Hamed Safafar
Technical University of Denmark
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Publication
Featured researches published by Hamed Safafar.
Marine Drugs | 2015
Hamed Safafar; Jonathan Van Wagenen; Per Møller; Charlotte Jacobsen
This study aimed at investigating the potential of microalgae species grown on industrial waste water as a new source of natural antioxidants. Six microalgae from different classes, including Phaeodactylum sp. (Bacillariophyceae), Nannochloropsis sp. (Eustigmatophyceae), Chlorella sp., Dunaniella sp., and Desmodesmus sp. (Chlorophyta), were screened for their antioxidant properties using different in vitro assays. Natural antioxidants, including pigments, phenolics, and tocopherols, were measured in methanolic extracts of microalgae biomass. Highest and lowest concentrations of pigments, phenolic compounds, and tocopherols were found in Desmodesmus sp. and Phaeodactylum tricornuotom microalgae species, respectively. The results of each assay were correlated to the content of natural antioxidants in microalgae biomass. Phenolic compounds were found as major contributors to the antioxidant activity in all antioxidant tests while carotenoids were found to contribute to the 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity, ferrous reduction power (FRAP), and ABTS-radical scavenging capacity activity. Desmodesmus sp. biomass represented a potentially rich source of natural antioxidants, such as carotenoids (lutein), tocopherols, and phenolic compounds when cultivated on industrial waste water as the main nutrient source.
Food Chemistry | 2016
Nina Gringer; Hamed Safafar; Axelle du Mesnildot; Henrik Hauch Nielsen; Adelina Rogowska-Wrzesinska; Ingrid Undeland; Caroline P. Baron
This study aimed at unravelling the antioxidative capacity of low molecular weight compounds (LMWC) (peptides, amino acids and phenolic acids) present in salt brines from the marinated herring production. Brines were fractionated into <10kDa fractions using dialysis and further into 94 fractions using size exclusion chromatography. All samples were analysed for protein, total phenolic content (TPC) and antioxidant activities. Protein-enriched samples were pooled (P1, P2 and P3) and analysed for phenolic acids, total amino acids and peptide/protein sequence using advanced mass spectrometry. All salt brines contain LMWC holding ABTS-radical scavenging activity, reducing power and iron chelating activity. Generally, a strong correlation between TPC and ABTS-radical scavenging was found. In contrast, reducing power and iron chelating activity seemed to be caused by peptides. Protein/peptide sequencing revealed 1kDa peptides with the presence of HDF-motif which could be responsible for some of the antioxidant capacity observed in marinated herring salt brine.
Marine Drugs | 2016
Hamed Safafar; Michael Z. Hass; Per Møller; Susan Løvstad Holdt; Charlotte Jacobsen
Nannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis salina biomass, with a focus on eicosapentaenoic acid (EPA). Variations in fatty acid composition, lipids, protein, amino acids, tocopherols and pigments were studied and results compared to algae cultivated on F/2 media as reference. Mixed growth media and process water enhanced the nutritional quality of Nannochloropsis salina in laboratory scale when compared to algae cultivated in standard F/2 medium. Data from laboratory scale translated to the large scale using a 4000 L flat panel photo-bioreactor system. The algae growth rate in winter conditions in Denmark was slow, but results revealed that large-scale cultivation of Nannochloropsis salina at these conditions could improve the nutritional properties such as EPA, tocopherol, protein and carotenoids compared to laboratory-scale cultivated microalgae. EPA reached 44.2% ± 2.30% of total fatty acids, and α-tocopherol reached 431 ± 28 µg/g of biomass dry weight after 21 days of cultivation. Variations in chemical compositions of Nannochloropsis salina were studied during the course of cultivation. Nannochloropsis salina can be presented as a good candidate for winter time cultivation in Denmark. The resulting biomass is a rich source of EPA and also a good source of protein (amino acids), tocopherols and carotenoids for potential use in aquaculture feed industry.
Journal of Marine Science and Engineering | 2016
Hamed Safafar; Patrick Uldall Nørregaard; Anita Ljubic; Per Møller; Susan Løvstad Holdt; Charlotte Jacobsen
Archive | 2017
Hamed Safafar; Charlotte Jacobsen; Per Møller
Journal of Applied Phycology | 2018
Anita Ljubic; Hamed Safafar; Susan Løvstad Holdt; Charlotte Jacobsen
Journal of Applied Phycology | 2018
Goncalo Silva Marinho; Ann-Dorit Moltke Sørensen; Hamed Safafar; Anja H. Pedersen; Susan Løvstad Holdt
European Journal of Lipid Science and Technology | 2017
Hamed Safafar; Sten Langvad; Peter Møller; Charlotte Jacobsen
22nd International Seaweed Symposium | 2016
Ann-Dorit Moltke Sørensen; Hamed Safafar; Anja Pedersen; Goncalo Silva Marinho; Susan Løvstad Holdt
Harnessing Marine Bioresources for Innovations in the Food Industry | 2015
Hamed Safafar; Charlotte Jacobsen; Per Møller