Harold S. Fleming

Changes in the Teeth and Jaws of Neonatal Webster Mice After Administration of Naf aNd CaF2 to the Female Parent During Gestation

S EVERAL studies have shown that certain concentrations of fluoride appear capable of passing through placental membranes.3 7,10 In view of this fact, results of fluoride administration during pregnancy, as reflected in the teeth or adjacent structures of the newborn child or animal, should be of interest. It is the purpose of this paper to report findings as seen in certain teeth of neonatal Webster mice following administration of NaF and CaF2, in selected dilutions, to the female parents. Reports in the literature on the specific effects of fluoride administered during the gestation period of the female parent are lacking. Studies such as those of Hicks5 on the administration of metabolic inhibitors during gestation have usually been concerned with structures other than the teeth. The recent work of Kreshover and Clough5 approaches the problem of prenatal influences on tooth development by inducing maternal pyrexia.

The osteogenic tendency of dentine after formation in tooth germ transplants.

Abstract 1. 1. All dentine found in transplants of tooth germs ultimately changed into a structure that simulated bone. 2. 2. The rate and extent of this change varied but the end result always appeared to be the same. 3. 3. No osteoid tissue was induced at the site of implantation of tooth germs in intracerebral transfers as it was in the anterior eye chamber. 4. 4. Odontoblasts did not seem to take part in this change; but other cells among the odontoblasts and in the pulpal area demonstrated an osteogenic capacity.

Salivary gland tumors

Abstract One hundred twenty-three cases of salivary gland tumors treated at Grace-New Haven Community Hospital from 1922 to 1952 have been reviewed, and the findings are as follows. 1. 1. The parotid gland was the most frequent tumor site. 2. 2. Malignant and recurrent tumors were found most often in the parotid gland. 3. 3. All biopsied tumors recurred, with three exceptions. 4. 4. The tumor-free survival period of surgically treated recurrent tumors was low. 5. 5. Treatment was generally by surgery. X-ray therapy sometimes was used after surgery, but it was seldom used alone. 6. 6. A small group of patients with histologic benign tumors presented a problem from the point of therapy because of repeated recurrence. 7. 7. Standardization of histologic criteria would simplify and contribute much in comparative studies of these tumors.

Transplantation of Human Tooth Germs to Lower Animals I. Relation to the Host

PART of a previous report on the intraocular growth of transplanted tooth germs dealt in a small way with such transfers made from human beings (3 fetuses) to experimental animals.1 Since that time, scope of this work was broadened considerably by the availability of additional human material (28 embryos and fetuses), suitable for in vivo transplantation. Therefore, added significance can be attached to results obtained in these instances further supporting the original findings. It is the purpose of this communication to present these findings stressing at this time relation of the transplant to the host.

Shope papilloma virus and tooth germ transplants

Abstract Growth and development of transplants of species-susceptible tooth germs were altered, whether homotransplanted or heterotransplanted. With species-resistant tooth germs, whether transplants were made to a species-susceptible or a species-resistant strain, no alterations in expected growth and development were observed. The greatest effect was on the epithelial layers of the tooth germ transplants. Odontoblasts were also altered in their appearance and association with dentine. Both enamel and dentine were changed in appearance. Keratin formations and their manner of proliferation were strikingly similar to those induced by treating tooth germs with polycyclic carcinogenic hydrocarbons before transplantation.

Effect of methylcholanthrene on tooth germ transplants: additional studies.

A PREVIOUS investigation showed alteration in the development of tooth germs synchronously transplanted with methylcholanthrene.7 It was felt that further changes in the transplanted tooth germs might take place if the animals were held for longer periods, before sacrificing, than those used in the previous experiments. It was also felt that certain stains might better demonstrate the effect of methyleholanthrene on tooth germ transplants. It is the purpose of this paper to present these findings.

Effect of carcinogenic hydrocarbons on tooth germ homotransplants in female guinea pig castrates

Abstract 1. 1. There was less growth of carcinogenic-treated tooth germs than of untreated tooth germs in female guinea pig castrates. 2. 2. There was more resorption with carcinogenic-treated tooth germs in castrates than in noncastrates, and there was less resorption when diethylstilbestrol was injected. 3. 3. Inflammatory responses to treated tissues were greater in castrates than in intact hosts. 4. 4. Cellular responses by the hosts were changed when diethylstilbestrol was injected. 5. 5. Host vascular systems were active about transplants and increased when diethylstilbestrol was used. 6. 6. Angiomatous proliferations were marked in and about the transfers. 7. 7. Keratin formations were significantly decreased or were absent. 8. 8. Epithelial tissues proliferated more in castrates than similar tissues had been seen to do in intact hosts.