Haruka Yamaguchi

Presence and Functionality of Mating Type Genes in the Supposedly Asexual Filamentous Fungus Aspergillus oryzae

ABSTRACT The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the reference strain RIB40. We now report the existence of a complementary MAT1-2 gene and the sequencing of an idiomorphic region from A. oryzae strain AO6. This allowed the development of a PCR diagnostic assay, which detected isolates of the MAT1-1 and MAT1-2 genotypes among 180 strains assayed, including industrial tane-koji isolates. Strains used for sake and miso production showed a near-1:1 ratio of the MAT1-1 and MAT1-2 mating types, whereas strains used for soy sauce production showed a significant bias toward the MAT1-2 mating type. MAT1-1 and MAT1-2 isogenic strains were then created by genetic manipulation of the resident idiomorph, and gene expression was compared by DNA microarray and quantitative real-time PCR (qRT-PCR) methodologies under conditions in which MAT genes were expressed. Thirty-three genes were found to be upregulated more than 10-fold in either the MAT1-1 host strain or the MAT1-2 gene replacement strain relative to each other, showing that both the MAT1-1 and MAT1-2 genes functionally regulate gene expression in A. oryzae in a mating type-dependent manner, the first such report for a supposedly asexual fungus. MAT1-1 expression specifically upregulated an α-pheromone precursor gene, but the functions of most of the genes affected were unknown. The results are consistent with a heterothallic breeding system in A. oryzae, and prospects for the discovery of a sexual cycle are discussed.

Experimental investigation of frictional melting of argillite at high slip rates: Implications for seismic slip in subduction-accretion complexes

[1] Discovery of pseudotachylytes from exhumed accretionary complexes indicates that frictional melting occurred along illite-rich, argillite-derived slip zones during subduction earthquakes. We conducted high-velocity friction experiments on argillite at a slip rate of 1.13 m/s and normal stresses of 2.67–13.33 MPa. Experiments show slip weakening followed by slip strengthening. Slip weakening is associated with the formation and shearing of low-viscosity melt patches. The subsequent slip strengthening occurred despite the reduction in shear strain rate due to the growth (thickening) of melt layer, suggesting that the viscosity of melt layer increased with slip. Microstructural and chemical analyses suggest that the viscosity increase during the slip strengthening is not due to an increase in the volume fraction of solid grains and bubbles in the melt layer but could be caused primarily by dehydration of the melt layer. Our experimental results suggest that viscous braking can be efficient at shallow depths of subduction-accretion complexes if substantial melt dehydration occurs on a timescale of seismic slip. Melt lubrication can possibly occur at greater depths within subduction-accretion complexes because the ratio of viscous shear to normal stress decreases with depth. Argillite-derived natural pseudotachylytes formed at seismogenic depths in subduction-accretion complexes are more hydrous than the experimentally generated pseudotachylytes and may be evidence of nearly complete stress drop.

Comparative rheological behaviour of albite and quartz in siliceous schists revealed by the microboudinage of piedmontite

Abstract This paper presents a detailed comparison of the microboudinage of piedmontite in two different mineralogical hosts, a quartz matrix, and albite porphyroblasts in a siliceous schist, with the aim of clarifying the rheological properties of albite in relation to those of quartz. Stress and strain analyses of the microboudinage confirm that the boudinage took place in the retrograde stage of metamorphism during decreasing temperature, and reveal that albite deformed at the same strain rate as quartz above the plastic-brittle transition temperature of albite.