Haruhisa Noda

Value of reverse transcription polymerase chain reaction assay in pathological stage T3N0 prostate cancer.

We tested the ability of the nested reverse transcription polymerase chain reaction (RT‐PCR) assay to detect signs of biochemical recurrence of prostate cancer in the lymph nodes and peripheral blood of patients with pT3N0 prostate cancer.

Comparisons of the Various Combinations of Free, Complexed, and Total Prostate–Specific Antigen for the Detection of Prostate Cancer

Objectives: We compared the ability of three prostate–specific antigen (PSA) ratios – free–to– total PSA ratio (fPSA/tPSA), free–to–complexed PSA ratio (fPSA/cPSA), and complexed–to–total PSA ratio (cPSA/tPSA) – to distinguish prostate cancer from benign prostatic hyperplasia (BPH).Methods: We tested 258 consecutive patients who underwent transrectal ultrasound–guided prostate needle biopsy because of an abnormal digital rectal examination or a Tandem–R PSA of >4.1 ng/ml. Free PSA (fPSA) and total PSA (tPSA) were measured by Tandem–R assay. α1–Antichymotrypsin–complexed PSA (cPSA) was measured by Markit–M PSA–ACT assay.Results: Of the 258 patients, 204 had BPH, and 54 had prostate cancer. The specificity at 96% sensitivity for fPSA/tPSA, fPSA/cPSA, and cPSA/tPSA was 23, 25, and 33%, respectively. Of 162 patients with tPSA between 4.1 and 10.0 ng/ml, 132 had BPH and 30 had prostate cancer. The specificity at 96% sensitivity for f/tPSA, f/cPSA and c/tPSA was 32, 44, and 41%, respectively. There was no significant difference in the area under the receiver–operating characteristic curves among fPSA/tPSA, fPSA/cPSA, and cPSA/tPSA in the overall PSA range or in tPSA between 4.1 and 10.0 ng/ml.Conclusion: fPSA/tPSA, fPSA/cPSA, and cPSA/tPSA did not differ in their ability to distinguish prostate cancer from BPH.

The significance of the free-to-complexed prostate-specific antigen (PSA) ratio in prostate cancer detection in patients with a PSA level of 4.1–10.0 ng/mL

Objective To compare the ratio of free prostate specific antigen (fPSA), total PSA (tPSA) and complexed PSA (cPSA, measured using a novel immunoassay) with other variables used to detect prostate cancer in patients with intermediate serum PSA levels of 4.1–10.0 ng/mL.

Comparison of transperitoneal and retroperitoneal laparoscopic nephrectomy for renal cell carcinoma: A single‐center experience of 100 cases

Objectives:  To report our experience with the retroperitoneal and transperitoneal approaches of laparoscopic nephrectomy for renal cell carcinoma (RCC).

Use of various combinations of free, complexed and total prostate-specific antigen levels as predictors of the pathologic stage of prostate cancer.

Abstract Background: The efficacy of various combinations of total, free and complexed prostate‐specific antigen (PSA) levels were assessed to predict the pathologic stage of prostate cancer.

Notice of Duplicate Publication

Upon close scrutiny of Figure 2 in the article by Webley and coworkers published in AJRCCM (1) and Figure 2 from the article by Cirino and coworkers published in BMC Infectious Diseases (2), we realized that the figure in BMC Infectious Diseases was a lower magnification of part of the same field as that used in the AJRCCM article. These figures both describe the growth of Chlamydia from clinical samples on the same tissue culture substrate (J774A.1 cells) and were in a single database of figures with different numeric designations. The experiments that led to the data for these two articles were generated around the same time and were compiled and stored in the same folder since pictures are taken in bulk at our microscopy facility where we pay a fee for the microscopy services. We believe that sometime during the compilation and annotation process, Figure 2 in the BMC Infectious Diseases article was incorrectly labeled as being generated from the normal donor project and was later used in that manuscript. In fact, this figure was taken from a culture coverslip that was infected with lysates from pediatric bronchial lavage samples. The AJRCCM figure was a 600 immunofluorescence image of a field on a coverslip, part of which can be seen in the lower magnification image (400 ) in the BMC Infectious Diseases article. The authors will supply the editor ofBMC Infectious Diseases with the appropriate representative culture image from normal donor infection of J774A.1 cells to replace the existing one. We deeply regret this error in image annotation and later image selection and hope that this will in no way detract from the important data contained in these two manuscripts.