Halina Szadkowski
University of Basel
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Featured researches published by Halina Szadkowski.
Journal of Molecular Biology | 1980
Kasper Kirschner; Halina Szadkowski; Agnes Henschen; Friedrich Lottspeich
Abstract The native enzyme must be denatured either by sodium dodecyl sulfate or by urea before limited proteolysis can occur. Under these conditions only one or two peptide bonds are hydrolyzed by each of the following proteases: Staphylococcal V8 protease, trypsin and elastase. The amino-terminal amino acid sequences were determined to identify the cleavage sites. The new sequences comprise approximately 20% of the entire polypeptide chain, and show good agreement with the nucleotide sequence of the trpC gene. Both V8 protease † and elastase yield large carboxy-terminal fragments, about two thirds of the size of the parent enzyme, and corresponding small amino-terminal fragments. Trypsin cleaves a single peptide bond in the last one third of the polypeptide chain. After separation of the fragments, removal of dodecyl sulfate and renaturation, only the large fragments fold to stable structures. The small fragments precipitate. The large amino-terminal fragment catalyzes only the synthesis of indoleglycerol phosphate and precipitates when solutions are frozen and thawed. The large carboxy-terminal fragment catalyzes only the isomerization of N-(5′-phosphoribosyl)anthranilate and is stable towards freezing and thawing. These studies prove that the intact bifunctional enzyme consists of two autonomously folding, functional domains. They also support the notion that the bifunctional enzyme may have arisen by the fusion of separate ancestral genes, and that stabilization of the intrinsically labile indoleglycerol phosphate synthase domain by interdomain interactions is functionally advantageous.
FEBS Journal | 1990
Konrad Ibel; Roland P. May; Kasper Kirschner; Halina Szadkowski; Erik Mascher; Per Lundahl
Biochemistry | 2000
Astrid Merz; Muh-Ching Yee; Halina Szadkowski; Günter Pappenberger; Andreas Crameri; Willem P. C. Stemmer; Charles Yanofsky; Kasper Kirschner
Protein Science | 1996
Reinhard Sterner; G. R. Kleemann; Halina Szadkowski; Ariel Lustig; Michael Hennig; Kasper Kirschner
Biochemistry | 1992
Anne Buchwalder; Halina Szadkowski; Kasper Kirschner
Methods in Enzymology | 1987
Kasper Kirschner; Halina Szadkowski; Theodore S. Jardetzky; Vreni Hager
Protein Engineering | 1990
Karolin Luger; Halina Szadkowski; Kasper Kirschner
FEBS Journal | 2002
Andreas Ivens; Olga Mayans; Halina Szadkowski; Catharina Jürgens; Matthias Wilmanns; Kasper Kirschner
Protein Science | 1998
Beatrice Darimont; Catherine Stehlin; Halina Szadkowski; Kasper Kirschner
FEBS Journal | 2001
Andreas Ivens; Olga Mayans; Halina Szadkowski; Matthias Wilmanns; Kasper Kirschner