Haruhide Udagawa
Shimadzu Corp.
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Featured researches published by Haruhide Udagawa.
International Journal of Pharmaceutics | 2010
Takanori Kanazawa; Yuuki Takashima; Toshiaki Tamura; Miki Tsuchiya; Yasunori Shibata; Haruhide Udagawa; Hiroaki Okada
The vaginal mucosa is the most common site of initiation of virus infections that are transmitted through heterosexual intercourse, including HIV and papillomavirus. Thus, in order to prevent or treat these infections, strong vaginal immunity is required as the first line of defense. In this study, to establish a less invasive, safe, convenient and effective immunization method, we examined the local (skin and vagina) gene transfection efficiency of a non-needle jet injector for daily insulin injection. In the skin experiment, the needle-free injector resulted in a marked increase in marker gene expression, compared to the conventional needle-syringe injection. In addition, intradermal DNA vaccination using the needle-free injector dramatically induced IFN-gamma and antibody systemic responses in mice. Furthermore, we investigated the applicability of the needle-free injector as a vaginal vaccination tool in rabbits. Vaginal gene expression using the needle-free injector was significantly greater than that using needle-syringe injection. Moreover, intravaginal vaccination by the needle-free injector promoted vaginal IgA secretion and IFN-gamma mRNA expression in the blood lymphocytes, to a degree significantly higher than that by needle-syringe injection. In conclusion, local vaginal DNA vaccination using a needle-free jet injector is a promising approach for the prevention and treatment of mucosal infectious diseases.
Vaccine | 2010
Jun-ichi Imoto; Tomohiro Ishikawa; Atsushi Yamanaka; Misako Konishi; Kenji Murakami; Tomoyuki Shibahara; Masanori Kubo; Chang-Kweng Lim; Masataka Hamano; Tomohiko Takasaki; Ichiro Kurane; Haruhide Udagawa; Yoshihiro Mukuta; Eiji Konishi
Japanese encephalitis (JE) virus causes abortion and stillbirth in swine, and encephalitis in humans and horses. We have previously reported that immunogenicity of a DNA vaccine against JE was synergistically enhanced in mice by co-immunization with a commercial inactivated JE vaccine (JEVAX) under a needle-free injection system. Here, we found that this immunization strategy was also effective in miniature pigs. Because of the synergism, miniature pigs immunized twice with a mixture of 10 μg of DNA and a 1/100 dose of JEVAX developed a high neutralizing antibody titer (1:190 at 90% plaque reduction assay). Even using 1 μg of DNA, 3 of 4 pigs developed neutralizing antibodies. Following challenge, all miniature pigs with detectable neutralizing antibodies were protected against viremia. Pregnant sows inoculated with 10 or 1 μg of DNA mixed with JEVAX (1/100 dose) developed antibody titers of 1:40-1:320. Following challenge, fetal death and mummification were protected against in DNA/JEVAX-immunized sows.
international conference of the ieee engineering in medicine and biology society | 2011
Akihiro Ishikawa; Haruhide Udagawa; Yoshinori Masuda; Satoru Kohno; Takashi Amita; Yoshihiro Inoue
Brain-machine interfaces (BMI) are expected as new man-machine interfaces. Non-invasive BMI have the potential to improve the quality of life of many disabled individuals with safer operation. The non-invasive BMI using the functional functional near-infrared spectroscopy (fNIRS) with the electroencephalogram (EEG) has potential applicability beyond the restoration of lost movement and rehabilitation in paraplegics and would enable normal individuals to have direct brain control of external devices in their daily lives. To shift stage of the non-invasive BMI from laboratory to clinical, the key factor is to develop high-accuracy signal decoding technology and highly restrictive of the measurement area. In this article, we present the development of a high-accuracy brain activity measurement system by combining fNIRS and EEG. The new fNIRS had high performances with high spatial resolution using double density technique and a large number of measurement channels to cover a whole human brain.
Archive | 2009
Yoshihiro Inoue; Takashi Amita; Satoru Kohno; Akihiro Ishikawa; Yoshinori Masuda; Haruhide Udagawa
Archive | 2004
Ryuichi Morishita; Naruya Tomita; Yasuo Kunugiza; Yoshiaki Taniyama; Hiromi Koike; Haruhide Udagawa; Tatsuo Kimura
Archive | 2011
Haruhide Udagawa; Yoshihiro Inoue
Archive | 2012
Akihiro Ishikawa; Yoshihiro Inoue; Takashi Amita; Satoru Kohno; Haruhide Udagawa; Yoshinori Masuda
Archive | 2010
Yoshihiro Inoue; Takashi Amita; Satoru Kohno; Akihiro Ishikawa; Yoshinori Masuda; Haruhide Udagawa
Archive | 2010
Yoshihiro Inoue; Takashi Amita; Satoru Kohno; Akihiro Ishikawa; Yoshinori Masuda; Haruhide Udagawa
Archive | 2004
Tatsuo Kimura; Hiromi Koike; Yasuo Kunugiza; Ryuichi Morishita; Yoshiaki Taniyama; Naruya Tomita; Haruhide Udagawa; 奈留也 冨田; 晴英 宇田川; 弘美 小池; 辰男 木村; 竜一 森下; 康夫 椚座; 義明 谷山