratio method to obtain a characteristic value of each muscle. Finally, make a BP neural network to the obtained features so that we can identify corresponding relationship between the sEMG and the lower extremity operation mode. It was found that the excitement of the time and the level of excitement are different for each muscle sEMG. sEMG have similar activity in the same mode, and a clear distinction in the different operating modes. In a different operation mode, conduct a pattern recognition to the characteristic value of the surface EMG, and the operating mode thereof can be discriminated.

A novel shape information based spindle positioning method with the polarized light microscopy

Cell structures analyze and cell organelles play important roles in bio-manipulation. However, it is difficult to image the live cell organelles without damage by using the common microscopy. Polarized light microscopy provides unique opportunities for analyzing the architectural dynamics of living cells, tissues, and whole organisms. In this paper, a novel cell spindle positioning method with the polarized light is introduced. Firstly, the live cell spindle apparatus structure imaging method with the polarized light is presented; secondly, a novel shape information based spindle positioning method is introduced; then, cell spindle positioning experiments are carried out, experimental results shows the proposed method is valid..