Haruo Takeda

The establishment of a new mouse model with orthotopic esophageal cancer showing the esophageal stricture

We established a promising new experimental animal model with an orthotopic xenograft of esophageal cancer that successfully represents poor oral intake, a major clinical feature of esophageal cancer. The advantage of this model is that no surgical technique is required, only the injection of a cell suspension by a needle and syringe via the esophageal lumen from the mouth, which provides a high reproducibility of tumor implantation and a rapid progress of outcome. We propose that this model is useful to study cancer-related outcomes and for developing new therapies for esophageal cancer, and we expect it to make a contribution to clinical practice.

Dynamic 3D stabilization for video CG composite

We propose a method to estimate camera parameters from video sequences for the purpose of natural CG composite remarking the problem of stabilization. When the video is taken under wrong condition, it is inescapable that the estimated parameters contain nonnegligible errors which cause an unexpected shaking of CG object. To avoid this problem, we introduce a smoothing technique taking account of a property of synthesizing object. The method is based on an idea that the estimated camera parameters need not to be correct so far as composite is natural, and can be varied according to the condition of CG object even though the given images are same. Introducing the factor of locational and temporal smoothness to estimate the camera parameters, we can accomplish an appropriate stabilization for particular CG composite.

Image generation for immersive multi-screen environment with a motion ride

We have developed an immersive projection system which enables the viewer to experience a truly immersive feeling by looking at a wide-angle image on multiple surrounding screens. Our system is similar to previous systems but has a unique point in that it includes a new motion-based ride system as part of the environment. This ride system includes a seat that can be moved and rotated to give a rider (viewer) a sense of acceleration. We have used this environment to develop an application in which the viewer can experience a sensation of moving around in a virtual world while riding a virtually moving machine, such as a virtual vehicle.

Prolonged Epigastric Pain Caused by a Leaf Stem Embedded in the Gastric Wall

A 67-year-old woman underwent esophagogastroduodenoscopy to determine the cause of her prolonged epigastric pain. During the examination, a sharp-pointed foreign body was observed; the edge of the object had been embedded in the gastric wall. The object was removed via an endoscope, and the patients symptoms improved immediately. Based on a dietary history and an electron microscope examination, we identified the object as a stem of mizuna, a potherb mustard. Our report indicates that commonly eaten leafy green vegetables can act as sharp-pointed foreign bodies with the potential to injure the upper gastrointestinal tract.

Abstract 4165: The establishment of a new mouse model with orthotopic esophageal cancer showing the esophagostenosis

[Introduction] Esophageal cancer is one of the most incurable gastrointestinal diseases. In order to identify more effective therapeutic agents or strategies, the usage of established animal models is important to collect meaningful preclinical data. So far, a few rodent models bearing orthotopic esophageal cancer have been developed. However, high-skilled surgical techniques are necessary in these models and yet reproducibility needs to be improved, resulting in their insufficient popularity in esophageal cancer study. It is important that any novel model can represent clinical features of esophageal cancer progression, such as a loss of body weight and diet intake. In this study, we propose a new experimental animal model which represents clinical features and requires no surgical technique. [Matherials and Methods] TE4, TE8 cell lines and six week-old male BALB/cA-nu/nu mice were used in this study. The 5×106 cells suspension was mixed with Matrigel and injected into an anesthetized mouse. The procedure was as follows; 1) the anesthetized mouse was fixed on the table in a dorsal position, 2) a barrel, which was combined with a 22G needle, was inserted into the esophagus from the mouth, 3) the needle with a syringe containing the mixture of cell suspension and matrigel was then inserted into the barrel slowly, 4) when the needle tip was exposed from the barrel, the tip was thrust through the esophagus wall from the inside, 5) then the cell suspension was injected near the esophagus. These mice were examined for changes of body weight, dietary intake, and survival. For a study with chemotherapy, the tumor-bearing mice were randomized into 2 groups and either 5-FU (20 mg/kg 5 days/week 2 weeks) or PBS as a vehicle (control) was intraperitoneally administered from 7 days after cell injection. [Results] The implanted tumor located near the esophagus and the bronchus had invaded the adventitia of the esophagus in the microscopic observation. Computed tomography and 3-D images imaging revealed that the tumor compressed the esophagus and the bronchus, suggesting that a possible obstruction of the esophagus might occur in the mice. The mice bearing the implanted tumor experienced decreased body weight and food and water intake compared to the control group. The decrease of oral intake appeared to affect their lives, with a poor outcome of the orthotopic tumor-bearing mice in 40 days. 5-FU treatment allowed the mice to continue oral intake of food and water, accompanied with a maintaining of body weight. Treatment with this anti-cancer drug apparently prolonged their survival periods (5-FU treatment: 60.75 days; vehicle only:30.00 days, p [Conclusion] We established a promising new experimental animal model with an orthotopic xenograft of esophageal cancer that can well represent poor oral intake, a major clinical feature of esophageal cancer. This model is useful to make a contribution to clinical practice. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4165.