Hanna Bis-Wencel

Effort of Varying Intensity as a Factor Influencing the Variability of Selected Biochemical Blood Parameters of Jumping Horses

Abstract Blood samples were collected from horses in four time points of the spring-winter training season, according to the difference in effort intensity characteristic for each period. Serum contents of glucose, cholesterol, and creatinine, as well as activity of creatine kinase, aspartate and alanine aminotransferases, and alkaline phosphatase were determined. Training did not cause muscle damage, and as a result of training, changes in the characteristics of both, contraction and biochemical properties of muscle tissue took place, which was confirmed by the conducted tests.

Ghrelin concentration in half-blood jumping horses during moderate and intense training exercise

Abstract The aim of the study was to assess the impact of varying intensity of exercise on ghrelin levels in half-blood jumping horses during training season. Blood samples were collected in the period of intense and moderate exercise. Serum concentrations of active and total ghrelin were measured using commercial RIA kits. Exercise did not cause the disruption in energy homeostasis, which was confirmed by the conducted tests. Total concentration of ghrelin measured during the increased effort intensity elevated in stallions (from 1031.45 to 1732.19 pg/mL at P ≤ 0.05). The opposite situation occurred in the case of active ghrelin, which concentration did not increase significantly in mares (from 26.21 to 28.46 pg/mL), whereas an insignificant decrease was noted in stallions (from 24.19 to 22.54 pg/mL). The only significant difference between stallions and mares was noted in the 2nd sampling (P = 0.015). The research proved that the fluctuations of both studied parameters did not reflect a negative impact of the increased effort.

Tissue pathomorphology and immunohistochemistry in mink (Neovison vison) fed blood plasma supplemented diet in the period of preparation for breeding

Abstract The research carried out at a mink farm aimed to determine the effect of blood plasma supplemented diet applied at the period preparing mink for reproduction on the animal organism. The studies included four groups of mink. The control group received a non-supplemented diet, while the experimental groups had feed with additive of 0.5%, 1.5%, and 2.5% of beef-pork plasma in the daily feed ration. The pathomorphological and immunohistochemical evaluation was performed on the liver, kidneys, lymph nodes, spleen, and bowel from all the groups. Pathomorphological and immunohistochemical changes of various intensity were observed in the examined organs from all experimental groups.