Hans-Joachim Guder

Fluorescence Polarisation Immunoassay

Since the introduction of radio immunoassay techniques [1], many different assay principles based on antibodies as specifiers have become routine tools in clinical diagnostics. Heterogeneous immunoassays, e.g. enzyme linked immunosorbant assays (ELISA) [2], require a series of time consuming incubation and wash steps, but they are characterized by a high sensitivity with a detection limit of 10−13 moll−1 and even lower.

Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems

A large number of detection systems for biomolecules have been described in the literature; therefore, only a brief overview of the most important nonradioactive methods will be given here. More details can be found in the other chapters of this book or in the cited literature.