Hamid SadAbadi

Integration of gold nanoparticles in PDMS microfluidics for lab-on-a-chip plasmonic biosensing of growth hormones

Gold nanoparticles were synthesized in a poly(dimethylsiloxane) (PDMS) microfluidic chip by using an in-situ method, on the basis of reductive properties of the cross-linking agent of PDMS. The proposed integrated device was further used as a sensitive and low-cost LSPR-based biosensor for the detection of polypeptides. Synthesis of nanoparticles in the microfluidic environment resulted in improvement of size distribution with only 8% variation, compared with the macro-environment that yields about 67% variation in size. The chemical kinetics of the in-situ reaction in the microfluidic environment was studied in detail and compared with the reaction carried out at the macro-scale. The effect of temperature and gold precursor concentration on the kinetics of the reaction was investigated and the apparent activation energy was estimated to be Ea*=30 kJ/mol. The sensitivity test revealed that the proposed sensor has a high sensitivity of 74 nm/RIU to the surrounding medium. The sensing of bovine growth hormone also known as bovine somatotropin (bST) shows that the proposed biosensor can reach a detection limit of as low as 3.7 ng/ml (185 pM). The results demonstrate the successful integration of microfluidics and nanoparticles which provides a potential alternative for protein detection in clinical diagnostics.

Nano-Integrated Suspended Polymeric Microfluidics (SPMF) Platform for Ultra-Sensitive Bio-Molecular Recognition of Bovine Growth Hormones

The development of sensitive platforms for the detection of biomolecules recognition is an extremely important problem in clinical diagnostics. In microcantilever (MC) transducers, surface-stress is induced upon bimolecular interaction which is translated into MC deflection. This paper presents a cost-effective and ultra-sensitive MC-based biosensing platform. To address these goals, the need for costly high-resolution read-out system has been eliminated by reducing the cantilever compliance through developing a polymer-based cantilever. Furthermore a microfluidic system has been integrated with the MC in order to enhance sensitivity and response time and to reduce analytes consumption. Gold nanoparticles (AuNPs) are synthesized on the surface of suspended microfluidics as the selective layer for biomolecule immobilization. The biosensing results show significant improvement in the sensitivity of the proposed platform compared with available silicon MC biosensor. A detection limit of 2 ng/ml (100pM) is obtained for the detection of bovine growth hormones. The results validated successful application of suspended polymeric microfluidics (SPMF) as the next generation of biosensing platforms which could enable femtomolar (fM) biomolecular recognition detection.

Effects of Electrode Switching Sequence on EWOD Droplet Manipulation: A Simulation Study

Electrowetting-on-dielectric (EWOD) is a new method for handling droplets on the microfluidic chips. By applying electrical potential, the interfacial energy of liquid-solid interface changes, results altering of droplet contact lines. To increase the flow rate of such a digital microfluidic system one way is to raise the droplet velocity. One important factor for enhancing droplet velocity in EWOD systems is the proper switching the electrodes or “switching sequence”. To examine the effect of switching in EWOD, the EDEW 1.0 simulation tool is used in this paper. By simulating the motion of a 1μL water droplet in a 1D electrode array, the resultant surface energy curves during the motion of droplet in different electrode switching sequences are obtained. The results show proper electrode switching has a remarkable effect on increasing of droplet velocity. To enhance the droplet velocity, the electrode, which is placed next to the droplet at forward direction, should be powered after droplet passed over it. In addition, it would be more efficient to first turn on the next electrode, and then turn off the previous one.Copyright

Sensitive, Real-time and Non-Intrusive Detection of Concentration and Growth of Pathogenic Bacteria using Microfluidic-Microwave Ring Resonator Biosensor

Infection diagnosis and antibiotic susceptibility testing (AST) are time-consuming and often laborious clinical practices. This paper presents a microwave-microfluidic biosensor for rapid, contactless and non-invasive device for testing the concentration and growth of Escherichia Coli (E. Coli) in medium solutions of different pH to increase the efficacy of clinical microbiology practices. The thin layer interface between the microfluidic channel and the microwave resonator significantly enhanced the detection sensitivity. The microfluidic chip, fabricated using standard soft lithography, was injected with bacterial samples and incorporated with a microwave microstrip ring resonator sensor with an operation frequency of 2.5 GHz and initial quality factor of 83 for detecting the concentration and growth of bacteria. The resonator had a coupling gap area on of 1.5 × 1.5 mm2 as of its sensitive region. The presence of different concentrations of bacteria in different pH solutions were detected via screening the changes in resonant amplitude and frequency responses of the microwave system. The sensor device demonstrated near immediate response to changes in the concentration of bacteria and maximum sensitivity of 3.4 MHz compared to a logarithm value of bacteria concentration. The minimum prepared optical transparency of bacteria was tested at an OD600 value of 0.003. The sensor’s resonant frequency and amplitude parameters were utilized to monitor bacteria growth during a 500-minute time frame, which demonstrated a stable response with respect to detecting the bacterial proliferation. A highly linear response was demonstrated for detecting bacteria concentration at various pH values. The growth of bacteria analyzed over the resonator showed an exponential growth curve with respect to time and concurred with the lag-log-stationary-death model of cell growth. This biosensor is one step forward to automate the complex AST workflow of clinical microbiology laboratories for rapid and automated detection of bacteria as well as screening the bacteria proliferation in response to antibiotics.

Uniform integration of gold nanoparticles in PDMS microfluidics with 3D micromixing

The integration of gold nanoparticles (AuNPs) on the surface of polydimethylsiloxane (PDMS) microfluidics for biosensing applications is a challenging task. In this paper we address this issue by integration of pre-synthesized AuNPs (in a microreactor) into a microfluidic system. This method explored the affinity of AuNPs toward the PDMS surface so that the pre-synthesized particles will be adsorbed onto the channel walls. AuNPs were synthesized inside a microreactor before integration. In order to improve the size uniformity of the synthesized AuNPs and also to provide full mixing of reactants, a 3D-micromixer was designed, fabricated and then integrated with the microreactor in a single platform. SEM and UV/Vis spectroscopy were used to characterize the AuNPs on the PDMS surface.

Simulation and Dynamic Characterization of a 3-Layer Piezo-Actuated Valveless Micropump System

In order to design the valveless micropump with a Piezo actuator, it is essential to understand the dynamic properties of the actuating system. Besides several other considerations in designing of microfluidic systems, the efficiency of valveless micropumps also strongly depends on parameters of the actuation system including the actuation frequency. Cleary, higher displacement of the diaphragm results in higher output flow rate of the pump. Thus, studying the dynamic behavior of the actuation system forms one of the important considerations for the design of micropumps. Three different models of the actuating system for the fabricated micropump system are proposed with different boundary conditions and are simulated by finite element method using ANSYS. Comparison of the experimental results and the simulation results of the natural frequencies of the system shows that the proposed simulation method can also be now used as a tool to optimize the design of the actuation system in terms of natural frequency of the system.Copyright