Hans Pillekamp

Eosinophilic fasciitis treated with psoralen-ultraviolet A bath photochemotherapy

Eosinophilic fasciitis is a rare disorder which can markedly affect the quality of life in individual patients. So far, no generally accepted and effective treatment modality has been available. Although the precise nature of eosinophilic fasciitis is still unknown, it is often regarded as a variant of localized scleroderma (morphoea). Phototherapy and photochemotherapy have been shown to be effective in the treatment of sclerodermatous skin lesions. We report a patient with eosinophilic fasciitis which was successfully treated with psoralen plus ultraviolet A bath photochemotherapy within 6 months.

Late-type allergy to the X-ray contrast medium Solutrast (iopamidol).

In the past few years, there have been an increasing number of publications on delayed intolerance reactions, including rashes, following the use of X‐ray contrast media. We report a patient in whom infiltrated erythema of the face and generalized maculopapular rashes occurred on 2 occasions, within 1 day, following the use of the X‐ray contrast medium Solutrast® (iopamidol) for coronary angiography. The allergological investigations for clarification included prick tests and patch tests using a series of contrast media, as well as individual intravenous provocation tests. We found the cause to be a late‐type allergy to the active substance iopamidol contained in the contrast medium Solutrast®. We found a concomitant cross‐reactivity to the contrast media iopromid and iomeprol. All 3 contrast media represent the monomeric, non‐ionic type.

Calcipotriol vs. tazarotene as combination therapy with narrowband ultraviolet B (311 nm): efficacy in patients with severe psoriasis.

Background Phototherapy has been shown to be one of the most effective treatment modalities for patients with psoriasis. Nevertheless, photocombination therapies capable both of reducing cumulative ultraviolet (UV) doses and of accelerating clearance of skin lesions are important and of high interest. There have been no published studies comparing the effect of narrowband UVB irradiation in combination with topical application of tazarotene vs. calcipotriol.

PUVA-gel vs. PUVA-bath therapy for severe recalcitrant palmoplantar dermatoses. A randomized, single-blinded prospective study.

Background/Purpose: In order to avoid unwanted effects of systemic psoralen and ultraviolet A (PUVA) therapy, various topical PUVA treatment modalities have been developed and are being increasingly used. However, up to now very few controlled studies comparing the therapeutic efficacy of different topical photochemotherapy modalities are available. Thus, the aim of our study was to compare the clinical efficacy of conventional PUVA‐bath therapy to topical PUVA‐gel therapy in patients with recalcitrant dermatoses of the palms and soles.

Radiation-Induced Late Effects in Two Affected Individuals of the Lilo Radiation Accident

Abstract Scherthan, H., Abend, M., Müller, K., Beinke, C., Braselmann, H., Zitzelsberger, H., Köhn, F. M., Pillekamp, H., Schiener, R., Das, O., Peter, R. U., Herzog, G., Tzschach, A., Dörr, H. D., Fliedner, T. M. and Meineke, V. Radiation-Induced Late Effects in Two Affected Individuals of the Lilo Radiation Accident. Radiat. Res. 167, 615–623 (2007). Radiation exposure leads to a risk for long-term deterministic and stochastic late effects. Two individuals exposed to protracted photon radiation in the radiological accident at the Lilo Military site in Georgia in 1997 received follow-up treatment and resection of several chronic radiation ulcers in the Bundeswehr Hospital Ulm, Germany, in 2003. Multi-parameter analysis revealed that spermatogenetic arrest and serum hormone levels in both patients had recovered compared to the status in 1997. However, we observed a persistence of altered T-cell ratios, increased ICAM1 and β1-integrin expression, and aberrant bone marrow cells and lymphocytes with significantly increased translocations 6 years after the accident. This investigation thus identified altered end points still detectable years after the accident that suggest persistent genomic damage as well as epigenetic effects in these individuals, which may be associated with an elevated risk for the development of further late effects. Our observations further suggest the development of a chronic radiation syndrome and indicate follow-up parameters in radiation victims.

Occupational protein contact dermatitis caused by meat and fish

Background Protein contact dermatitis is a form of contact dermatitis possibly triggered by proteinaceous allergens.

Does the minimal phototoxic dose after 8‐methoxypsoralen baths correlate with the individual’s skin phototype?

Background/Aims: Up to now no data have been available concerning whether there is a significant correlation between skin phototypes and the minimum phototoxic dose (MPD) after bath water delivery of 8‐MOP.

Can the immersion time of PUVA bath therapy be shortened

Up to now, there are only a few data available concerning the influence of bathing time on skin phototoxicity. We compared the erythemal responses of normal skin to bath PUVA with 8-methoxypsoralen (8-MOP) after 5, 10 and 20 min immersion time. Currently, 20 min is the routinely performed immersion time in many European countries, including Germany, while in other countries bathing times are shorter. The minimal phototoxic dose (MPD) following immersion times of 5 min and 10 min in a warm water bath (37 degrees C) containing 1 mg/l 8-MOP was compared to the MPD following 20 min immersion time in a half-sided manner in a total of 24 patients. Our results revealed that an immersion time of 5 min did not yield a detectable erythema after 72 h. In contrast, both 10 and 20 min PUVA baths induced visible erythemas with a significantly higher median MPD following 10 min immersion (2.25 J/cm2) compared to 20 min baths (1.5 J/cm2). As an erythemal response of 8-MOP PUVA bath seems reduced after shorter immersion times, comparative studies on the clinical efficacy using shorter time regimens have to be conducted before conclusive recommendations for clinical PUVA-bathing time can be given.

Skin-explant model to evaluate effectiveness of depletion of hla-alloreactive t-cells

Abstract Depletion of host specific alloreactive T-cells from donor lymphocytes is an attractive tool for donor lymphocyte infusions after stem cell grafting. We intended to establish a preclinical method to evaluate effectiveness and safety of this approach. The skin explant model is a powerful method to predict the risk of GvHD in the HLA-identical setting, and we adapted this model to the HLA-haploidentical situation. Unmanipulated donor T-cells are compared with donor T-cells following depletion of host specific T-cells. Prior to depletion donor MNC are incubated in the presence of irradiated recipient MNC and after a period of 5 days activated T-cells, positive for CD25, CD69, CD71 and HLA-DR, are magnetically removed (VarioMacs, Miltenyi). A repeat MLC of unmanipulated donor cells. Fresh skin biopsies are divided into pieces of 1 - 2 mm diameter and incubated with 10 6 cultured T-cells in 20% autologous serum for 72 hours, fixed in formaldehyde and stained with haematoxylin-eosin. Skin alterations are graded according to Learner et al. In 3 separate experiments undepleted cells caused subepidermal cleft formation corresponding to a grade III to IV GvHD reaction. In contrast, depleted cells showed only grade I alterations, as similarly observed in medium control. Our preliminary data suggest that this approach could represent a useful tool to determine effectiveness of magnetic depletion of alloreactive T-cells and be valuable to assess the safety of these cells prior to infusion.